Biological molecule detecting method based on optical comb coherent imaging analysis

Abstract

The invention relates to a biological molecule detecting method based on optical comb coherent imaging analysis. According to the characteristics that molecules with different structures certainly have the same absorption spectrum, the wave coherent position of an absorption band and the strength of the absorption band reversely reflect features of the molecules on the structure, the biological molecule detecting method can be used for identifying a chemical group or the composition and structure of an unknown sample; and the absorption strength of the band corresponds to the content of the molecules, and therefore, the spectrum of an optical comb can carry out quantitative analysis and purity identification. Particularly by the measurement advantages of high reproducibility and high precision, the biological molecule detecting method can be used for spectrum calibration of millions of known biological molecules, and a spectral standard atlas database or a molecule fingerprint atlas database is established and is used as a 'fingerprint spectrum' of the biological molecules. The sample can be subjected to non-contact nondestructive detection. A pathogen is subjected to spectral analysis by a high-precision optical comb, the type and concentration of the pathogen can be recognized rapidly and accurately; and because the optical comb has a wide spectral range, the pathogen can bedetected at a time.

Description

technical field [0001] The invention belongs to the technical field of biomolecular detection, and in particular relates to a biomolecular detection method based on optical comb coherent imaging analysis. Background technique [0002] Many of the gold standards for clinical disease detection require invasive monitoring to obtain a diagnosis. For example, diabetes, acute and chronic renal failure all require blood system testing, and renal fibrosis detection even requires invasive puncture local surgery to achieve clarity. Moreover, the puncture site and the way of obtaining the specimen all affect the conclusion of the diagnosis. [0003] Diabetes is a syndrome caused by chronic hyperglycemia. It is a relatively common disease caused by insufficient insulin secretion. When insulin is insufficiently secreted, it first causes glucose metabolism disorders. Because of insufficient insulin, sugar enters cells and glycogen synthesis decreases. Reduced sugar alcoholysis, weakened ...

AI Technical Summary

Problems solved by technology

[0006] Since the concept of dual-comb spectroscopy was proposed, a large number of verification experiments have been done in the field of near-infrared and mid-infrared band research. Infrared absorption spectrum is an important "molecular fingerprint" to characterize the characteristics of gas molecules. Compared with the traditional Fourier transform spectrometer of Michelson interferometer, the dual-comb spectrometer has superior performance in terms of spectral resolution, sampling time and signal-to-noise ratio, but it also has the disadvantage of inconsistency in wavelength and cannot detect blood Qualitative or quantitative analysis of components and gas components

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