A kind of method adopting bioreactor to produce cultured root of wild ginseng

A bioreactor and root culture technology, applied in the field of plant tissue culture, can solve the problems of limiting ginseng production and supply, heavy metal residues, pollution, etc., and achieve the effects of avoiding pesticide residues, regulating blood pressure and blood lipids, and improving production efficiency

Active Publication Date: 2020-06-16
LIAONING PROSPECTIVE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Artificially planted ginseng is the most important source to meet market demand, but ginseng planting has special requirements on geographical location and environment. At the same time, ginseng with good health effects needs to be planted for more than six years and the cultivated land cannot be continuously cropped, which seriously limits the supply of ginseng production Another unavoidable shortcoming of artificial planting is the heavy metal residue caused by the use of pesticides and environmental pollution in the planting process to soil corrosion

Method used

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  • A kind of method adopting bioreactor to produce cultured root of wild ginseng
  • A kind of method adopting bioreactor to produce cultured root of wild ginseng
  • A kind of method adopting bioreactor to produce cultured root of wild ginseng

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Effect test

Embodiment 1

[0035] The identification of single nucleotide polymorphism (SNP, single nucleotide polymorphism) on the source of the cultured root of wild ginseng is mainly based on the electrophoresis results of nucleic acid fragments to distinguish it from common planted ginseng, and it is also confirmed that the cultured root of wild ginseng is indeed from the This century wild wild ginseng. Gene sequencing was carried out on three groups of allelic nucleic acid fragments of female wild ginseng, cultured roots and planted ginseng, and it was found that at the same gene allelic site, the nucleic acid sequences of female wild ginseng and the isolated cultured roots of female wild ginseng were guanine, Planted ginseng contains adenine. According to the sequence difference at this point, we designed a PCR amplification strategy. Three nucleic acid fragments can be amplified from the female wild ginseng and cultured roots, while only two nucleic acid fragments can be amplified from planted gin...

Embodiment 2

[0039] The specific production process is:

[0040] 1. Select a bioreactor: an air-lift bioreactor with a tank volume of 18 liters. The air-lift bioreactor is cylindrical in shape and has an internal volume of 18 liters; Placed on the bracket, each bracket can hold 18 reactors, which is called a set of air-lift reactors.

[0041] 2. 0.4g of fresh wild ginseng cultured root was first filled with 120mL of sucrose 4.2g and final concentration of 3ppm of IBA (indole butyric acid) in the Erlenmeyer flask of optimized SH liquid medium for 35 days. , so that the cultured root of wild ginseng is full of the whole triangular flask.

[0042] 3. Obtain a sufficient amount of cultured roots of wild ginseng through simultaneous cultivation in multiple conical flasks, collect 160 grams of wet weight cultured roots and inoculate them into 13 liters of SH standard liquid medium with a concentration ratio of 0.75 times and pre-moist heat sterilization. In the reactor; the temperature range o...

Embodiment 3

[0049] 1. Select the bioreactor: an airlift bioreactor with a tank volume of 18 liters.

[0050] 2. 0.5g of fresh wild ginseng cultured roots were first shaken and amplified for 32 days in the Erlenmeyer flask of the optimized SH liquid medium that was equipped with 120mL. The SH liquid medium contained 4.8 grams of sucrose and the IBA of final concentration 5ppm (ind indolebutyric acid).

[0051] 3. Obtain a sufficient amount of cultured roots of wild ginseng through simultaneous cultivation in multiple conical flasks, collect 180 grams of wet weight cultured roots and inoculate aseptically into a bioreactor equipped with 13 liters of SH medium with a concentration ratio of 0.75 times and pre-moist heat sterilization within; the culture temperature range is strictly controlled at 23-26°C, and the air ventilation range is strictly controlled at 0.10-0.20m 3 / h, the sucrose concentration in the medium is 3.5%, and the IBA concentration is 3.0ppm.

[0052] 4. Cultivate in the ...

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Abstract

The invention relates to a method for producing cultured ginseng roots by adopting a bioreactor. The method comprises the following steps: cutting fresh ginseng into pieces, carrying out induction culturing so as to obtain an explant callus, inducing the callus to grow adventitious root tissues called the cultured ginseng roots, and subjecting the cultured ginseng roots to multiplication culturingunder shaking in a triangular flask containing a sucrose and IBA contained optimized SH liquid culture medium until the whole triangular flask is filled with the cultured ginseng roots; and after thecompletion of culturing in the triangular flask, inoculating the cultured ginseng roots in an airlift type bioreactor, allowing the cultured ginseng roots to rapidly grow and propagate in the liquidculture medium in required temperature and ventilation control ranges, adding a growth promoter and a ginsenoside generating inducer in the growing process of the cultured ginseng roots, after the cultured ginseng roots are cultured in the bioreactor, and carrying out cleaning, soaking and drying so as to obtain a large number of finished products, i.e., the cultured ginseng roots. According to the invention, the growth promoter and the ginsenoside generating inducer are added through optimization, so the yield of the cultured ginseng roots and the total content of the ginsenoside are significantly improved.

Description

technical field [0001] The invention relates to a raw material that can be used for food and cosmetics, in particular to a method for rapidly and massively producing cultured roots of wild ginseng by using a bioreactor, and belongs to the technical field of plant tissue culture. Background technique [0002] Ginseng is a precious traditional Chinese medicine with the longest application history, belonging to the Panax genus. The meaning of Panax in Greek means "total medical treatment" or "panacea", which shows that ginseng has many functions that are widely recognized as beneficial to human health. Although ginseng can be divided into more than a dozen species in plant classification, only Korean ginseng (also known as Changbai ginseng or Jilin ginseng in China, Panax.ginseng C.A.Mayer) and American ginseng (also known as American ginseng) are the most accepted and edible or American ginseng, P.quinguefolius L.). Ginseng is popular all over the world as a traditional toni...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 侯剑英王晓东庄欠民
Owner LIAONING PROSPECTIVE BIOTECH
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