Method for extracting antibacterial components from dianthus chinensis and application of antibacterial components

An antibacterial ingredient, carnation technology, applied in the application field of antibacterial ingredients, to achieve good antibacterial activity

Inactive Publication Date: 2018-02-02
程志鹏
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few related studies on the extraction of Dianthus at present, and most of them only focus on the research on the saponins contained in it.

Method used

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  • Method for extracting antibacterial components from dianthus chinensis and application of antibacterial components
  • Method for extracting antibacterial components from dianthus chinensis and application of antibacterial components

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A method for extracting antibacterial components from Dianthus, comprising the following steps:

[0021] (1) Remove impurities from the whole Dianthus radix, dry at a constant temperature at 40-50°C until anhydrous, then pulverize and sieve to 50-60 mesh to obtain Dianthus powder;

[0022] (2) put carnation powder into the extraction kettle, add volume concentration and be 75% ethanol, control the liquid-solid ratio of ethanol and carnation powder to be 5:1; carry out supercritical CO 2 Extraction, extraction twice, the first extraction conditions are: extraction temperature 50°C, extraction pressure 18MPa, carbon dioxide flow rate 20L / h, extraction time 40min; second extraction conditions are: extraction temperature 45°C, extraction The pressure is 16MPa, the carbon dioxide flow rate is 15L / h, and the extraction time is 10min; the extracted extract is separated from the CO 2 , control the temperature of the separation kettle to be 32°C, the pressure to be 12MPa, and t...

Embodiment 2

[0026] A method for extracting antibacterial components from Dianthus, comprising the following steps:

[0027] (1) Remove impurities from the whole Dianthus radix, dry at a constant temperature at 40-50°C until anhydrous, then pulverize and sieve to 50-60 mesh to obtain Dianthus powder;

[0028] (2) put carnation powder into the extraction kettle, add volume concentration and be 80% ethanol, control the liquid-solid ratio of ethanol and carnation powder to be 6:1; carry out supercritical CO 2 Extraction, extraction twice, the first extraction conditions are: extraction temperature 55°C, extraction pressure 20MPa, carbon dioxide flow rate 25L / h, extraction time 45min; second extraction conditions are: extraction temperature 46°C, extraction The pressure is 18MPa, the carbon dioxide flow rate is 18L / h, and the extraction time is 12min; the extracted extract is separated from the CO 2 , control the temperature of the separation kettle to be 35°C, the pressure to be 16MPa, and t...

Embodiment 3

[0032] A method for extracting antibacterial components from Dianthus, comprising the following steps:

[0033] (1) Remove impurities from the whole Dianthus radix, dry at a constant temperature at 40-50°C until anhydrous, then pulverize and sieve to 50-60 mesh to obtain Dianthus powder;

[0034] (2) put carnation powder into the extraction kettle, add volume concentration and be 85% ethanol, control the liquid-solid ratio of ethanol and carnation powder to be 8:1; carry out supercritical CO 2 Extraction, extraction twice, the first extraction conditions are: extraction temperature 60°C, extraction pressure 25MPa, carbon dioxide flow rate 30L / h, extraction time 50min; second extraction conditions are: extraction temperature 48°C, extraction The pressure is 20MPa, the carbon dioxide flow rate is 20L / h, and the extraction time is 15min; the extracted extract is separated from the CO 2 , control the temperature of the separation kettle to be 36°C, the pressure to be 18MPa, and t...

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Abstract

The invention provides a method for extracting antibacterial components from Dianthus chinensis and the application of the antibacterial components, belonging to the technical field of Chinese herbal medicine extraction. The method includes the following steps: low-temperature drying and grinding of carnation to obtain carnation powder; supercritical CO2 extraction of carnation powder to obtain primary extract; ethyl acetate extraction and filtration of the primary extract, and silica gel column chromatography for the filtrate Separation, gradient elution with petroleum ether-ethyl acetate mixed solution; separation by silica gel column chromatography, isocratic elution with petroleum ether-ethyl acetate with a volume ratio of 20:80, and purification with a silica gel column, with volume The acetonitrile-acetone ratio is 40:60 for elution and the elution fraction is collected, concentrated and dried to obtain the antibacterial composition of caryophyllales. The present invention successfully extracts the antibacterial active ingredients from the carnation, and the acquisition of these active ingredients has positive significance for the research on the antibacterial property of the carnation.

Description

【Technical field】 [0001] The invention relates to the technical field of extracting Chinese herbal medicines, in particular to a method for extracting antibacterial components from dianthus and the application of the antibacterial components. 【Background technique】 [0002] With the widespread use of antibiotics, the drug resistance of bacteria is increasing year by year, becoming a "super bacteria" that is difficult for clinicians to deal with. At the same time, the toxic and side effects of antibiotics themselves pose a threat to the human body and the environment. Natural medicines are medicines developed from natural resources such as plants, animals and microorganisms, which have the advantages of less toxic side effects and less drug resistance. Currently, about 30% of the worldwide pharmaceutical market is derived from natural products. Searching for novel antibacterial drugs from natural products, especially from medicinal plants, has attracted the attention of res...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/36A61P31/04
CPCA61K36/36A61K2236/15A61K2236/333A61K2236/37A61K2236/39A61K2236/51A61K2236/55
Inventor 程志鹏
Owner 程志鹏
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