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Method for extracting and testing selenium polysaccharide from selenium-enriched lotus leaf lyophyllum mycelia

A technology of lotus leaves and selenium polysaccharides, applied in the field of biomedicine, can solve the problems of selenium polysaccharide molecular biology research and insufficient structure-activity relationship

Active Publication Date: 2018-02-06
HEXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the full functions and biochemical properties of selenium polysaccharides are not yet fully understood, and direct evidence and experiments on the molecular biology research, structure-activity relationship and mechanism of action of selenium polysaccharides are even more insufficient.

Method used

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  • Method for extracting and testing selenium polysaccharide from selenium-enriched lotus leaf lyophyllum mycelia
  • Method for extracting and testing selenium polysaccharide from selenium-enriched lotus leaf lyophyllum mycelia
  • Method for extracting and testing selenium polysaccharide from selenium-enriched lotus leaf lyophyllum mycelia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0090] A method for extracting selenium polysaccharides from the mycelium of the selenium-enriched lotus leaf Agrocybe, specifically including the following steps;

[0091] Step (1) Put the selenium-enriched lotus leaf licheniformis mycelium fermented in a 20L biological tank into a vacuum drying oven for drying, and grind through a 100-mesh sieve to prepare a sample;

[0092] Step (2) Accurately weigh 0.5000 g of the sample, and perform ultrasonic extraction once at an extraction time of 15 minutes, an extraction temperature of 50°C, and a material-to-liquid ratio of 1:40;

[0093] Step (3) After extraction, centrifuge at 3000r / min for 15min, combine the supernatant and concentrate under reduced pressure into a concentrated solution ≤50mL;

[0094] Step (4) Add 3 times the volume of 95% ethanol to the concentrated solution and let it stand overnight in a refrigerator at 4°C to obtain selenium polysaccharide precipitate. After repeated washing with ethanol, the selenium polysaccharide ...

Embodiment 2

[0097] A method for extracting selenium polysaccharides from the mycelium of the selenium-enriched lotus leaf Agrocybe, specifically including the following steps;

[0098] Step (1) Put the selenium-enriched lotus leaf licheniformis mycelium fermented in a 20L biological tank into a vacuum drying oven for drying, and grind through a 100-mesh sieve to prepare a sample;

[0099] Step (2) Accurately weigh 0.5000 g of the sample, and perform ultrasonic extraction twice under the conditions of extraction time of 50 minutes, extraction temperature of 83°C, and material-to-liquid ratio of 1:130;

[0100] Step (3) After extraction, centrifuge at 3000r / min for 15min, combine the supernatant and concentrate under reduced pressure into a concentrated solution ≤50mL;

[0101] Step (4) Add 3 times the volume of 95% ethanol to the concentrated solution and let it stand overnight in a refrigerator at 4°C to obtain selenium polysaccharide precipitate. After repeated washing with ethanol, the selenium ...

Embodiment 3

[0104] A method for extracting selenium polysaccharides from the mycelium of the selenium-enriched lotus leaf Agrocybe, specifically including the following steps;

[0105] Step (1) Put the selenium-enriched lotus leaf licheniformis mycelium fermented in a 20L biological tank into a vacuum drying oven for drying, and grind through a 100-mesh sieve to prepare a sample;

[0106] Step (2) Accurately weigh 0.5000g of the sample, and perform ultrasonic extraction 4 times under the conditions of extraction time 75min, extraction temperature 90°C, and material-to-liquid ratio 1:200;

[0107] Step (3) After extraction, centrifuge at 3000r / min for 15min, combine the supernatant and concentrate under reduced pressure into a concentrated solution ≤50mL;

[0108] Step (4) Add 3 times the volume of 95% ethanol to the concentrated solution and let it stand overnight in a refrigerator at 4°C to obtain selenium polysaccharide precipitate. After repeated washing with ethanol, the selenium polysaccharid...

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Abstract

The invention provides a method for extracting and testing selenium polysaccharide from selenium-enriched lotus leaf lyophyllum mycelia. The method concretely comprises the following steps of using 20L of selenium-enriched lotus leaf lyophyllum mycelia subjected to fermentation and culture by a biological tank as raw materials; optimizing the extraction process of selenium polysaccharides in the mycelia; analyzing the structure of the water-soluble selenium polysaccharides through infrared spectroscopy. The optimum extraction process conditions for obtaining the selenium polysaccharides through single-factor experiments and Box-Behnken test design method comprise the extraction time being 50min, the extraction temperature being 83 DEG C, the material-to-liquid ratio being 1:130g / mL, and the extraction time being twice. Through the measurement, the extraction rate of the selenium polysaccharides is 44.62 percent, and is increased by 0.02 percent in comparison with that of a prediction value. At the moment, the selenium content in the polysaccharides reaches 31.9 mug / g. The extraction rate is high. The purified polysaccharides are subjected to infrared spectroscopic analysis; the main body structure of the water-soluble selenium polysaccharides is not changed by rich selenium, but the absorption peak of pyranoid rings changes; the result shows that in the selenium-enriched culture process of the lotus leaf lyophyllum mycelia, the selenium participates in the synthesis of the selenium polysaccharides. The theoretical basis is provided for the development and the utilization ofthe selenium polysaccharides in the lotus leaf lyophyllum mycelia.

Description

Technical field [0001] The invention belongs to the field of biomedicine, and relates to the mycelium of the lotus leaf lycopersicum, in particular to a method for extracting and determining selenium polysaccharides from the selenium-enriched lotus leaf lycophorus mycelium. Background technique [0002] Lotus leaf pleated umbrella( Lyophyllum decastes (Fr.) Singer) is a fungus with high nutritional value and medicinal value. In addition, the mycelium of the lotus leaf has a certain selenium-enriching capacity. During the selenium-enriched fermentation process, the addition of selenium can effectively increase the dry weight of the mycelium. Selenium is an essential element for life activities and is closely related to human health. Studies have found that diseases such as anemia, Kashin-Beck disease, diabetes, and cancer are all related to the lack of selenium in the human body. From the analysis of geographical conditions, my country is a large selenium-deficient country, and n...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00G01N21/25G01N1/34G01N1/40C12N1/14C12P19/04C12R1/645
CPCC08B37/0003C12N1/14C12P19/04G01N1/34G01N1/4055G01N21/25
Inventor 高慧娟魏生龙席亚丽冯九海梁倩倩刘志芳
Owner HEXI UNIV
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