Single strand nucleic acid detection kit and method as well as application of single strand nucleic acid detection kit and method

A detection kit, single-stranded nucleic acid technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve the problems of increasing samples, insufficient signal strength, low signal amplification efficiency, etc., to reduce the possibility of contamination , The effect of improving signal amplification efficiency and high practical application value
CN107663530AActive Publication Date: 2018-02-06OCEAN UNIV OF CHINA
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
OCEAN UNIV OF CHINA
Publication Date
2018-02-06

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Abstract

The invention belongs to the technical field of molecular detection and particularly relates to a single strand nucleic acid detection kit and method as well as application of the single strand nucleic acid detection kit and method. The shown kit comprises cyclic dnazyme and a molecular beacon; the cyclic dnazyme has a dnazyme sequence. The kit can perform double cycle amplification on a fluorescence signal of the molecular beacon through simple and convenient operation of a one-step method under the assistance of RNase H so as to realize high-sensitivity specific detection on the single strand nucleic acid and is finally applied to detection of the single strand nucleic acid such as viruses or miRNA.
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Description

technical field

[0001] The invention relates to the technical field of molecular detection, in particular to a single-stranded nucleic acid detection kit, method and application thereof. Background technique

[0002] Nucleic acid detection methods have the advantages of high sensitivity, strong specificity and good stability, so they have been widely used in various research fields. Among them, nucleic acid signal amplification technology is a kind of detection technology that uses nucleic acid components to achieve optical or electrochemical signal enhancement in the system through amplification cycles. Nucleic acid signal amplification technology can realize highly sensitive detection of nucleic acid molecules, proteins, small molecular substances or ions. Therefore, it has been widely used in many fields such as food science, molecular biology and medicine in recent years.

[0003] At present, researchers have developed a variety of nucleic acid signal amplification tech...

Claims

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