Fusarium solani and method for producing dextranase by fermenting fusarium solani
A technology of Fusarium solani and dextranase, which is applied in the field of microbial application and can solve the problems such as dextranase that have not yet been produced
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Embodiment 1
[0027] Example 1 : Screening of strains
[0028] (1) Preliminary screening of plate medium: Accurately weigh 5g of soil samples from sewage outfalls of sugar factories, add 50ml of sterile water, place in a shaker at room temperature and shake for 30-60min at a shaking rate of 100rpm. After standing for 30 min, the supernatant was taken for serial dilution. Spread the gradient dilutions on the screening medium of the plate respectively, and culture them upside down at 28-37°C for 2-4 days.
[0029] (2) Select the bacterial strain that produces the hydrolysis circle from the screening plate in step (1), insert it into the fermentation medium and carry out the fermentation in the Erlenmeyer flask, and detect the enzyme activity after shaking culture at 28-37° C. and 180 rpm for 4-7 days.
[0030] Finally, a dextranase high-yielding strain strain DJ72 was obtained. This strain has been preserved in the General Microbiology Center of China Committee for Culture Collection of Mi...
Embodiment 2
[0031] Example 2 : Identification of strains
[0032] (1) Morphological analysis of strains
[0033] Such as figure 1 As shown, the colonies of the strain are neat, white or light yellow, fluffy, with septa and branched hyphae. Microconidia are spindle-shaped or oval, and macroconidia are fusiform or crescent-shaped.
[0034] (2) Molecular identification of the strain; the specific identification steps are as follows: extract the total DNA of the strain DJ72 and use it as a template, apply universal primers ITS1: 5'-TCCGTAGGTGAACCTGCGG-3' and ITS4: 5'-TCCTCCGC TTATTGATATG-3', PCR amplification The ITS was obtained, and a 521bp nucleotide sequence was obtained by sequencing, and its sequence was shown in SEQ ID NO.1.
[0035] The homology comparison analysis shows that it has the highest homology with the Fusarium solani strain, reaching 100%.
[0036] According to the results of morphological and molecular identification, DJ72 was identified as Fusarium solani.
Embodiment 3
[0037] Example 3 : The method for bacterial strain DJ72 fermentative production dextranase
[0038] (1) Seed culture: Transfer the bacterial strain from the plate screening medium to the liquid seed medium, and culture at 28°C and 150-200rpm for 2-3 days with shaking;
[0039] (2) Inoculate the seeds obtained in step 1 into a 50L fermenter with 35L fermentation medium at a volume ratio of 1-3%, at 28°C, cultivate for 6-7 days, control the stirring speed at 200-250rpm, and ferment the liquid The pH is controlled at 5.5.
[0040] During the fermentation process, 1 L of dextran T500 with a concentration of 10 g / L was fed at intervals of 12-24 hours as a feeding material.
[0041] After the fermentation, the final activity of dextranase was detected to be 124.3U / ml.
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