Preparation method of aquatic bacteriostatic agent

A bacteriostatic agent and aquatic product technology, applied in food ingredients as antimicrobial preservation, meat/fish preservation with chemicals, food science, etc., can solve the problems of poor reaction reproducibility, high energy consumption, and environmental hazards, and achieve High antibacterial activity, good synthesis reproducibility, and efficient reaction

Active Publication Date: 2018-02-13
YANTAI TUOPUBANG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, all three methods have certain limitations, such as high energ

Method used

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  • Preparation method of aquatic bacteriostatic agent
  • Preparation method of aquatic bacteriostatic agent
  • Preparation method of aquatic bacteriostatic agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1 Pyracantha fruit extract biosynthesis of nano-silver

[0028] a, preparation of Pyracantha fruit extract

[0029] Mature Pyracantha fruits were collected, rinsed with tap water, washed with distilled water three times, dried at 60°C to constant weight, ground into powder, and set aside.

[0030] Weigh 2 g of Pyracantha fruit powder, add 20 mL of 30% ethanol solution, and heat to reflux at 40° C. for 1.5 h. After natural cooling, suction filtration under reduced pressure, and centrifugation at 12000 rpm for 10 minutes, finally obtain the ethanol extract of Pyracantha japonicus, which is stored at 4°C for future use.

[0031] b. Biosynthesis of silver nanoparticles

[0032] Take Pyracantha fruit extract 1mL, add 0.01M AgNO 3 Solution 1mL, then add double distilled water 18mL, after stirring evenly, ultrasonic treatment at 100, 200, 400, 800W respectively for 1h, the reaction temperature is controlled at 50°C.

[0033] c, UV-visible spectrophotometer detec...

Embodiment 2

[0035] Example 2 Pyracantha fruit extract biosynthesis of nano-silver

[0036] a, preparation of Pyracantha fruit extract

[0037] Mature Pyracantha fruits were collected, rinsed with tap water, washed with distilled water three times, dried at 60°C to constant weight, ground into powder, and set aside.

[0038] Weigh 2 g of Pyracantha fruit powder, add 20 mL of 100% ethanol solution, and heat to reflux at 90° C. for 30 min. After natural cooling, suction filtration under reduced pressure, and centrifugation at 12000 rpm for 10 minutes, finally obtain the ethanol extract of Pyracantha japonicus, which is stored at 4°C for future use.

[0039] b. Biosynthesis of silver nanoparticles

[0040] Take Pyracantha fruit extract 1mL, add 0.1M AgNO 3 Solution 2mL, then add double distilled water 17mL, stir evenly, 600W ultrasonic treatment 0min, 10min, 20min, 30min, 40min, 60min, reaction control temperature 90℃.

[0041] c, UV-visible spectrophotometer detection of nano-silver

[...

Embodiment 3

[0043] Example 3 Pyracantha fruit extract biosynthesis of nano-silver

[0044] a, preparation of Pyracantha fruit extract

[0045] Mature Pyracantha fruits were collected, rinsed with tap water, washed with distilled water three times, dried at 60°C to constant weight, ground into powder, and set aside.

[0046] Weigh 2 g of Pyracantha fruit powder, add 20 mL of 60% ethanol solution, and heat to reflux at 70° C. for 1 h. After natural cooling, suction filtration under reduced pressure, and centrifugation at 12000 rpm for 10 minutes, finally obtain the ethanol extract of Pyracantha japonicus, which is stored at 4°C for future use.

[0047] b. Biosynthesis of silver nanoparticles

[0048] Take Pyracantha fruit extract 1mL, add 0.01M AgNO 3 Solution 2mL, then add double distilled water 17mL, stir evenly, 600W ultrasonic treatment for 1h, reaction control temperature 75°C.

[0049] c. Transmission electron microscope characterization detection

[0050] Get the biosynthetic nano...

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Abstract

The invention discloses a preparation method of an aquatic bacteriostatic agent and belongs to the field of aquaculture. The method comprises the following steps: collecting ripe pyracantha crenatoserrata fruits, cleaning, drying and grinding into powder; adding an ethanol solution with a volume ratio of 30-100% into the pyracantha crenatoserrata fruit powder; heating and refluxing; cooling naturally; performing reduced-pressure suction filtration; centrifuging to obtain pyracantha crenatoserrata ethanol extract; adding a AgNO3 solution into the pyracantha crenatoserrata ethanol extract; performing ultrasonic treatment on the reaction liquid in an ultrasonic apparatus; ending the reaction when the solution turns golden yellow, so as to obtain a biologically synthesized nano silver bacteriostatic agent. According to the method disclosed by the invention, the nanosiliver bacteriostatic agent is prepared in onestep by combining the heating and ultrasonic radiation technologies and using the effect of biologically synthesizing nano silver from pyracantha crenatoserrata fruit extract, and has the advantages of easiness in operation, efficient reaction, high synthesis reproducibility, uniform and stable product, high bacteriostatic activity, and the like.

Description

technical field [0001] The invention belongs to the field of aquaculture, and specifically relates to a preparation method of an aquatic antibacterial agent. Background technique [0002] In recent years, due to the continuous expansion of global aquaculture scale and aquaculture density and the deterioration of water pollution, aquatic diseases have continued to occur and spread, seriously affecting the sustainable development of aquaculture. At present, people generally use chlorine, ozone, chlorine dioxide, bleaching powder and other disinfectants as well as sulfonamides, quinolones, antibiotics and other antibacterial agents to control the occurrence of aquatic diseases. Poor effect, drug residue, pollution of water environment and other shortcomings. In addition, long-term use of antibiotics will also cause pathogenic bacteria to develop resistance and antagonism to the drug, and eventually lead to the loss of bactericidal effect of the drug and be eliminated. Therefo...

Claims

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Application Information

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IPC IPC(8): A23B4/24
CPCA23B4/24A23V2002/00A23V2200/10A23V2250/1562
Inventor 王磊宿红艳梁徐健刘夏宇戴昕彤徐慧
Owner YANTAI TUOPUBANG BIOTECH
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