Culture medium for regeneration of somatic embryogenesis way of nelumbo nucifera and application of culture medium

Abstract

The invention provides a culture medium for regeneration of a somatic embryogenesis way of nelumbo nucifera and an application of the culture medium. The culture medium comprises an embryonic callus induction and proliferation culture medium and an embryoid generation culture medium. A culture method comprises the steps as follows: an explant is placed in the embryonic callus induction and proliferation culture medium for culture, and callus is formed through induction and subjected to proliferation culture; a callus block is transferred to the embryoid generation culture medium to develop into an embryoid; the produced embryoid is transferred to an embryo germination culture medium to germinate cotyledonary embryo; and the germinated cotyledonary embryo is transferred to a nelumbo nucifera seedling culture medium for seedling culture, and cultured seedlings are transplanted to the natural environment. With the adoption of the culture medium for culture, one explant can differentiate an embryonic callus group which can efficiently differentiate 1,000 finally germinating somatic embryos or more, the transgenosis development of the nelumbo nucifera can be promoted, and a large quantity of seedlings can be provided for cultivation and application of the nelumbo nucifera.

Description

technical field [0001] The invention belongs to the technical field of tissue culture, and in particular relates to a plant tissue culture system, in particular to a regeneration medium for lotus somatic embryogenesis pathway and application thereof. Background technique [0002] Lotus (Nelumbonucifera Gaertn.) is a perennial aquatic flower of the genus Nelumbonaceae. It is one of the top ten traditional famous flowers in China. It has high ornamental value, economic value and unique cultural connotation, and is deeply loved by people. According to its use, it can be divided into lotus root, seed lotus and flower lotus. [0003] In 2013, the complete genome sequencing of the lotus variety - the ancient Chinese lotus was completed, laying a good foundation for the study of the gene function of the lotus. However, the genetic transformation and regeneration system of lotus, which is an important technical basis for transgenics, is still immature, which seriously restricts the...

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Problems solved by technology

[0006] In the prior art, when lotus was developed as a commercial product, the reproduction coefficient and cost of artificial breeding of lotus root were low and the cost was high; at the same time, there was a lack of efficient regeneration system in basic research, which limited the development of genetic engineering breeding work; these factors were unfavorable for the development of lotus. Economic cultivation and genetic breeding work

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