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A kind of method for cultivating subcardiac flat algae

A sub-cardioid, flat algae technology, applied in the field of algae culture, can solve the problems of slow growth of algal cells and high cost of sub-cardioid flat algae culture medium, achieve the effects of fast proliferation, promote algal cell proliferation, and increase supply

Active Publication Date: 2020-11-03
杭州富阳优信科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the defects of the prior art such as the high cost of the subcardiac culture solution and the slow growth of algae cells, and to provide a method for cultivating the subcardiac

Method used

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  • A kind of method for cultivating subcardiac flat algae
  • A kind of method for cultivating subcardiac flat algae

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A method for cultivating subcardiac flat algae, comprising the steps of:

[0029] Take the subcardiac flat algae in the logarithmic growth phase, inoculate 7% of the inoculum into the container containing the culture medium for cultivation, the light intensity is 6000lx, the light-to-dark ratio is 12:12, the water temperature is 25-26°C, and the pH is adjusted The value was 7.5, cultured for 8 days.

[0030] The culture solution is prepared according to the following process: take vitamin B6 0.1mg, vitamin B12 0.1mg, cobalt chloride hexahydrate 30mg, sodium selenite 40mg, iron citrate 50mg, magnesium sulfate heptahydrate 100mg, zinc sulfate heptahydrate 200mg, wheat bran extract 20ml, rice straw extract 50ml, the balance is water, dilute to 1L.

[0031] The wheat bran extract is prepared according to the following process:

[0032] Grind the wheat bran and place it in a reaction tank, add 2 times the weight of 6M hydrochloric acid, stir and hydrolyze at 60°C for 8 hou...

Embodiment 2

[0036] A method for cultivating subcardiac flat algae, comprising the steps of:

[0037] Take the subcardiac flat algae in the logarithmic growth phase, inoculate 6% of the inoculum into the container containing the culture medium for cultivation, the light intensity is 5000lx, the light-to-dark ratio is 12:12, the water temperature is 25°C, and the pH value is adjusted to 7.7 , cultured for 6 days.

[0038] The culture solution is prepared according to the following process: take vitamin B6 0.2mg, vitamin B12 0.2mg, cobalt chloride hexahydrate 20mg, sodium selenite 30mg, iron citrate 70mg, magnesium sulfate heptahydrate 150mg, zinc sulfate heptahydrate 150mg, wheat bran extract 20ml, wheat straw extract 40ml, the balance is water, dilute to 1L.

[0039] The wheat bran extract is prepared according to the following process:

[0040] Grind the wheat bran and put it in a reaction tank, add 2 times the weight of 5M hydrochloric acid, stir and hydrolyze at 55-60°C for 8 hours at...

Embodiment 3

[0044] A method for cultivating subcardiac flat algae, comprising the steps of:

[0045] Take the subcardiac flat algae in the logarithmic growth phase, inoculate 5% of the inoculum into the container containing the culture medium for cultivation, the light intensity is 10000lx, the light-to-dark ratio is 12:12, the water temperature is 25°C, and the pH value is adjusted to 7.8 , cultured for 7 days.

[0046] The culture solution is prepared according to the following process: take vitamin B6 0.1mg, vitamin B12 0.1mg, cobalt chloride hexahydrate 20mg, sodium selenite 20mg, iron citrate 50mg, magnesium sulfate heptahydrate 100mg, zinc sulfate heptahydrate 150mg, 40ml of wheat bran extract, 40ml of corn stalk extract, and water as the balance, dilute to 1L.

[0047] The wheat bran extract is prepared according to the following process:

[0048] Grind the wheat bran and place it in a reaction tank, add 2 times the weight of 5M hydrochloric acid, stir and hydrolyze at 57°C for 1...

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Abstract

The invention belongs to the technical field of algae culture, and discloses a method for culturing marine microalga. The method comprises the following steps: taking the marine microalga in a logarithmic growth period, inoculating a container containing a culture solution with the 5%-7% marine microalga according to the inoculation amount, controlling light intensity to be 5000-10000 lx, light todark ratio to be 12:12, water temperature to be 25-26 DEG C and pH to be 7.5-8, and culturing the marine microalga for 6-8 days. The culture method is simple and implementable, the algae cell proliferation speed is high, and the cost is low.

Description

technical field [0001] The invention belongs to the technical field of algae cultivation, and in particular relates to a method for cultivating subcardiac flat algae. Background technique [0002] Subcardiac flat alga (Marine microalga), belongs to Volvox, Chlamydomonas, algae. Latin name Platymonas subcordiformis, distributed in oceans, coastal ponds and calmer harbors. Subcardiac Pyramides can adapt to a wide range of salinity, and can grow and reproduce in seawater with a salinity of 8-80‰. The optimum salinity range is 30-40‰, and the temperature adaptation range is also very wide. It can grow and reproduce in the range of 7°C-30°C. The optimum temperature is about 20°C-28°C, and the temperature rises above 33°C The growth and reproduction are greatly inhibited, and the yellowish state appears. When the temperature is close to 35°C, a large number of flat algae will die, so it is difficult to cultivate in the south in summer; The optimum light intensity is about 5000-...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/38C12N1/12C12R1/89
CPCC12N1/12C12N1/38
Inventor 陈金华吕兰婷吕伟红陈国汀吕伟德黄土根
Owner 杭州富阳优信科技有限公司