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Molecular marker miR-452 for early diagnosing acute kidney injury caused by septicopyemia, kit and application thereof

A technology of acute kidney injury and molecular markers, applied in biochemical equipment and methods, microbiological measurement/testing, DNA/RNA fragments, etc., can solve the problems of difficult early diagnosis, specific detection, and impossibility, and achieve improvement Early detection rate, improved specificity, improved treatment and prognosis

Active Publication Date: 2018-02-16
THE SECOND XIANGYA HOSPITAL OF CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

At present, the understanding of the pathogenesis of sepsis AKI is very limited, and it is difficult to diagnose early clinically
Although serum creatinine and blood urea nitrogen are the most commonly used indicators for evaluating renal function, their use for the diagnosis and monitoring of sepsis AKI still has great limitations, especially the early, sensitive and specific detection cannot be carried out

Method used

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  • Molecular marker miR-452 for early diagnosing acute kidney injury caused by septicopyemia, kit and application thereof
  • Molecular marker miR-452 for early diagnosing acute kidney injury caused by septicopyemia, kit and application thereof
  • Molecular marker miR-452 for early diagnosing acute kidney injury caused by septicopyemia, kit and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Gene chip analysis: AKI induced by intraperitoneal injection of lipopolysaccharide (LPS) in mice with sepsis, and microRNA expression profile changes in kidney tissue were analyzed by chip technology. The results showed that after 24 hours of LPS treatment, the expression of some microRNAs, including miR-452, was up-regulated. These results reveal the possible changing trend of microRNA in sepsis AKI and lay the foundation for the next experiments.

[0024] Animal experiment design:

[0025] C57BL / 6 mice, 6-8 weeks old, were purchased from Changsha Slake Jingda Experimental Animal Co., Ltd. We induced sepsis animal model by intraperitoneal injection (dose 10 mg / kg) of LPS (purchased from Sigma, USA). The specific grouping is as follows in Table 1: (LPS is dissolved in normal saline, and the control (control group) is simply injected with normal saline)

[0026] Table 1

[0027]

[0028] After the injection, the mice were put back into the breeding cage and provid...

Embodiment 2

[0035] In addition to animal experiments, clinical specimens were collected at the same time, and relevant clinical tests and tests were carried out. A total of 36 blood and urine samples from patients with sepsis were collected in the Second Xiangya Hospital of Central South University; inclusion criteria: 1) meet the diagnostic criteria for sepsis, and 2) have no history of chronic kidney disease or other kidney diseases. At the same time, 18 healthy control specimens were collected from the Physical Examination Center of the Second Xiangya Hospital of Central South University.

[0036] Total RNA in blood and urine samples was extracted from the obtained samples, and miR-452 was measured by TaqMan-based Real-time-PCR (the specific method is the same as that of mouse serum). It was found that compared with healthy subjects: the expression of miR-452 in urine and serum of patients with sepsis was up-regulated, such as image 3 shown.

Embodiment 3

[0038] In order to explore whether there is a certain correlation between the serum creatinine value and the expression of miR-452 in blood and urine in patients with sepsis, the correlation analysis between serum creatinine and the expression of miR-452 in blood and urine was carried out. The results are as follows: Figure 4 shown. The results showed that there was a correlation between the serum creatinine value and the expression of blood miR-452 and urinary miR-452, both of which were statistically significant, p2 = 0.7077.

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Abstract

The invention discloses a molecular marker miR-452 for early diagnosing acute kidney injury caused by septicopyemia, a kit and an application thereof. The molecular marker is used for early diagnosingof acute kidney injury caused by septicopyemia. In the body of a septicopyemia patient, the blood and urine miR-452 expressions are both up-regulated and both have certain relevance with serum creatinine but the relevance in urine is better, so that miR-452 expression up-regulation is possibly related to the renal injury. A diagnostic test shows that pure screening for urine miR-452 molecule is higher in sensitivity for diagnosing septicopyemia AKI but the combined screening for blood and urine miR-452 is capable of promoting the specificity of diagnosis. The result of the invention proves that miR-452 can be used as a biomarker for diagnosing septicopyemia AKI and has the advantage of early diagnosis and higher sensitivity and specificity.

Description

technical field [0001] The invention relates to the technical field of molecular diagnosis, in particular to a molecular marker, kit and application for early diagnosis and prediction of acute kidney injury caused by sepsis. Background technique [0002] Acute kidney injury (acute kidney injury, AKI), also known as acute renal failure, is a rapid decline or even loss of kidney function caused by a variety of reasons, resulting in the body not being able to excrete various toxins and metabolites in the body in time, which in turn causes various Harmful substances or normal metabolites in the body accumulate in the body, causing disturbance of the internal environment and abnormal metabolism of the body, and symptoms of various systems of the body appear. There are many causes of AKI, such as the use of nephrotoxic drugs, renal ischemia-reperfusion injury, systemic inflammatory response, sepsis, severe liver disease, and insufficient circulating blood volume. [0003] Clinica...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12N15/113
CPCC12N15/113C12N2310/14C12Q1/6883C12Q2600/158C12Q2600/178
Inventor 董政刘志文杨淡昳
Owner THE SECOND XIANGYA HOSPITAL OF CENT SOUTH UNIV
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