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Method for producing nosiheptide by fermentation of streptomyces actuosus

A technology of active streptomyces and nosiheptide, applied in the field of microbial production of nosiheptide, can solve the problems of low yield and high production cost, achieve the effect of improving biological activity, increasing yield, and ensuring normal production and metabolism

Inactive Publication Date: 2018-02-23
黄增才
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the existing microbial fermentation method produces nosiheptide, which has high production cost and low yield.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The source of active Streptomyces used in this example is commercially available.

[0019] Prepare media according to the following recipes: slant media, seed pot media, and fermenter media.

[0020] Slant medium: soluble starch 2.1g / 100mL, K 2 HPO 4 0.08g / 100mL, MgSO 4 0.06g / 100mL, FeSO 4 0.003g / 100mL, NaCl0.03g / 100mL, KNO 3 0.02 g / 100mL, agar 2.0 g / 100mL;

[0021] Seed tank medium: glucose 2 g / 100mL, soybean cake powder 2.5 g / 100mL, (NH 4 ) 2 SO 4 0.2g / 100mL, MgSO 4 0.03 g / 100mL, calcium carbonate CaCO 3 0.4 g / 100mL, K 2 HPO 4 0.02 g / 100mL, soybean oil 0.3 g / 100mL, defoamer 0.02 g / 100mL;

[0022] Fermenter medium: soluble starch 6.0 g / 100mL, soybean cake powder 3.5 g / 100mL, (NH 4 ) 2 SO 4 1.0g / 100mL, CaCO 3 1.0 g / 100mL, K 2 HPO 4 0.08 g / 100mL, NaCl 0.10 g / 100mL, soybean oil 0.8 g / 100mL, defoamer 0.05 g / 100mL.

[0023] Use a sterile inoculation needle to dig an appropriate amount of sand and inoculate it on the slant medium for 8 days, add sterile w...

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Abstract

The invention discloses a method for producing nosiheptide by fermentation of streptomyces actuosus. The method comprises the following steps of: carrying out microspore culture on a slant culture medium, carrying out strain culture on a seed-tank culture medium, carrying out fermentation on a fermentation-tank culture medium, carrying out optimization on culture or fermentation parameters in allthe steps. Compared with the prior art, the method disclosed by the invention has the beneficial effects that the activation of the streptomyces actuosus is optimized, and the biological activity of the streptomyces actuosus is improved by culture at different stages; the yield of the nosiheptide is increased by 60% or more.

Description

technical field [0001] The invention belongs to the field of microbial fermentation and relates to a microbial production method of nosiheptide. Background technique [0002] Nosiheptide is a polypeptide antibiotic with 5 wah rings, its molecular formula is C51H43N13O12S6, and its molecular weight is 1222. Nosiheptide is a yellow-green substance in the form of powder or fine needle crystals, with a melting point of 310-320°C (with decomposition). Nosiheptide is soluble in chloroform, pyridine, dimethylamine, dimethylsulfoxide, slightly soluble in methanol, ethanol, ethyl acetate and benzene, and insoluble in water. With its relative safety and good application effect, nosiheptide meets the current people's requirements for feed additives, and is an ideal substitute for feed antibiotics. Currently, it has been approved and widely used in the European Union and Japan. [0003] Nosiheptide is produced by microbial culture method, and nosiheptide-producing bacteria mainly inc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/02C12N1/20C12R1/465
CPCC12P21/02C07K5/06139C12N1/20
Inventor 黄增才
Owner 黄增才
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