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A kind of technique of extracting collagen from fish scale

A technology of collagen and fish scales, which is applied to animal/human proteins, methods based on microorganisms, biochemical equipment and methods, etc., can solve the problems of low protein purity, affecting product quality, etc., and achieve collagen peptides with small molecular weight and low ash content , the effect of high yield

Inactive Publication Date: 2019-04-23
SOUTH CHINA NORMAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The above two extraction methods are just simple acid-base or cooking treatment, but the network structure has formed between hydroxyapatite, protein and other substances in fish scales, and simple acid-base treatment cannot remove hydroxyapatite, etc. Other substances directly lead to low protein purity and affect product quality

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  • A kind of technique of extracting collagen from fish scale
  • A kind of technique of extracting collagen from fish scale

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Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1 A kind of technique of extracting collagen from fish scale

[0029] Described processing step is:

[0030] S1 demineralization: wash, dry and pulverize the fish scales to a particle size of 180 mesh, add it to a mixed acid solution of 45 times the mass, react with ultrasonic waves for 45 minutes, filter, wash with water until the pH value of the filtrate reaches 6.7, and dry the filter residue to obtain Acid hydrolyzed fish scale powder A;

[0031] S2 Degreasing: add the acid hydrolyzed fish scale powder A obtained in step S1 into a nonionic surfactant solution with a mass fraction of 6%, react with ultrasonic waves for 4 hours, filter, put the filter residue into a sodium hydroxide solution with a mass fraction of 0.7%, and heat at 30°C Stand and soak for 10 hours, filter, and wash with water until the pH value of the filtrate reaches 7.3, and dry the filter residue to obtain hydrolyzed fish scale powder B; wherein, the material ratio of acid hydrolyzed f...

Embodiment 2

[0037] Embodiment 2 A kind of technique of extracting collagen from fish scale

[0038] Described processing step is:

[0039] S1 demineralization: wash, dry and pulverize the fish scales to a particle size of 150 mesh, add it to a mixed acid solution of 40 times the mass, react with ultrasonic waves for 40 minutes, filter, and wash with water until the pH value of the filtrate reaches 6.5, and dry the filter residue to obtain Acid hydrolyzed fish scale powder A;

[0040] S2 degreasing: add the acid hydrolyzed fish scale powder A obtained in step S1 into a nonionic surfactant solution with a mass fraction of 5%, react with ultrasonic waves for 3 hours, filter, put the filter residue into a sodium hydroxide solution with a mass fraction of 0.5%, and keep at 25°C Stand and soak for 8 hours, filter, and wash with water until the pH value of the filtrate reaches 7.2, and dry the filter residue to obtain hydrolyzed fish scale powder B; wherein, the material ratio of acid hydrolyze...

Embodiment 3

[0046] Embodiment 3 A kind of technique of extracting collagen from fish scale

[0047] Described processing step is:

[0048] S1 demineralization: wash, dry and pulverize the fish scales to a particle size of 200 mesh, add it to a mixed acid solution with 50 times the mass, react with ultrasonic waves for 50 minutes, filter, wash with water until the pH value of the filtrate reaches 6.8, and dry the filter residue to obtain Acid hydrolyzed fish scale powder A;

[0049] S2 Degreasing: add the acid hydrolyzed fish scale powder A obtained in step S1 into a nonionic surfactant solution with a mass fraction of 7%, react with ultrasonic waves for 5 hours, filter, put the filter residue in a sodium hydroxide solution with a mass fraction of 1%, and heat at 35°C Stand and soak for 12 hours, filter, and wash with water until the pH value of the filtrate reaches 7.5, and dry the filter residue to obtain hydrolyzed fish scale powder B; wherein, the material ratio of acid hydrolyzed fis...

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Abstract

The invention belongs to the technical field of biological extraction, and specifically relates to a process for extracting collagen from fish scales, comprising the following steps: S1 demineralization: acid hydrolysis of fish scales; S2 degreasing: combined treatment with surfactant and alkali; S3 cooking; S4 Expansion treatment; S5 fermentation: Protease-producing strains are cultivated to decompose proteins; the process of the present invention is simple, which solves the problems of extracting collagen with many impurities and low yield in the prior art, and the extracted collagen has a small molecular weight, and the obtained The collagen protein has high yield and high purity, and the fish meat obtained by applying it to fish feed is of good quality.

Description

technical field [0001] The invention belongs to the technical field of biological extraction, and in particular relates to a technique for extracting collagen from fish scales. Background technique [0002] In recent years, the fish product industry has become more and more extensive. Most of the freshwater fish processing process is limited to the use of fish body muscles, while the processing and utilization of wastes such as fish scales are seldom used. Fish scales are the tissues that the fish body contacts with the outside world. It is a deformation of the dermis of fish, usually accounting for 1% to 5% of the weight of the fish, and plays a role in protecting the fish from injury. Fish scales are mainly composed of protein, lecithin, hydroxyapatite and guanine, of which organic matters account for 41%-55%, and calcium salts 38%-46%. Among them, fish scales are rich in protein components, mainly collagen and fish scale horns Protein, if fish scales are used, can effect...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P39/00C12P21/02C12R1/125C12R1/865
CPCC07K14/78C12P39/00
Inventor 潘淦许爱娱
Owner SOUTH CHINA NORMAL UNIVERSITY