A thin-layer chromatography identification method for identifying snow gall varieties

Abstract

The invention provides a thin layer chromatography identification method for identifying the variety of Hemsleya chinensis. The thin layer chromatography identification method comprises: (1) preparinga test solution: taking the powder of a herb to be detected, adding methanol, extracting, filtering, and concentrating to obtain a test solution; (2) preparing a reference substance solution: takinga cucurbitacin IIa reference substance and a cucurbitacin IIb reference substance, mixing, and adding methanol to prepare a mixing reference substance solution; and (3) carrying out thin layer chromatography identification: respectively spotting the reference substance solution and the test solution on a thin layer plate, developing with a developing solvent, coloring, inspecting, and comparing, wherein the developing solvent comprises, by volume, 4-6 parts of petroleum ether, 0.5-1.5 parts of ethyl acetate, and 0.1-1 part of methanol. According to the present invention, the non-bitter Hemsleya omeiensis L.T.Shen et W.J.Chang can be accurately and reliably identified, and can be distinguished from the bitter Hemsleya chinensis (hemsleya dolichocarpa w.j.chang and Hemsleya gigantha W.J.Chang) so as to provide the effective assurance for the clinical medication.

Description

technical field [0001] The invention belongs to the field of medicinal material detection, and in particular relates to a thin-layer chromatographic identification method for identifying snow gall varieties. Background technique [0002] The snow gall contained in Chuanbiao is the dry tuber root of Hemsleya dolichocarpa W.J.Chang, Hemsleya omeiensis L.T.Shen et W.J.Chang or Hemsleyagigantha W.J.Chang, a plant of the genus Cucurbitaceae Snow gall. Excavated in late autumn and early spring, removed impurities, sliced ​​while fresh, and dried. Also known as the golden tortoise lotus, the bottom of the pot, it is commonly called the golden tortoise lotus in Sichuan Province. It can clear away heat and detoxify, reduce inflammation and relieve pain, and is used for bacillary dysentery, enteritis, bronchitis, acute tonsillitis, toothache due to deficiency fire, and stomach pain. This product has been recorded in successive dynasties of herbal medicines. "Materia Medica Recipe" ...

AI Technical Summary

Problems solved by technology

[0004] However, due to the lack of current research on snow gall, many active ingredients or characteristic ingredients are unknown, so there is a lack of quantifiable treatment evaluation methods and standards, and it is difficult to effectively distinguish the bitter snow gall, especially after drying. herbs

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