A method for detecting an iron ion concentration of a solution by utilizing nitrogen-doped carbon quantum dots
A technology of iron ion concentration and carbon quantum dots, applied in measurement devices, instruments, fluorescence/phosphorescence, etc., can solve the problems of high test cost, complicated operation, long time consumption, etc., and achieve simple and convenient detection process, high sensitivity and good effect. Effect
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Embodiment 1
[0034] A method utilizing nitrogen-doped carbon quantum dots to detect the concentration of iron ions in a solution, comprising the following steps:
[0035] Step 1. To prepare nitrogen-doped carbon quantum dots, first add 2.5g of trypsin to 10ml of ultrapure water and mix and stir for 5 minutes to obtain a mixed solution, then transfer the prepared mixed solution to a 25ml reaction kettle for water treatment at 180°C Thermal reaction for 12 hours to obtain the nitrogen-doped carbon quantum dot crude liquid, the obtained nitrogen-doped carbon quantum dot crude liquid was centrifuged at a speed of 6000 rad / min to obtain the supernatant for filtration, filtered with a 0.22 μm filter to obtain the filtrate, and then the filtrate was used A dialysis bag with a molecular weight of 1000Da was dialyzed to obtain a dialysate, and the dialysate was vacuum-dried at 60°C for 24 hours to obtain water-soluble yellow fluorescent nitrogen-doped carbon quantum dots, and finally the obtained ni...
Embodiment 2
[0045] A method for detecting the concentration of iron ions in a solution by using nitrogen-doped carbon quantum dots, the process of which is roughly the same as in Example 1, the difference being that: when preparing nitrogen-doped carbon quantum dots in step 1, trypsin is added to ultrapure water After the mixed solution is prepared, the mixed solution is ultrasonically dispersed, the ultrasonic frequency is 28KHz, and the time is 20min, and then the ultrasonically treated mixed solution is subjected to infrared heating treatment, the heating rate is 2°C / min, and the final temperature is 54°C. Carry out the vacuum distillation operation in the middle, the air pressure decreases gradually at the speed of 250Pa / min, when the heating process ends, the air pressure also stops decreasing, maintain the temperature and air pressure conditions for 30min, and then raise the temperature to 78°C at a heating rate of 3°C / min °C, the air pressure decreases gradually at a rate of 400Pa / m...
Embodiment 3
[0048] A method for detecting the concentration of iron ions in a solution by using nitrogen-doped carbon quantum dots, the process of which is roughly the same as in Example 1, the difference being that: when preparing nitrogen-doped carbon quantum dots in step 1, trypsin is added to ultrapure water After the mixed solution is prepared, the mixed solution is ultrasonically dispersed, the ultrasonic frequency is 33KHz, and the time is 35min, and then the ultrasonically treated mixed solution is subjected to infrared heating treatment, the heating rate is 2°C / min, and the final temperature is 58°C. Carry out the vacuum distillation operation in the middle, the air pressure decreases gradually at the speed of 250Pa / min, when the heating process ends, the air pressure also stops decreasing, maintain the temperature and air pressure conditions for 30min, and then raise the temperature to 84°C at a heating rate of 3°C / min °C, the air pressure decreases gradually at a rate of 400Pa / m...
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