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A strain of Bacillus pumilus s9 for controlling rice blast

A technology for Bacillus pumilus and rice blast, which is applied in the field of microorganisms to achieve broad-spectrum control effects, high control efficiency, and good yield-promoting effects.

Active Publication Date: 2019-11-29
INST OF PLANT PROTECTION NINGXIA ACAD OF AGRI & FORESTRY SCI KEY LAB OF NINGXIA PLANT DISEASE & INSECT PESTS CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The research and application of Bacillus to control rice blast is the need for sustainable development of rice production, and can meet the requirements of the United Nations World Food Organization for the development of food security. Bacillus strains with high production of active substances

Method used

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  • A strain of Bacillus pumilus s9 for controlling rice blast
  • A strain of Bacillus pumilus s9 for controlling rice blast
  • A strain of Bacillus pumilus s9 for controlling rice blast

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1: Screening and fermentation culture of Bacillus pumilus S9 bacterial strain

[0029] (1) Sample collection: The sample was collected from the rhizosphere soil of rice variety Ningjing 48 at Yaofu Town, Pingluo County, Ningxia Hui Autonomous Region. Pull out together with the root system of rice, pack it in a polyethylene plastic bag, and bring it back to the laboratory for isolation.

[0030] (2) Isolation of Bacillus pumilus: Weigh 1 g of soil, put it into 100 mL of sterile water, shake and mix at room temperature for 1 hour, and use sterile water according to 10, 10 2 and 10 3 For gradient dilution, take 200mL / dish of the gradient dilution with a micropipette and spread it on the NA solid medium plate with a spreader stick. After culturing in a 37°C incubator for 48 hours, pick up a single colony on the NA solid medium plate with an inoculation needle. Stretch with a toothpick gradient to obtain a single bacterial colony after purification, and store th...

Embodiment 2

[0037] Embodiment 2: the biological activity assay of bacillus pumilus S9 bacterial strain:

[0038] (1) Bacteriostatic determination of Bacillus pumilus S9 strain on Pyricularia grisea: Place Pyricularia oryzae P131 bacterial cake in the center of the tomato oat plate medium, and inoculate single colony Bacillus bacterial cake at a distance of 2cm from both sides of the bacterial cake after 4 days of growth For confrontation culture, use sterile water as a control, and place in an incubator at 28°C for dark culture. Each treatment was repeated 3 times. After 2 days, the diameter of the pathogenic bacteria colony and the zone of inhibition were measured, and the results are shown in Table 1.

[0039] (2) Bacillus pumilus S9 aseptic supernatant is to the bacteriostasis determination of rice blast bacterium: Place the rice blast fungus bacterium cake in the center on the tomato oat plate culture medium, place aseptic Oxford cup at the 2cm place apart from the bacteria cake both...

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Abstract

The invention discloses a Bacillus pumilus for controlling rice blast and application thereof. The bacillus pumilus of the invention has broad-spectrum and high-efficiency control effects on rice blast, and can be used for preventing and controlling rice blast of various strains of rice, especially leaf blast and panicle blast.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a strain of Bacillus pumilus for controlling rice blast and its application. Background technique [0002] Rice is an important food crop in the world. Its output accounts for more than 1 / 4 of the world's total food production, more than half of China's total food production, and is the staple food that more than half of the world's population depends on. Rice blast (Rice Blast) is a kind of sudden and strong worldwide fungal disease caused by Ascomycete Magnaporthe oryzae (T.T.Hebert) Yaegashi&Udagawa (asexual state: Pyricularia oryzae). The disease can cause harm to rice all year round, and its harm spreads all over the various parts of rice, including seedling rice blast, leaf blast, leaf pillow blast, node rice blast, ear neck blast, branch blast and grain blast. Rice blast is widely distributed and occurs in 85 countries around the world. It is also one of the most serious ric...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/07
CPCA01N63/00C12N1/20C12N1/205C12R2001/07
Inventor 沙月霞王昕沈瑞清刘炜石东岭方秋香
Owner INST OF PLANT PROTECTION NINGXIA ACAD OF AGRI & FORESTRY SCI KEY LAB OF NINGXIA PLANT DISEASE & INSECT PESTS CONTROL
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