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Applications of nanometer needle electrode in ultrahigh density electrochemical sensing analysis

An ultra-high-density, nano-needle technology, applied in the field of chemical analysis, can solve problems affecting the accuracy of electrochemical quantification and achieve good reproducibility

Active Publication Date: 2018-03-16
NANJING UNIV
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Problems solved by technology

However, the intermediates produced during ECL have relatively long lifetimes (~microseconds) and large diffusion distances (~micrometers) leading to overlapping of optical signals in adjacent regions, thereby affecting the accuracy of electrochemical quantification10

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  • Applications of nanometer needle electrode in ultrahigh density electrochemical sensing analysis
  • Applications of nanometer needle electrode in ultrahigh density electrochemical sensing analysis
  • Applications of nanometer needle electrode in ultrahigh density electrochemical sensing analysis

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Embodiment Construction

[0022] The invention can be better understood from the following examples. However, those skilled in the art will easily understand that the content described in the embodiments is only for illustrating the present invention, and shall not and will not limit the present invention described in the claims.

[0023] Fabrication of nanoneedle electrode arrays and ECL imaging.

[0024] (1) Fabrication of nanoneedle electrode arrays.

[0025] Nanoneedle electrode arrays were prepared according to the methods reported in the literature, such as Figure 9 shown. (Alves, M.A.R.; Takeuti, D.F.; Braga, E.S. Microelectronics J. 2005, 36, 51–54; Han, J.; Lu, S.; Li, Q.; Li, X.; Wang, J.; Sens. Actuators A Phys.2009, 152, 75–79.), 4-inch, p-type, silicon wafer, resistivity 1-20 Ω cm, thickness 500 mm, cleaned with standard RCA and thermally oxidized to produce 200 nm of SiO 2 layer. Photolithographic templates are generated by photolithography and transferred to SiO by reactive ion et...

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Abstract

The invention discloses applications of a nanometer needle electrode in ultrahigh density electrochemical sensing analysis. In the application, a nanometer needle electrode array is used as a workingelectrode, a Ag / AgCl electrode is used as a reference electrode, a Pt wire is used as an auxiliary electrode, an ECL system achieves a local ECL signal at the tip of a nanometer needle by using a voltage conversion mode, and a ultrahigh density electrochemical experiment target molecule is quantitated by using the light emitting intensity of the tip of the nanometer needle, wherein the electrode density of the nanometer needle electrode array is 1.6*10<3>4*10<3>, the diameter of the tip of the nanometer needle is 700-750 nm, the diameter of the bottom portion of the tip is 1-4 [mu]m, and the height of the nanometer needle is 2-8 [mu]m. According to the present invention, the applications of the nanometer needle electrode with the local ECL as the ultrahigh density electrochemical sensing in the detection of H2O2, glucose, lactic acid and choline are firstly provided, the reproducibility is good, and by modifying the multiple oxidases and the receptors on the nanometer array electrode,the application of the single array in the ultrahigh density analysis of hundreds of molecules in the complex biological system can be achieved.

Description

technical field [0001] The invention belongs to the field of chemical analysis, and in particular relates to the application of nano-needle electrodes in ultra-high-density electrochemical sensing and analysis. Background technique [0002] Microarray refers to the spotting of probes on a solid support for parallel processing and detection of analytes in complex samples. Microarray has been widely used in the fields of molecular biology and genomics because of its high-throughput characteristics. The traditional microarray has about 50 points per square millimeter, with an average particle size of 100 μm. With the continuous improvement of technology, ultra-high-density arrays are emerging, which are defined as containing more than 1000 elements per square millimeter. Electrochemical analysis, as a highly sensitive, highly selective and label-free detection method, is one of the main application methods of arrays. However, realizing ultra-high-density electrochemical micro...

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Application Information

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IPC IPC(8): G01N27/327G01N27/30
CPCG01N27/305G01N27/3272G01N27/3278
Inventor 江德臣周俊宇张晶晶王伟
Owner NANJING UNIV
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