Preparation method of catfish visceral protease

A protease and fish viscera technology is applied in the field of preparation of catfish viscera protease, which can solve the problems of inconvenient operation, low protease purity, low efficiency, etc., and achieves the effects of enhancing disease prevention, improving lymphocyte activity and high purity

Inactive Publication Date: 2018-03-23
金华市川璞农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The invention realizes the value-added utilization of squid viscera, improves the added value of fishery production, reduces the pollution of squid products to the environment, and the squid protease prepared as an enzyme preparation for hydrolyzing various food proteins increases the variety of commercial proteases, but The protease extracted by this method has low purity, low efficiency and inconvenient operation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] The preparation method of catfish viscera protease comprises leaching, salting out, dialysis and purification, and the specific steps are:

[0015] 1) Extraction: After homogenizing catfish viscera with a homogenizer, add buffer to extract for 3~5 hours, and centrifuge the extract at a speed of 8000~9000r / min for 10~15min, and the obtained supernatant is the crude catfish viscera extract Disperse and grind the catfish visceral tissue into a uniform paste through a homogenizer, so that it can fully contact with the buffer, thereby fully releasing the cell contents, leaching and centrifuging, so that the solid particles and The liquid containing the cell contents is separated to obtain the catfish crude extract;

[0016] 2) Salting out: After adding saturated ammonium sulfate to the crude catfish viscera extract for precipitation, the weight-to-volume ratio of the crude catfish viscera extract to saturated ammonium sulfate is 1g:1.3~1.6ml. Centrifuge at 10000r / min for 12...

Embodiment 2

[0021] The preparation method of catfish viscera protease comprises the following steps:

[0022] 1) Extraction: After homogenizing catfish viscera with a homogenizer, add buffer to extract for 3~5 hours, and centrifuge the extract at a speed of 8000~9000r / min for 10~15min, and the obtained supernatant is the crude catfish viscera extract ;

[0023] 2) Salting out: After adding saturated ammonium sulfate to the crude catfish viscera extract for precipitation, the weight-to-volume ratio of the crude catfish viscera extract to saturated ammonium sulfate is 1g:1.6ml, centrifuge at 5°C and 10,000 r / min 13min;

[0024] 3) Dialysis: Dissolve the precipitate in phosphate buffer, dialyze in distilled water at 5°C for 30 hours, and change the distilled water every 7 hours;

[0025] 4) Purification: The dialysate was prepared by hydroxyapatite adsorption chromatography to prepare catfish visceral pure protease. The column specifications were 2 cm in diameter and 52 cm in length. The m...

Embodiment 3

[0028] The preparation method of catfish viscera protease comprises the following steps:

[0029] 1) Extraction: After the homogenizer is homogenized, add the same amount of buffer as before to extract for 4 hours, and centrifuge the extract at a speed of 9000r / min for 13 minutes, and the obtained supernatant is the crude catfish viscera extract.

[0030] 2) Salting out: After adding saturated ammonium sulfate to the crude catfish viscera extract for precipitation, the weight-to-volume ratio of the crude catfish viscera extract to saturated ammonium sulfate is 1g:1.3ml, centrifuge at 4°C and 9000 r / min 12min.

[0031] 3) Dialysis: Dissolve the precipitate in phosphate buffer, dialyze in distilled water at 6°C for 32 hours, and change the distilled water every 8 hours.

[0032] 4) Purification: The dialysate was prepared by hydroxyapatite adsorption chromatography to prepare catfish visceral pure protease. The column specifications were 5 cm in diameter and 60 cm in length. Th...

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Abstract

A preparation method of catfish visceral protease includes extraction, salting out, dialysis and purification and has the beneficial effects: extracted protease has high purity and stable activity, can be used as an enzymatic hydrolysis agent for hydrolyzing a variety of food proteins, realizes the value-added use of catfish viscera, and improves the economic added value of catfishes. At the sametime, the high-purity protease extracted by the method can improve lymphocyte activity, thereby enhancing the body ability to prevent and resist diseases, and also having unexpected effect of scavenging free radicals in organisms.

Description

technical field [0001] The invention relates to the field of aquatic product processing, in particular to the processing technology of the preparation method of catfish viscera protease. Background technique [0002] The edible fleshy part of catfish is left during processing, and the viscera are discarded. The viscera produced with mass processing not only causes ecological pollution, but also is a great waste of resources. Viscera is rich in enzymes. A large number of studies have shown that fish viscera can be used as an important source of protease. Fish viscera The content of protease is rich, and the protease contained in it is active in a wide temperature range. It is a good source of protease. The biggest advantage of using endogenous enzymes is low cost, high activity, and good combination with substrates. Therefore, after processing catfish It is of positive significance to develop and utilize the remaining viscera as raw materials for enzyme resources. Therefore,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/64
CPCC12N9/6405
Inventor 谢少康
Owner 金华市川璞农业科技有限公司
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