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A β-lactam antibiotic degrading strain pgb1 and its application

A technology of lactams and antibiotics, applied in the field of microorganisms

Inactive Publication Date: 2020-11-17
BEIJING INSTITUTE OF TECHNOLOGYGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few reports on the biodegradation of β-lactam antibiotics, and the reported degrading strains basically only have a degrading effect on a certain antibiotic in the β-lactam antibiotics

Method used

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  • A β-lactam antibiotic degrading strain pgb1 and its application
  • A β-lactam antibiotic degrading strain pgb1 and its application
  • A β-lactam antibiotic degrading strain pgb1 and its application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1. Screening of β-lactam antibiotics degrading strain PGB1

[0025] 1. Material preparation

[0026] The source of bacteria samples: fresh bacteria residues from β-lactam antibiotics manufacturers.

[0027] Beef extract-peptone medium: 5.0g beef extract, 10.0g peptone, 5.0g NaCl, distilled water, pH 7.4-7.6, sterilized at 21°C for 15min. Add 10g-15g of agar to the solid medium.

[0028] Martin's Medium: KH 2 PO 4 1.0g, MgSO 4 7H 2 O 0.5g, glucose 10.0g, peptone 5.0g, distilled water 1L, pH natural, sterilized at 115°C for 15min. Add 10g-15g of agar to the solid medium.

[0029] Inorganic salt medium: (NH 4 ) 2 NO 4 1.0g, K 2 HPO 4 0.5g, KH 2 PO 4 0.5g, MgSO 4 ·7H 2 O0.2g, NaCl0.2g, CaCl 2 0.1g, MnSO 4 h 2 O 0.01g, FeSO 4 ·7H 2 O 0.01g, distilled water 1L, pH7.0, sterilize at 121℃ for 15-20min. Add 10g-15g of agar to the solid medium.

[0030] 2. Strain screening

[0031] (1) Strain isolation and purification

[0032] Separation and purificati...

Embodiment 2

[0088] Growth and degradation characteristics of strain PGB1

[0089] 1. Strain PGB1 grows in penicillin potassium medium

[0090]The bacterial strain PGB1 was inoculated in beef extract peptone liquid medium for activation culture. According to the inoculation amount of 3-6%, the activated bacterial liquid was taken, centrifuged at 8000r / min for 5min to obtain bacterial cells, and then inoculated to 50 -1000mg / L beef extract peptone solid and liquid medium with different concentrations of penicillin potassium, culture the solid plate at 28--37°C, observe the colony size regularly; place the liquid medium at 28--37°C, Shake culture at 150-180r / min, take samples regularly, measure the OD value at 600nm, see the results image 3 .

[0091] The results showed that strain PGB1 could grow well in beef extract peptone liquid and solid medium containing 50-1000 mg / L penicillin potassium. 8-10h enters the logarithmic growth phase, and the biomass reaches the maximum at 48h.

[009...

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Abstract

The invention relates to a degrading bacterial strain PGB1 capable of degrading β-lactam antibiotics and an application thereof, belonging to the technical field of microorganisms. The strain was deposited on September 27, 2017 in the General Microbiology Center of the China Committee for the Collection of Microbial Cultures, and the preservation number is CGMCC NO.14760. The bacterial strain PGB1 of the present invention has a better degradation effect on β-lactam antibiotics, and has a degradation rate of 100.00% for penicillin potassium as the only carbon source and a concentration of 50-300 mg / L, and can be degraded and removed Inhibition of penicillin potassium against Staphylococcus aureus. The strain can be applied to degrade the relevant β-lactam antibiotics left in the production enterprise waste and the environment.

Description

technical field [0001] The invention relates to a beta-lactam antibiotic degrading strain PGB1 and its application, belonging to the technical field of microorganisms. Background technique [0002] β-lactam antibiotics are a class of antibiotics containing a β-lactam ring in their chemical structure. They have the advantages of strong bactericidal activity, low toxicity, wide indications and good clinical efficacy. They are the most widely used class of existing antibiotics. . The world consumes an average of 100,000-200,000 tons of antibiotics per year, of which β-lactam antibiotics account for 50%-70%. [0003] During the fermentation and production of β-lactam antibiotics such as penicillin and cephalosporins, a large amount of waste water containing antibiotics will be produced. If it is not properly treated and discharged into the environment, it will pollute the environment. At the same time, when antibiotics enter the body, some antibiotics cannot be completely abso...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A62D3/02C12R1/05A62D101/28
CPCA62D3/02A62D2101/28C12N1/205C12R2001/05
Inventor 李艳菊杨文娟杨正礼闫峰
Owner BEIJING INSTITUTE OF TECHNOLOGYGY