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Synchronous extracting and fermenting method for preparing natural astaxanthin and other carotenoids

A technology of natural astaxanthin and carotene, which is applied in the field of simultaneous extraction and fermentation for the preparation of natural astaxanthin and other carotenoids, can solve the problems of high production cost, increased separation cost, and difficulty in extraction, so as to increase production and production Efficiency, simplification of the subsequent purification process, and the effect of reducing production costs

Inactive Publication Date: 2018-03-30
湖州爱蔻思生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above-mentioned preparation methods all have their own problems, and chemical synthesis has safety problems and is not welcomed by consumers.
The preparation cost of extraction from natural substances such as shrimp and crab is high, and the productivity of algae or yeast is low and the extraction is more difficult due to the strong cell wall, and the production of algae is mainly astaxanthin ester instead of pure astaxanthin, further increasing the separation cost

Method used

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  • Synchronous extracting and fermenting method for preparing natural astaxanthin and other carotenoids
  • Synchronous extracting and fermenting method for preparing natural astaxanthin and other carotenoids
  • Synchronous extracting and fermenting method for preparing natural astaxanthin and other carotenoids

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Step 1) prepare 5L culture medium, comprise 10g / L glucose, 2g / L (NH 4 ) 2 SO 4 , 4.2g / L KH 2 PO 4 and 11.24g / L K 2 HPO 4 , 1.7g / L citric acid, 0.5g / L MgSO 4 And 10mL / L trace element solution. Among them, the trace element solution contains 0.25g / L CoCl 2 ·6H 2 O, 1.5g / L MnSO 4 4H 2 O, 0.15g / L CuSO 4 2H 2 O, 0.3g / L H 3 BO 3 , 0.25g / LNa 2 MoO4· 2 h 2 O, 0.8g / L Zn(CH 3 COO) 2 , 5g / L iron (III) citrate and 0.84g / L EDTA, the pH of the trace element solution is 8.0.

[0027] Step 2) Sterilize the culture medium at 121° C. for 15-20 minutes. (Note, the glucose solution should be sterilized separately before mixing with other ingredients).

[0028] Step 3) Next, take 100mL of culture medium and put them into identical #1 and #2, two 250mL fermentors, and place the fermentors at a temperature of 121°C.

[0029] Step 4) Inoculate the Escherichia coli strain E with high astaxanthin production through genetic engineering into 20mL medium (the medium is taken f...

Embodiment 2

[0035] Step 1) prepare 5L culture medium, comprise 10g / L glucose, 2g / L (NH 4 ) 2 SO 4 , 4.2g / L KH 2 PO 4 and 11.24g / L K 2 HPO 4 , 1.7g / L citric acid, 0.5g / L MgSO 4 And 10mL / L trace element solution. Among them, the trace element solution contains 0.25g / L CoCl 2 ·6H 2 O, 1.5g / L MnSO 4 4H 2 O, 0.15g / L CuSO 4 2H 2 O, 0.3g / L H 3 BO 3 , 0.25g / LNa 2 MoO4· 2 h 2 O, 0.8g / L Zn(CH 3 COO) 2 , 5g / L iron (III) citrate and 0.84g / L EDTA, the pH of the trace element solution is 8.0.

[0036] Step 2) Sterilize the culture medium at 121° C. for 15-20 minutes. (Note, the glucose solution should be sterilized separately before mixing with other ingredients).

[0037] Step 3) Next, take 100mL of medium and put them into identical #3 fermenter, #4 fermenter, #5 fermenter, and #6 fermenter, all of which are 250mL fermenters, and put the fermentors into Placed at a temperature of 121°C.

[0038] Step 4) Inoculate the Escherichia coli strain E with high astaxanthin production th...

Embodiment 3

[0044] Step 1) prepare 5L culture medium, comprise 10g / L glucose, 2g / L (NH 4 ) 2 SO 4 , 4.2g / L KH 2 PO 4 and 11.24g / L K 2 HPO 4 , 1.7g / L citric acid, 0.5g / L MgSO 4 And 10mL / L trace element solution. Among them, the trace element solution contains 0.25g / L CoCl 2 ·6H 2 O, 1.5g / L MnSO 4 4H 2 O, 0.15g / L CuSO 4 2H 2 O, 0.3g / L H 3 BO 3 , 0.25g / LNa 2 MoO4· 2 h 2 O, 0.8g / L Zn(CH 3 COO) 2 , 5g / L iron (III) citrate and 0.84g / L EDTA, the pH of the trace element solution is 8.0.

[0045] Step 2) Sterilize the culture medium at 121° C. for 15-20 minutes. (Note, the glucose solution should be sterilized separately before mixing with other ingredients).

[0046] Step 3) Next, take 100mL of the culture medium and put them into the same #7 fermenter, #8 fermenter, #9 fermenter, #10 fermenter, #11 fermenter, #12 fermenter, #13 fermenter tank, #14 fermenter, all fermenters were 250 mL, and the fermentors were placed at a temperature of 121°C.

[0047] Step 4) Inoculate the...

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Abstract

The invention relates to a synchronous extracting and fermenting method for preparing natural astaxanthin and other carotenoids. The synchronous extracting and fermenting method is characterized in that in the fermenting process, when the cell concentration OD reaches 0.6-140, an extracting agent is added, the volume ratio of the extracting agent to fermenting liquid is 1%-100%, the culture temperature is 15-40 DEG C, and the pH value is 5-10. Compared with the prior art, the synchronous extracting and fermenting method has the following advantages that by adding the extracting agent in the fermenting process, not only are the yield and productivity of astaxanthin increased, but also a subsequent purification technology is simplified, and therefore the production cost is effectively lowered.

Description

technical field [0001] The invention relates to a synchronous extraction and fermentation method, in particular to a synchronous extraction and fermentation method for preparing natural astaxanthin and other carotenoids. Background technique [0002] Carotenoids are natural pigments that can be used as feed additives, food additives, pharmaceuticals, and cosmetics. Carotenoids include, for example, astaxanthin, canthaxanthin, zeaxanthin, β-cryptoxanthin, lycopene, β-carotene ( β-carotene), Lucilia xanthin (phoenicoxanthin), calendula xanthin (adonixanthin), echinenone (echinenone) and 3-hydroxyechinenone (3-hydroxyechinenone). Among carotenoids, astaxanthin has higher antioxidant properties and more extensive biological activities, and can be used as a body color improving agent for farmed fish such as salmon, trout, sea bream, etc. or as an egg yolk improving agent for poultry and other feed additives. In addition, astaxanthin, as a safe natural food additive or healthy ...

Claims

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Application Information

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IPC IPC(8): C12P23/00C07C403/24
CPCC07C403/24C12P23/00
Inventor 张聪强
Owner 湖州爱蔻思生物科技有限公司
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