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Method for producing astaxanthin from chromochloris zofingiensis

An astaxanthin and green algae technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of easy contamination, hindering astaxanthin, and long cultivation time, so as to shorten the cultivation time and improve the Production efficiency, the effect of improving yield and productivity

Pending Publication Date: 2019-09-27
SOUTH CHINA UNIV OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the content of astaxanthin produced by Haematococcus pluvialis is the highest, and it has been used in the industrial production of astaxanthin, but its cell density is low, the culture time is long and it is easy to pollute. In addition, the induction and accumulation of astaxanthin Requires extremely high light intensity, these problems hinder the further commercial application of astaxanthin

Method used

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  • Method for producing astaxanthin from chromochloris zofingiensis
  • Method for producing astaxanthin from chromochloris zofingiensis
  • Method for producing astaxanthin from chromochloris zofingiensis

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Experimental program
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Effect test

Embodiment 1

[0042] 1. Effects of mixed carbon sources on the accumulation of astaxanthin in Chlorella zoffensis

[0043] 1.1 Algae activation and rapid preparation of seed solution

[0044] Pick a single algae colony from the medium plate, inoculate it into a 250mL Erlenmeyer flask of Bristol’s medium containing 10g / L glucose (the filling volume is 100mL), and inoculate it under the light of 10μmol.m -2 .s -1 , in a constant temperature incubator with a temperature of 26° C. and a rotational speed of 150 r / min for continuous cultivation for 4-5 days.

[0045] The above-mentioned activated seed solution was inserted into Bristol medium (seeding density was 2.30g / L), wherein the glucose concentration in the medium was 30g / L, sodium nitrate was the carbon source, and the carbon-nitrogen ratio was 34. In the light of 10μmol.m -2 .s -1 , and cultivated in a constant temperature incubator with a temperature of 26°C and a rotation speed of 150r / min.

[0046] 1.2 Effects of mixed carbon sour...

Embodiment 2

[0055] 2. The effect of inoculation density on the accumulation of astaxanthin in Chlorella zoffii

[0056] 2.1 Algae activation and rapid preparation of seed solution

[0057] The activation of Chlorella zoff and the rapid preparation of the seed solution were as described in 1.1.

[0058] 2.2 The effect of inoculation density on the accumulation of astaxanthin in Chlorella zoffii

[0059] Nitrogen-free Britol medium was used as the basic medium, the initial glucose concentration was 20g / L, and the inoculation density was set to: 1.22×10 8 , 0.61×10 8 , 0.32×10 8 and 0.24×10 8 cfu / mL. Other culture conditions are the same as those described in 1.2.

[0060] After the cultivation, the biomass concentration of the green algae cells was measured; centrifuged to collect, the supernatant was removed, the algae mud was collected and freeze-dried, and the pigment composition in the algae cells was analyzed.

[0061] 2.3 Experimental results

[0062] from Figure 2a-2b And T...

Embodiment 3

[0068] 3. The effect of light intensity on the accumulation of astaxanthin in Chlorella zoffii

[0069] 3.1 Algal seed activation and rapid preparation of seed solution

[0070] The activation of Chlorella zoff and the rapid preparation of the seed solution were as described in 1.1.

[0071] 3.2 The effect of light intensity on the accumulation of astaxanthin in Chlorella zoffii

[0072] Nitrogen-free Britol medium was used as the basic medium, the initial glucose was 20g / L, and the light gradient was set to: 88±8, 160±5, 240±6 and 320±5μmol.m -2 .s -1 Other culture conditions are the same as those described in 1.2.

[0073] After the cultivation, the biomass concentration of the green algae cells was measured; centrifuged to collect, the supernatant was removed, the algae mud was collected and freeze-dried, and the pigment composition in the algae cells was analyzed.

[0074] 3.3 Experimental results

[0075] from Figure 3a-3b And Table 3 shows that when the light inte...

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Abstract

The invention discloses a method for producing astaxanthin from chromochloris zofingiensis. The method comprises steps as follows: S1, the chromochloris zofingiensis is subjected to rapid plant expanding, cell density is increased, and the chromochloris zofingiensis is inoculated to a shake flask culture medium for stress induction; sodium acetate concentration of mixed carbon source concentration is 0-3.0 g / L, inoculation density is 0.24*10<8>-1.22*10<8> cfu / mL, illumination intensity is 80-320 mu mol s<-1>.m<-2>, and concentration of a hydrogen peroxide inducer is 0-137.5 mg / L; S2, experiment conditions obtained by the shake flask in S1 are subjected to enlargement verification in the light fermentation tank in different operating modes. According to the method for producing astaxanthin from the chromochloris zofingiensis, yield and productive rate of astaxanthin produced from the chromochloris zofingiensis can be increased, culture time and cost are greatly reduced, production efficiency is improved, and the method has great significance in production of astaxanthin from the chromochloris zofingiensis.

Description

technical field [0001] The invention relates to a method for efficiently producing astaxanthin, which belongs to the technical field of bioengineering fermentation, and in particular relates to a method for producing astaxanthin by using Zoff color green algae. Background technique [0002] Astaxanthin is a fat-soluble purple-red carotenoid that is ubiquitous in a variety of microorganisms and marine organisms. The color of some crustaceans such as shrimps, crabs and salmonids is due to the presence of Caused by astaxanthin. Astaxanthin has strong coloring function, anti-oxidation, anti-cancer and other physiological activities, and has a wide range of applications in feed, food, nutrition and pharmaceutical industries. [0003] The sources of astaxanthin are divided into natural extraction and artificial synthesis. Naturally extracted astaxanthin is mainly derived from algae, yeast, some fish and shrimp, etc. Compared with synthetic astaxanthin, natural astaxanthin has be...

Claims

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Application Information

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IPC IPC(8): C12P23/00C12R1/89
CPCC12P23/00
Inventor 魏东姜雪亚陈俊辉
Owner SOUTH CHINA UNIV OF TECH
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