Colloidal gold immunochromatography test strip for Brazil nut allergen in food and preparation method of colloidal gold immunochromatography test strip

A technology of immunochromatographic test paper and colloidal gold, which is applied in the direction of analysis materials, measuring devices, instruments, etc., can solve the problems that have not been established, affect the effective implementation of science, and have not released Brazil nut allergen components, etc., to achieve strong specificity and sensitivity high effect

Inactive Publication Date: 2018-03-30
ANHUI UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, no standard detection method for Brazil nut allergen components has been released at home and abroad. The detection of Brazil nuts abroad usually uses a commercial allergen protein immunochromatographic rapid detection technology. Domestic

Method used

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  • Colloidal gold immunochromatography test strip for Brazil nut allergen in food and preparation method of colloidal gold immunochromatography test strip
  • Colloidal gold immunochromatography test strip for Brazil nut allergen in food and preparation method of colloidal gold immunochromatography test strip
  • Colloidal gold immunochromatography test strip for Brazil nut allergen in food and preparation method of colloidal gold immunochromatography test strip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Preparation of Colloidal Gold Immunochromatographic Test Strips for Brazil Nut Allergens in Food

[0025] The preparation of the colloidal gold immunochromatographic test strip of Brazil nut allergen in food comprises the following steps:

[0026] (1) Solution preparation:

[0027] Preparation of trisodium citrate: Accurately weigh 0.1g of trisodium citrate, dissolve it in ultrapure water, and dilute to 10mL, filter through a 0.22μm filter membrane, and prepare and use immediately;

[0028] Preparation of chloroauric acid solution: Accurately weigh 1.0g of trisodium citrate, dissolve it in ultrapure water, and dilute to 100mL, filter with a 0.22μm filter membrane, and store the brown reagent bottle at 4°C for later use;

[0029] Preparation of potassium carbonate solution: Accurately weigh 1.38g of potassium carbonate, dissolve it in ultrapure water, and dilute to 50mL, filter through a 0.22μm filter membrane, and store at 4°C for later use;

[0030] Prepara...

Embodiment 2

[0043] Example 2 Sensitivity determination of colloidal gold immunochromatographic test strips for Brazil nut allergens in food

[0044] Use the sample diluent to dilute the standard to different concentrations: 100000ppm, 10000ppm, 1000ppm, 100ppm, 10ppm, 5ppm, 2ppm, 1ppm, 0ppm, use the test paper prepared in Example 1 for detection, and use the sample diluent as a negative control (0ppm) , observe the results after 5 minutes, the test results are as follows figure 1 shown. The results show that 100,000ppm is positive at a high concentration, and the C line is colored; 10,000ppm is positive at a higher concentration, and the TC line is colored; 2-1000ppm is positive, and the THC line is colored; 0ppm is a negative control, and the HC line is colored. The test strip has a sensitivity of 1 ppm.

Embodiment 3

[0045] Example 3 Specificity Determination of Colloidal Gold Immunochromatographic Test Strips for Brazil Nut Allergens in Food

[0046] Take pecan, gluten (Gluten), almond (Almond), hazelnut (Hazelunt), egg (Egg), peanut (Peaunt) respectively as test samples, grind and dilute to 100ppm after crushing, and use Example 1 to prepare The test strips are tested, and the results are observed after 5 minutes. The test results are as follows: figure 2 shown. The results showed that Pecan, Gluten, Almond, Hazelunt, Egg, and Peanut all showed C lines at 100ppm, and the test paper and Pecan ( Pecan), gluten (Gluten), almond (Almond), hazelnut (Hazelunt), egg (Egg), peanut (Peanut) have no cross-reaction, showing that the test strip has good specificity.

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Abstract

The invention discloses a colloidal gold immunochromatography test strip for Brazil nut allergen in food and a preparation method of the colloidal gold immunochromatography test strip. The colloidal gold immunochromatography test strip comprises a water absorbing pad, a nitrocellulose membrane, a gold mark pad and a sample pad which are sequentially connected, and further comprises a bearing baseplate, wherein a detection line, an antigen line and a quality control line are arranged on the nitrocellulose membrane; an anti-Brazil-nut-protein monoclonal antibody-colloidal gold marker is fixed onthe gold mark pad; the detection line of the nitrocellulose membrane is coated with another Brazil nut protein capturing monoclonal antibody; and the antigen line coats Brazil nut protein; and the quality control line coats goat anti mouse IgG. When one quality control line and one antigen line appear on the test strip, the symptom is negative, and when the detection line appears (no matter whether the antigen line appears or not), the symptom is positive. Compared with a conventional Brazil nut protein allergen detection technology, the colloidal gold immunochromatography test strip is higher in sensitivity and strong in specificity, can visually evaluate the concentration of the allergen and can be directly used for food detection.

Description

technical field [0001] The invention belongs to the technical field of food safety detection, and in particular relates to a colloidal gold immunochromatographic test strip for detecting Brazil nut allergens in food and a preparation method thereof. Background technique [0002] Nutritious, Brazil nuts are common and widely consumed tree nuts. Allergic symptoms caused by Brazil nuts include itchy throat, swollen lips, dysphonia, dyspnea, asthma, macular rash, etc. Therefore, Brazil nuts are an important category of food allergen label management. So far, no standard detection method for Brazil nut allergen components has been released at home and abroad. The detection of Brazil nuts abroad usually uses a commercial allergen protein immunochromatographic rapid detection technology. Domestically, Brazil nuts have not yet established any industrial application level. general method. This situation has seriously affected the scientific and effective implementation of relevant ...

Claims

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Application Information

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IPC IPC(8): G01N33/558G01N33/577
CPCG01N33/558G01N33/577
Inventor 唐恒立周祚琴赵亮杰森 M.罗博特姆肯尼斯 H.鲁亨利格赖斯张部昌唐小迪王敏娜
Owner ANHUI UNIVERSITY
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