Multi-layered oriented nano-fiber artificial dura mater and preparation method thereof

Abstract

The invention provides a multi-layered oriented nano-fiber artificial dura mater. The multi-layered oriented nano-fiber artificial dura mater comprises three layers, namely an inner hydrophobic layer,an outer hydrophilic layer and an intermediate woven layer, wherein by virtue of the 'similarity compatibility principle', the inner layer and the outer layer are reliably connected together throughthe intermediate woven layer; and the outer layer adopts a layered structure, a second layer of the outer layer is of a groove structure consisting of oriented nano-fiber thin strips, and the length directions of grooves are consistent with the directions of nano-fibers. The invention also provides a preparation method of the multi-layered oriented nano-fiber artificial dura mater. The preparationmethod comprises the following steps: the inner hydrophobic layer is prepared by electrospinning; the woven layer is prepared; and the outer layer is prepared on the woven layer in an electrospinningmanner, and finally, the artificial dura mater is obtained. According to the artificial dura mater, the groove structure of the outer layer plays fixing and guiding roles on newborn cranial dura tissues; the problem of layering of the artificial dura mater is effectively solved by the intermediate woven layer; and the multi-layered oriented nano-fiber artificial dura mater can be cut at random and has a wide application prospect.

Description

technical field [0001] The invention relates to a multilayer oriented nanofiber artificial dura mater and a preparation method thereof, belonging to the technical field of electrospinning. Background technique [0002] Dural defects are common in neurosurgery clinical work. Open craniocerebral injuries (industry, traffic, war, etc.), tumor erosion, congenital meningeal defects and other craniocerebral diseases can cause dural defects. The dural defect needs to be repaired in time to prevent cerebrospinal fluid from overflowing, to prevent the swelling of the brain and the compression of atmospheric pressure, otherwise it will endanger human life. Dural defects can also cause complications such as intracranial infection, brain adhesions, and subcutaneous fluid, often causing diseases such as headaches and brain dysfunction. [0003] The dura mater is the outermost layer of the membrane structure wrapped around the brain and spinal cord. It is a relatively strong connective t...

AI Technical Summary

Problems solved by technology

Although this artificial dura mater can realize the purpose of dura mater regeneration and prevention of cicatrix and adhesion, there are following disadvantages: the first, the inner layer is an orientation copolymer fiber membrane, which can accelerate the growth rate of meningeal cells, but the artificial dura mater and The patient's own dura mater is not strong enough, and there are hidden dangers such as artificial meningeal dislocation and folds in the meningeal repair area

Second, during use, the damaged dura mater is covered with the inner layer first, while the outer layer of the human dura mater is mainly composed of collagen and a small amount of fibroblasts, and the advantages of cell growth induced by oriented fibers have not been fully utilized

Third, the preparation efficiency of oriented fiber membrane is low, resulting in high cost of artificial meninges

Although the artificial dura mater can achieve the purpose of dura mater regeneration, it has the following disadvantages: the inner layer is prepared by electrospinning process, the nanofibers in the inner layer are disorderly, and the dura mater recovery period is too long

Images