Preparation and testing method of a core-shell enhanced Raman spectroscopy substrate
A Raman spectroscopy substrate, Raman spectroscopy technology, applied in Raman scattering, measurement devices, material analysis by optical means, etc., can solve problems such as single functionality, and achieve the effect of a simple method
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Embodiment 1
[0030] Add 440mg of anhydrous sodium citrate to 2.5×10 -4 M HAuCl 4 ·3H 2 In 100mL boiling aqueous solution of O, stir vigorously for 15 minutes and cool down to obtain a water dispersion solution of gold nanoparticles, with a concentration of about 50 μg mL -1 ; 0.4mL 50μg mL -1 Aqueous dispersion of gold nanoparticles with 2mL of 2mg mL -1 (PVP) 2 -(PNIPAM-b-PAA) 2The aqueous solution and 1.6mL deionized water were mixed evenly, and after standing for two days, centrifuged three times, and the precipitate was redispersed in 4mL aqueous solution to obtain (PVP) 2 -(PNIPAM-b-PAA) 2 loaded gold nanoparticles.
[0031] figure 1 and figure 2 Is the Au@(PVP) 2 -(PNIPAM-b-PAA) 2 Schematic diagram of the structure of nanoparticles and TEM images under different conditions. From figure 1 and figure 2 It can be seen that changing the temperature and pH can well change the thickness and aggregation state of the shell.
[0032] Configure 5μg mL -1 Au@(PVP) 2 -(PNIPAM-...
Embodiment 2
[0035] Configure 5μg mL -1 Au@(PVP) 2 -(PNIPAM-b-PAA) 2 with 10 -6 The mixed solution of M R6G was put into a capillary tube after standing for 24 hours for Raman spectroscopy test. The test conditions of Raman spectroscopy are: excitation wavelength of 633nm, laser energy of 17mW, and exposure time of 10s. The temperature of the solution is controlled by a hot stage, and the pH is controlled by adding NaOH or HCl solution to the mixture. The temperature control range is 25-55°C, and the pH control range is 5-13.
[0036] This example examines Au@(PVP) under different temperature and pH conditions 2 -(PNIPAM-b-PAA) 2 Raman spectrum signal enhancement effect on R6G molecules. Via R6G 1359cm -1 The enhancement factor calculated from the peak intensity at the peak is plotted against the temperature and pH, and the Figure 4 . From Figure 4 It can be seen from the figure that Au@(PVP) 2 -(PNIPAM-b-PAA) 2 The enhancement of the Raman signal of R6G molecules shows a gr...
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