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Rotavirus detection reagent card, kit, and application thereof

A detection kit, rotavirus technology, applied in measurement devices, instruments, scientific instruments, etc., can solve the problems of disease diagnosis obstacles, low accuracy of quantitative detection results, etc., to improve accuracy and precision, reduce errors, avoid the effect of mutual interference

Inactive Publication Date: 2018-04-06
SHANGHAI CHEMTRON BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Judging by the color of a test line is easily affected by the brightness and color of the environment, and is also affected by the subjective judgment of the user, resulting in a large error, which makes the detection results, especially the quantitative detection results, less accurate, which is the cause of disease. Diagnosis brings obstacles

Method used

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  • Rotavirus detection reagent card, kit, and application thereof

Examples

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Comparison scheme
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preparation example Construction

[0045] The embodiment of the present invention also provides a preparation method of the above-mentioned rotavirus detection reagent card, comprising the following steps: 1) spraying latex particles-the first anti-rotavirus antibody and latex on the glass cellulose membrane Particle-test antigen mixed solution; 2) use the first pretreatment solution to carry out the first pretreatment on the nitrocellulose membrane corresponding to the T1 line to be sprayed, and spray the nitrocellulose membrane after the first pretreatment The second anti-rotavirus antibody solution; 3) use the second pretreatment liquid to carry out the second pretreatment to the nitrocellulose membrane corresponding to the T2 line to be sprayed, and spray the nitrocellulose membrane after the second pretreatment Spot the rotavirus antigen solution; 4) Spray the antibody solution of the test antigen on the nitrocellulose membrane at the preset position.

[0046] In one example, the first pretreatment liquid ...

Embodiment 1

[0054] 11. Preparation of rotavirus detection reagent card, the specific method includes the following steps:

[0055] 111. Preparation of latex particle-first anti-rotavirus antibody and latex particle-test antigen mixed solution:

[0056] Add the required amount of the above-mentioned rotavirus VP6 monoclonal antibody and the volume of carboxylated red polystyrene latex particles to the preset volume of 0.05M PBS, and after ultrasonication, centrifugation and other operations, the latex particles obtained After the primary anti-rotavirus antibody solution is diluted, the absorbance value of the suspension at 500nm is measured with a spectrophotometer to obtain the latex particle-first anti-rotavirus antibody solution. Add the required amount of rabbit IgG and the volume of carboxylated red polystyrene latex particles to the preset volume of 0.05M PBS. After ultrasonication, centrifugation and other operations, the obtained latex particles-test antigen solution is diluted. ,...

Embodiment 2

[0078] 21. Preparation of rotavirus detection reagent card.

[0079] The specific preparation method refers to the description of steps 111-1111 in Example 1, wherein, the difference is that the first pretreatment liquid formula in this embodiment is: arginine 1.75g / L, potassium hydrogen phthalate 1.5g / L, sodium hydroxide 0.3g / L, citric acid 2.5g / L, solvent is water, pH value=6.0. The formula of the second pretreatment liquid is: asparagine 2.0g / L, disodium hydrogen phosphate 0.7g / L, rhamnose 3.5g / L, glycine 3.0g / L, solvent is water, pH=8.0.

[0080] 22. Use the rotavirus detection reagent card provided in this example to detect the rotavirus antigen.

[0081] For the detection process, refer to step 121 and step 122 in Embodiment 1. The test results were 31IFU / ml, 292IFU / ml, 1210IFU / ml, 6080IFU / ml respectively, and no rotavirus antigen was detected in the blank control.

[0082] The above results show that the accuracy of antigen detection by the rotavirus antigen provided...

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Abstract

The invention relates to the field of latex-process immunochromatography, and particularly relates to a rotavirus detection reagent card including, in a successively arranged manner, a sample loadingpad, a glass cellulose membrane, a nitro-cellulose membrane and a water absorption pad, wherein the glass cellulose membrane contains latex particles-first anti-rotavirus antibody and latex particles-detection antigen; the nitro-cellulose membrane is successively provided with a first detection line, a second detection line and a control line, the first detection line coating a second anti-rotavirus antibody, the second detection line coating a rotavirus antigen, and the control line coating an antibody of the detection antigen.

Description

technical field [0001] The invention relates to the field of latex immunochromatography, in particular to a rotavirus detection reagent card, a kit and applications thereof. Background technique [0002] All immunodiagnostic reagents, whether qualitative or quantitative, are based on the principle of immune response, that is, antigen-antibody complexes are produced by specific combination of antigen and antibody under certain conditions, and the antigen or antibody is labeled with a tracer. Antibodies, to finally realize the detection and analysis of the reaction products. [0003] In the prior art, a test line is set on the immunodiagnostic reagent card, and it is determined whether the sample contains pathogenic antigens according to the color of the test line. Judging by the color of a test line is easily affected by the brightness and color of the environment, and is also affected by the subjective judgment of the user, resulting in a large error, which makes the detect...

Claims

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Application Information

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IPC IPC(8): G01N33/577
CPCG01N33/577
Inventor 丁晓辉
Owner SHANGHAI CHEMTRON BIOTECH