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An infectious bronchitis virus low virulent strain, a constructing method thereof and applications of the strain

A technology for chicken infectiousness and bronchitis, which is applied in the directions of viruses, antiviral agents, and viral antigen components, can solve the problems of less use, and achieve the effect of good immunogenicity and stable heredity

Active Publication Date: 2018-04-10
诗华动保科技(北京)有限公司
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  • Abstract
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AI Technical Summary

Problems solved by technology

The LTD3 strain was weakened by Liu Shengwang, a researcher at the Harbin Veterinary Research Institute of the Chinese Academy of Agricultural Sciences, using a strain that is prevalent in China and is currently less used in the market

Method used

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  • An infectious bronchitis virus low virulent strain, a constructing method thereof and applications of the strain
  • An infectious bronchitis virus low virulent strain, a constructing method thereof and applications of the strain
  • An infectious bronchitis virus low virulent strain, a constructing method thereof and applications of the strain

Examples

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Effect test

Embodiment 1

[0023] Example 1 Construction and identification of avian infectious bronchitis virus attenuated strain HC13-91 strain

[0024] (1) Virus identification

[0025] Using the currently popular QX-Like genotype chicken IBV HC13 strain as the attenuated female parent, the HC13 strain was purified using the limiting dilution method, and the chicken embryo allantoic fluid with curling and reduced body weight at the highest dilution was used as the next inoculation Virus seeds are purified three times in sequence (P1~P3). After the three purifications were completed, the purified allantoic fluid was inoculated with 10-day-old SPF chicken embryos for passage (P4), and the allantoic fluid was identified by molecular biology determination of its S1 gene and serological methods.

[0026] The results of genetic evolution analysis of serology and molecular biology showed that the HC13 strain and the attenuated HC13-91 strain are both QX-Like genotype strains, which match the current circulating s...

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Abstract

An infectious bronchitis virus low virulent strain, a constructing method thereof and applications of the strain are disclosed. An infectious bronchitis virus high virulent strain of the QX-Like genotype, which is separated in the field, is adopted as a female parent, and is subjected to 100 or above generations of continuous passage through chicken embryos to obtain the attenuated virulent strain. The microbial preservation accession number of the low virulent strain is CGMCC No.13292. The S1 sequence of genome of the low virulent strain is shown as SEQ ID No.1. Chickens immunized with the low virulent strain do not die, and are free of obvious tissue organ lesions and clinical symptoms, thus proving that the low virulent strain is safe to chickens and good in safety. The challenge protection rate of the low virulent strain for immunized chickens is 80-100% so that the low virulent strain has good immunogenicity. Virulence enhancement tests prove that virulence of the low virulent strain is not enhanced, and the low virulent strain is stable in heredity and is suitable as a vaccine strain or a virus seed for preventing and treating infectious bronchitis.

Description

Technical field [0001] The present invention relates to an attenuated strain of Infectious Bronchitis Virus (Infectious Bronchitis Virus), and further relates to a live vaccine prepared by the attenuated strain of Infectious Bronchitis Virus and its application in the prevention and treatment of infectious bronchitis in chickens. The field of prevention and treatment of sexual bronchitis. Background technique [0002] Infectious Bronchitis (IB) is an acute, highly contagious respiratory disease in chickens caused by viruses. It mainly affects the respiratory system, genitourinary system and digestive system. It is characterized by coughing, sneezing, tracheal rales, runny nose in young chickens, decline in the number and quality of laying hens, and can directly lead to the death of chicks, kidney disease and fallopian tubes. Permanent degradation. The disease has a worldwide distribution and is extremely harmful to the chicken industry. [0003] Because IBV has many serology and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/00A61K39/215A61P31/14C12R1/93
CPCA61K39/12A61K2039/5254C12N7/00C12N2770/20021C12N2770/20034
Inventor 李海燕张坦杨国良张立艳
Owner 诗华动保科技(北京)有限公司
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