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Screening method of feed probiotics capable of preventing piglet epidemic diarrhea

A technology for porcine epidemic diarrhea and epidemic diarrhea, which is applied in the direction of biochemical equipment and methods, and the determination/inspection of microorganisms. The effect of adapting, saving manpower and avoiding blindness

Inactive Publication Date: 2018-04-10
WUHAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although a large number of probiotics have been screened in my country and widely used in production practice, these probiotics are mainly used to promote the digestion and absorption of nutrients in the intestinal tract of animals and promote growth, and many probiotics do not come from the body. Animals, after entering the body of the host, it is difficult to adapt to the host, and it is difficult to exert its exact effect
Moreover, the existing probiotic screening technologies mainly include in vitro antibacterial methods and in vivo bacterial attack methods, which have the disadvantages of blindness, low efficiency, poor pertinence, and mostly exogenous bacteria.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] (1) Select 5 pig farms where PED broke out, observe the typical clinical symptoms of piglets with watery diarrhea and vomiting, and conduct necropsy of the pathological damage of piglets with yellow liquid in the intestines, mesenteric congestion, mesenteric lymph node edema, and shortened small intestinal villi. Combined with the qualitative PCR detection of the PED pathogen, 25 disease-resistant piglets with typical clinical symptoms and obvious pathological damage and 25 disease-resistant piglets without typical clinical symptoms and obvious pathological damage were selected from the group of piglets that were positive in the qualitative PCR test; Collect intestinal chyme samples from the intestines of diseased piglets and disease-resistant piglets, and store them at -80°C for later use.

[0077] (2) Extract the genomes of the microbial flora in the intestinal chyme samples of diseased piglets and disease-resistant piglets with a kit specially produced by Qiagen Compa...

Embodiment 2

[0083] (1) Forty 3-day-old PEDV-negative piglets purchased from pig farms that had not been immunized against PEDV were fed with artificial milk replacer as experimental piglets, and were randomly divided into groups I, II, III and IV. group, 10 piglets in each group, wherein, group Ⅰ is the control group, group Ⅱ, group Ⅲ and group Ⅳ are probiotic groups;

[0084] (2) After the probiotics (including Lactobacillus johnsonii and Enterococcus faecium) screened in Example 1 were cultured in vitro, the test piglets were inoculated with probiotics from the morning of the 3rd day of age in a 10-fold increasing manner. , until the test piglets grow to 7 days old, the inoculation dose on the first day is 5×10 2 cfu / 100uL, and then the subsequent daily inoculation doses were 5×10 3 cfu / 100uL, 5×10 4 cfu / 100uL, 5×10 5 cfu / 100uL, 5×10 6 cfu / 100uL; Among them, piglets in group I were inoculated with 100uL of normal saline, piglets in group II were inoculated with Lactobacillus johnson...

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Abstract

The invention discloses a screening method for feeding probiotics against piglet epidemic diarrhea, which comprises the following steps: screening piglets infected with PEDV and disease-resistant piglets and collecting intestinal content samples; piglets infected with PEDV and resistant piglets High-throughput sequencing of intestinal microbial flora of piglets; comparative analysis of intestinal microbial metagenomics between piglets with PEDV infection and disease-resistant piglets; and screening and identification of probiotics against PEDV infection. The screening method for feeding probiotics for anti-epidemic diarrhea of ​​piglets proposed by the present invention specifically screens out probiotics with anti-epidemic diarrhea function from the huge microbial flora of disease-resistant piglets, which is highly targeted and avoids It eliminates the blindness of screening, improves screening efficiency, and saves a lot of manpower, material resources and time.

Description

technical field [0001] The invention relates to the technical field of feed additives for piglets, in particular to a screening method for feeding probiotics against piglet epidemic diarrhea. Background technique [0002] Probiotics are playing an increasingly important role in safeguarding human and animal health or preventing certain diseases, especially under the premise that antibiotics will be completely banned in my country, intestinal health and diarrhea will be bottlenecks that threaten the development of animal husbandry in my country. Among them, porcine epidemic diarrhea caused by porcine epidemic diarrhea virus (Porcine Epidemic Diarrhea Virus, PEDV) is a kind of acute intestinal infectious disease of pigs, and its main clinical symptoms are that feed intake is greatly reduced, watery diarrhea and Accompanied by vomiting, dehydration in severe cases, all ages and breeds can be infected, and it is currently one of the main diseases that cause early death of piglet...

Claims

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Application Information

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IPC IPC(8): C12Q1/6869C12Q1/04
CPCC12Q1/6869C12Q2535/122C12Q2537/165
Inventor 吴涛侯永清丁斌鹰易丹赵迪
Owner WUHAN POLYTECHNIC UNIVERSITY