Screening method of feed probiotics capable of preventing piglet epidemic diarrhea

A technology for porcine epidemic diarrhea and epidemic diarrhea, which is applied in the direction of biochemical equipment and methods, and the determination/inspection of microorganisms. The effect of adapting, saving manpower and avoiding blindness

Inactive Publication Date: 2018-04-10
WUHAN POLYTECHNIC UNIVERSITY
View PDF2 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although a large number of probiotics have been screened in my country and widely used in production practice, these probiotics are mainly used to promote the digestion and absorption of nutrients in the intestinal tract of animals and promote growth, and many probiotics do not come from the body. Animals, after entering the body o

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0075] Example 1

[0076] (1) Select 5 pig farms with PED outbreaks and observe the typical clinical symptoms of piglet watery diarrhea and vomiting and autopsy for pathological damage caused by piglets’ intestines filled with yellow fluid, mesenteric congestion, mesenteric lymph node edema, and small intestinal villi shortening. Combined with the qualitative PCR detection of the PED pathogen, 25 piglets each with disease-resistant piglets with typical clinical symptoms and obvious pathological damage and 25 disease-resistant piglets without typical clinical symptoms and obvious pathological damage were selected from the piglet group that was positive by qualitative PCR; Collect chyme samples from the intestines of diseased piglets and disease-resistant piglets, and store them at -80°C for later use.

[0077] (2) Use Qiagen's special kit for extracting microbial genomes in chyme and feces to extract the genome of the microbial flora in the intestinal chyme samples of diseased pigle...

Example Embodiment

[0082] Example 2

[0083] (1) 40 3-day-old PEDV-negative piglets purchased from pig farms that were not immunized with PEDV were fed with artificial milk replacer as experimental piglets and randomly divided into groups Ⅰ, Ⅱ, Ⅲ and Ⅳ Groups, 10 piglets per group, of which, group I is the control group, group II, group III and group IV are probiotic groups;

[0084] (2) After in vitro culture of the probiotics (including Lactobacillus johnsonii and Enterococcus faecium) screened in Example 1, the test piglets were inoculated with probiotics from the morning of the 3rd day in a daily increment of 10 times , Until the experimental piglets grow to 7 days old, the first day of inoculation dose is 5×10 2 cfu / 100uL, and the subsequent daily vaccination dose is 5×10 3 cfu / 100uL, 5×10 4 cfu / 100uL, 5×10 5 cfu / 100uL, 5×10 6 cfu / 100uL; among them, the piglets of group I were inoculated with 100uL of normal saline, the piglets of group Ⅱ were inoculated with 100uL of Lactobacillus johnsonii, th...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a screening method for feeding probiotics against piglet epidemic diarrhea, which comprises the following steps: screening piglets infected with PEDV and disease-resistant piglets and collecting intestinal content samples; piglets infected with PEDV and resistant piglets High-throughput sequencing of intestinal microbial flora of piglets; comparative analysis of intestinal microbial metagenomics between piglets with PEDV infection and disease-resistant piglets; and screening and identification of probiotics against PEDV infection. The screening method for feeding probiotics for anti-epidemic diarrhea of ​​piglets proposed by the present invention specifically screens out probiotics with anti-epidemic diarrhea function from the huge microbial flora of disease-resistant piglets, which is highly targeted and avoids It eliminates the blindness of screening, improves screening efficiency, and saves a lot of manpower, material resources and time.

Description

technical field [0001] The invention relates to the technical field of feed additives for piglets, in particular to a screening method for feeding probiotics against piglet epidemic diarrhea. Background technique [0002] Probiotics are playing an increasingly important role in safeguarding human and animal health or preventing certain diseases, especially under the premise that antibiotics will be completely banned in my country, intestinal health and diarrhea will be bottlenecks that threaten the development of animal husbandry in my country. Among them, porcine epidemic diarrhea caused by porcine epidemic diarrhea virus (Porcine Epidemic Diarrhea Virus, PEDV) is a kind of acute intestinal infectious disease of pigs, and its main clinical symptoms are that feed intake is greatly reduced, watery diarrhea and Accompanied by vomiting, dehydration in severe cases, all ages and breeds can be infected, and it is currently one of the main diseases that cause early death of piglet...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12Q1/6869C12Q1/04
CPCC12Q1/6869C12Q2535/122C12Q2537/165
Inventor 吴涛侯永清丁斌鹰易丹赵迪
Owner WUHAN POLYTECHNIC UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap