Vibrio alginolyticus bacteriophage and bactericidal composition containing same

A technology of vibrio alginolyticus and bacteriophage, which is applied in the direction of bacteriophage, virus/phage, medical raw materials derived from virus/phage, etc., can solve the problems of narrow host spectrum of lysis, short duration of lysis ability, and easy resistance of host bacteria.

Inactive Publication Date: 2018-04-13
SHENZHEN INST OF ADVANCED TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As one of the important weapons to deal with antibiotic resistance, phage has great potential for clinical application, but as far as its current application of single phage therapy is concerned, there are still some potential disadvantages in the application process, such as narrow lytic host spectrum and lytic ability. The duration is short, and the host bacteria are easy to develop resistance, etc. Among them, the host bacteria are easy to develop resistance as the main defect in the application of single phage

Method used

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  • Vibrio alginolyticus bacteriophage and bactericidal composition containing same
  • Vibrio alginolyticus bacteriophage and bactericidal composition containing same
  • Vibrio alginolyticus bacteriophage and bactericidal composition containing same

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Embodiment

[0092] The present invention separates and obtains 6 strains of pathogenic Vibrio from its focus by collecting diseased fish and shrimp samples from aquaculture plants. The screening flow chart of the phage of the present invention is as follows: figure 1 shown. The pathogenicity of the isolated Vibrio was confirmed through field experiments, and 5 of them were determined to be extremely pathogenic, LD 50 4 CFU / mL, action time figure 2 shown.

[0093] Through the plate spot method, according to the presence or absence of transparent spots, the host spectrum of the Vibrio phage under 29 strains of different genus was measured (Table 1), and it was concluded that the phage had strong host specificity and was a Vibrio phage. A specific split phage.

[0094] Table 1 host spectrum

[0095]

[0096]

[0097]

[0098] Remarks: "+" means it can be lysed, "-" means it can't be lysed; the above strains are all isolated from aquaculture fish and shrimp animals and aquacultur...

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Abstract

The invention relates to a Vibrio alginolyticus bacteriophage and a bactericidal composition containing the same. The bacteriophage can rapidly inhibit the growth of Vibrio alginolyticus in a short period of time and is long in the duration of inhibitory effect. The bactericidal composition containing the bacteriophage is safe and effective, has strong specificity and low production cost, and makes up for the deficiencies of single-bacteriophage therapy during the control of pathogenic bacteria.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a Vibrio alginolyticus phage and a bactericidal composition containing the phage. Background technique [0002] my country is a big country in aquaculture. In recent years, my country's aquaculture industry has developed rapidly. With the increasing scale of marine aquaculture and the continuous promotion of intensive farming methods, the frequent occurrence of fish diseases poses a serious threat to the healthy development of large-scale marine fish farming. Fish pathogens mainly include bacteria, viruses, parasites, etc. Among them, bacterial diseases are a type of disease that is more harmful to fish. [0003] Vibrio is a short rod-shaped spore-shaped bacterium that can move. It is widely distributed in seawater and organisms in coastal and estuary sea areas. It is one of the most common bacterial groups in the ocean, and it is also the most important pathogenic bacteria that ca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/00A61K35/76A61P31/04C12R1/92
CPCA61K35/76C12N7/00C12N2795/00021C12N2795/00032A61K2300/00Y02A50/30
Inventor 马迎飞陈玲樊继强
Owner SHENZHEN INST OF ADVANCED TECH
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