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Immobilization method of wine yeast for increasing anthocyanin content in mulberry fruit wine

A wine yeast and immobilization method technology, applied in the field of wine yeast immobilization, can solve the problems of fruit wine anthocyanin content retention rate report, no increase in mulberry anthocyanin content, etc., to improve health care value, increase retention rate, and increase economic benefits Effect

Active Publication Date: 2020-04-24
HUAIYIN INSTITUTE OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many patents for the immobilization of Saccharomyces cerevisiae, such as the patent "A method for immobilizing rice wine brewing yeast (ZL200910136108.2)", "Method for producing kiwi fruit wine with immobilized yeast (ZL201210580516.9)", etc. For the purpose of fermentation efficiency and the production of special fruit wine, there is no report on the retention rate of anthocyanin content in fruit wine
At the same time, the immobilization also binds the yeast to a certain extent, forming a relatively simple reaction environment, and can resist the influence of complex environmental factors, so that its service life is significantly prolonged. Report on Anthocyanin Content

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach 1

[0022] In parts by weight, take 90 parts of sodium alginate, 5 parts of attapulgite clay and 5 parts of edible fungus chaff to prepare a suspension of sodium alginate, attapulgite clay and edible fungus chaff with a mass concentration of 10%, and heat to 45°C Solubilization, after the suspension is cooled, take 100ml and add it to the activated solution (according to the inoculation ratio of 20% during activation, it can be inserted into the aqueous solution containing 20% ​​sugar content at 37°C for 4~6h). The mass concentration is 100g / L, Wine yeast (Anqi Company) solution with a volume of 20ml and mix well to obtain a mixed solution, and then drop the mixed solution into CaCl with a mass concentration of 5.65 g / L 2 In the solution, uniform wine yeast microspheres with a diameter of about 0.5 cm were prepared, and finally the wine yeast microspheres were placed in AlCl with a mass concentration of 4%. 3 The solution was cross-linked for 4 hours, and then washed with sterile ...

Embodiment approach 2

[0024] In parts by weight, take 80 parts of sodium alginate, 10 parts of attapulgite clay and 10 parts of edible fungus chaff to prepare a suspension of sodium alginate, attapulgite clay and edible fungus chaff with a mass concentration of 8%, and heat to 45°C To promote dissolution, after the suspension is cooled, take 120ml and add it to the activated solution (according to the inoculation ratio of 20% during activation, it can be inserted into the aqueous solution containing 20% ​​sugar content at 37°C for 4~6h). The mass concentration is 100g / L, Wine yeast (Anqi Company) solution with a volume of 20ml and mix well to obtain a mixed solution, and then drop the mixed solution into CaCl with a mass concentration of 6.0g / L 2 In the solution, uniform wine yeast microspheres with a diameter of about 0.5 cm were prepared, and finally the wine yeast microspheres were placed in AlCl with a mass concentration of 5%. 3 After cross-linking in the solution for 5 hours, and then washing...

Embodiment approach 3

[0026] In parts by weight, take 70 parts of sodium alginate, 15 parts of attapulgite clay and 15 parts of edible fungus chaff to prepare a suspension of sodium alginate, attapulgite clay and edible fungus chaff with a mass concentration of 5%, and heat to 45°C Solubilization, after the suspension is cooled, take 150ml and add it to the activated solution (according to the inoculation ratio of 20% during activation, it can be inserted into the aqueous solution containing 20% ​​sugar content at 37°C for 4~6h). The mass concentration is 100g / L, Wine yeast (Anqi Company) solution with a volume of 20ml and mix well to obtain a mixed solution, and then drop the mixed solution into CaCl with a mass concentration of 5.0g / L 2 In the solution, uniform wine yeast microspheres with a diameter of about 0.4 cm were prepared, and finally the wine yeast microspheres were placed in AlCl with a mass concentration of 3%. 3 After cross-linking in the solution for 6 hours, and then washing with st...

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Abstract

The invention relates to the field of food fermentation, and discloses a wine yeast fixing method for increasing the anthocyanin content in mulberry fruit wine, comprising the following steps: S1: preparing a suspension of sodium alginate, attapulgite clay and edible fungus chaff ; S2: Add activated wine yeast liquid to the suspension and mix to obtain a mixture; S3: Drop the mixture into CaCl 2 In the solution, immobilized wine yeast microspheres were obtained. The present invention can reduce the degradation of anthocyanin substances in mulberry by Saccharomyces cerevisiae only by immobilizing Saccharomyces cerevisiae, and significantly improve the retention rate of anthocyanins in mulberry fruit wine.

Description

technical field [0001] The invention relates to the field of food fermentation, in particular to a method for immobilizing wine yeast for increasing the anthocyanin content in mulberry fruit wine. Background technique [0002] my country is an ancient country with a long history of sericulture. The production techniques and cultural traditions of planting mulberry and sericulture are the treasures of the Chinese nation. The fruit of the mulberry tree, mulberry, is a good fruit for diet and health preservation. It is also used in the treatment of various diseases in the classics of traditional Chinese medicine. It has been included in the list of both medicine and food announced by the former "Ministry of Health" of our country. At present, the mulberries produced by most of the mulberry varieties planted in China are purple-black fruits, and the material basis for their coloring is anthocyanins. This water-soluble natural pigment is also the root, stem, leaf, flower, etc. of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/14C12N11/10C12N11/04C12R1/865
CPCC12N11/04C12N11/10C12N11/14
Inventor 杨荣玲赵祥杰王朝宇赵玉萍罗洪镇贾建波杨文君
Owner HUAIYIN INSTITUTE OF TECHNOLOGY