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Method for detecting homocysteine and cysteine in rat blood plasma

A technology of homocysteine ​​and cysteine, which is applied in the detection field of rat plasma homocysteine ​​and cysteine, can solve the problems of unpromotable, cumbersome operation, cumbersome methods, etc., and achieve the detection process Simple and convenient, fast detection, good sensitivity

Inactive Publication Date: 2018-04-13
CHONGQING MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the commonly used method for the determination of homocysteine ​​and cysteine ​​is the isotope method, but this method is cumbersome and has radioactive pollution, which is not suitable for wide application
GC-MS can simultaneously determine homocysteine ​​and other substances. Although this method has the advantages of good sensitivity and specificity, the equipment is expensive and cannot be popularized.
HPLC-FLD is also used to determine homocysteine. Since this method needs to be derivatized with o-phthalaldehyde, and the effect of thiol on derivatization needs to be blocked before derivatization, the operation of this method is too cumbersome

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  • Method for detecting homocysteine and cysteine in rat blood plasma
  • Method for detecting homocysteine and cysteine in rat blood plasma
  • Method for detecting homocysteine and cysteine in rat blood plasma

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Embodiment Construction

[0041] The specific implementation of the present invention will be further described below in conjunction with the examples. The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention.

[0042]

[0043] 1 instrument

[0044] High performance liquid chromatography (Shimadzu)

[0045] 2 reagents

[0046] Water: ultrapure water (through 0.45μm water microporous membrane).

[0047] Methanol: American Tiandi HPLC methanol (passed through 0.45μm organic microporous membrane).

[0048] Sodium acetate-sodium octane sulfonate solution: 30 mmol / L sodium acetate and 100 μmol / L sodium octane sulfonate were prepared into 1 L of aqueous solution, pH=5.02 (passed through a 0.45 μm aqueous microporous membrane).

[0049] Standards: homocysteine ​​(sigma-aldrich), cysteine ​​(Aladdin), vanillic acid (Aladdin).

[0050] 3 detection method

[0051] (1) Preparation of standards and samples:

[0052] 1a) Preparati...

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Abstract

The invention discloses a method for detecting homocysteine and cysteine in rat plasma. The method comprises the following steps: 1, preparing a standard substance; 2, reducing a sample with sodium borohydride; 3, removing proteins from the plasma sample; 4, carrying out HPLC-ECD separation determination on the homocysteine and cysteine in the plasma sample; and 5, calculating a result. The method for detecting homocysteine and cysteine in rat plasma has the advantages of simple steps, good accuracy and good sensitivity, and can realize the rapid detection of the homocysteine andcysteine in the sample.

Description

technical field [0001] The invention relates to the field of medicinal chemistry detection, more specifically, the invention relates to a method for detecting homocysteine ​​and cysteine ​​in rat plasma. Background technique [0002] Homocysteine ​​is a thiol-containing amino acid, which mainly exists in the following forms in the body, homocysteine ​​free state, homocysteine ​​bimolecular aggregation into disulfide, homocysteine ​​and protein Binding, and in normal human plasma, 70% homocysteine ​​is bound to protein. Homocysteine ​​mainly comes from methionine ingested in food. It is an intermediate product in the metabolic process of methionine and cysteine, and it does not participate in protein synthesis. Methionine and homocysteine ​​are interconvertible. Homocysteine ​​can interact with betaine, an intermediate metabolite of choline, that is, betaine acts as a methyl donor, and homocysteine ​​and betaine are formed under the action of betaine homocysteine ​​methyltr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/62G01N30/86
CPCG01N30/06G01N30/62G01N30/8634
Inventor 蒋心惠黄丹杜婷婷
Owner CHONGQING MEDICAL UNIVERSITY