A method for separating and purifying suberone from sweet tea old leaves

Abstract

The invention relates to the technical field of separation and purification of natural products, in particular to a method for extracting and isolating suberin from sweet tea old leaves, comprising the following preparation steps: (1) taking sweet tea old leaves as raw materials, drying and dehydrating, and pulverizing to obtain tea powder (2) Take some tea powders from step (1) and add them to 95% ethanol for constant temperature extraction; after suction filtration, repeat the extraction of the filter residue three times, and combine the extracts; (3) Concentrate the extracts in step (2) under reduced pressure until there is no alcohol smell, the ethanol extract of sweet tea old leaves is obtained; (4) the extract of step (3) is dispersed in distilled water, and extracted three times with an equal amount of petroleum ether. Merge the petroleum ether extracts, concentrate under reduced pressure and distill the petroleum ether to obtain the petroleum ether phase extract; (5) take the petroleum ether phase extract from step (4) and subject it to silica gel column chromatography, using a certain volume ratio of petroleum ether and ethyl acetate Perform gradient elution to collect effective fractions; (6) Concentrate the effective fractions in step (5) under reduced pressure to remove the solvent, recrystallize and dry to obtain pure suberone. The method for extracting, separating and purifying suberin is simple and easy, has high separation efficiency, low energy consumption and less pollution, and opens up a new way for the utilization of old sweet tea leaves.

Description

technical field [0001] The invention relates to the technical field of separation and purification of natural products, and mainly relates to a method for purifying suberone from old sweet tea leaves. Background technique [0002] Friedelin (Friedelin) is a typical triterpenoid, which belongs to the triterpenoid pentacyclic triterpenes. It is a white needle-like crystal with a melting point of 263-263.5°C. It can be dissolved in organic solvents such as chloroform and petroleum ether, but insoluble in water. Suberone has a wide range of biological activities and medical applications, such as anti-inflammatory, analgesic, antioxidant, free radical scavenging and good inhibition of pathogenic bacteria and fungi. Suberone also has insecticidal effect and can be applied to plant protection. Suberone exists in many plants, such as the whole plant of Euonymus euonymus, the stem of Euphorbiaceae Chongyang wood, and the branches of Litmus sparrow in Litmus. [0003] Sweet tea, al...

AI Technical Summary

Problems solved by technology

However, since the finished sweet tea leaves only need young leaves that have sprouted soon, a large number of old leaves with older leaf ages cannot be utilized.