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Production method of freeze-dried liquid strain for Auricularia auricula-judae

A production method and technology for strains, which are applied in the production field of liquid freeze-dried strains of fungus, can solve the problems of difficult storage and transportation of liquid strains, easy to be polluted by miscellaneous bacteria, low labor efficiency, etc., and achieve high popularization and application value, The effect of more germination points and shorter fruiting cycle

Inactive Publication Date: 2018-04-17
宁波市纯韩花漫生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The strains used in the artificial cultivation of fungus are mostly prepared by the solid fermentation method, but this method has a long production cycle, high labor intensity, low labor efficiency, and is easily polluted by miscellaneous bacteria. Compared with solid strains, liquid strains have a shorter cycle, The advantages of uniform bacterial age, fast growth, convenient inoculation, and less pollution are the development direction of edible fungus seed production in the future
However, liquid strains also have the disadvantages of being difficult to store and transport, which limits the application of liquid seed production technology in the field of edible fungus cultivation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Under sterile conditions, the fungus mother species were cultured in the slant medium, the culture conditions were: temperature 24°C, time 5 days, until the mycelia covered the slant; the slant mycelium was transplanted to the liquid culture medium of the shaker flask to statically After one day of cultivation, shake culture amplification is carried out. The culture conditions are: temperature 25°C, shaker speed 100r / min, liquid volume 100ml / 500ml Erlenmeyer flask, culture period 4 days; Inoculate 5% of the inoculum into a fermenter for culture and amplification. The culture conditions are: temperature 25°C, rotation speed 100r / min, ventilation rate 0.02Mpa, and a culture period of 4 days; the mature bacterial liquid cultured in the fermenter is centrifuged or Collect mycelium balls by filtration, and crush the mycelium balls; add freeze-drying protective agent to the broken mycelium, and pre-freeze in the refrigerator for 0.5 hours; put the pre-frozen mycelium in a free...

Embodiment 2

[0028] Under sterile conditions, the fungus female species were cultured in the slant medium, the culture conditions were: temperature 32°C, time 8 days, until the mycelia covered the slant; the slant mycelium was transplanted into the shake flask liquid medium to statically Place the culture for 2 days and then carry out shaking culture amplification. The culture conditions are: temperature 32°C, shaker speed 200r / min, liquid volume 180ml / 500ml Erlenmeyer flask, culture period 7 days; the cultured shake flask strains Inoculate 10% of the inoculum into a fermenter for culture and amplification. The culture conditions are: temperature 32°C, rotation speed 180r / min, air flow 0.1Mpa, and a culture period of 7 days; the mature bacterial liquid cultured in the fermenter is centrifuged or Collect mycelium balls by filtration and crush them; add freeze-drying protective agent to the broken mycelium, and pre-freeze in the refrigerator for 2 hours; put the pre-frozen mycelium in a freez...

Embodiment 3

[0034] Under sterile conditions, the fungus mother species were cultivated in the slant medium, the culture conditions were: temperature 28°C, time 7 days, until the mycelium covered the slant; the slant mycelium was transplanted into the liquid medium of the shaker flask to statically Place the culture for 1.5 days and then carry out shaking culture amplification. The culture conditions are: temperature 29°C, shaker speed 150r / min, liquid volume 140ml / 500ml Erlenmeyer flask, culture period 5 days; the cultured shake flask strains Inoculate 8% of the inoculum into a fermenter for culture and amplification. The culture conditions are: temperature 29°C, rotation speed 140r / min, air flow 0.06Mpa, and a culture period of 5 days; Filtration treatment collects mycelium balls, and crushes mycelium balls; adds freeze-drying protective agent to the broken mycelia, and pre-freezes in the refrigerator for 1.2 hours; puts the pre-frozen mycelia in a freeze dryer After 19 hours of internal...

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Abstract

The invention discloses a production method of liquid freeze-dried strains of fungus, which belongs to the field of cultivation of edible fungus strains. The steps of the production method are as follows: under aseptic conditions, the mother species of fungus is cultivated in a slant medium, and the hyphae are treated until the slant is covered; transplant the mycelium of the slant into the liquid medium of the shake flask and then carry out shaking culture and amplification; inoculate the cultured shake flask strains into the fermenter for culture and amplification; Centrifuge or filter the bacterial solution to collect mycelium balls, and crush the mycelium balls; add freeze-drying protective agent to the broken mycelium, and pre-freeze in the refrigerator; put the pre-frozen mycelia in After being dried in a freeze dryer, it is divided into blocks to become freeze-dried strains. The mycelial balls formed by the liquid culture part of the present invention are uniform in size, the number of mycelial balls is large, and there are many germination points, rapid germination, and a short fruiting cycle; the liquid freeze-dried bacteria produced by the present invention can be stored for half a year without vigor, and the inoculation is convenient .

Description

technical field [0001] The invention relates to the field of cultivation of edible fungus strains, in particular to a production method of liquid freeze-dried fungus strains. Background technique [0002] The strains used in the artificial cultivation of fungus are mostly prepared by solid fermentation, but this method has a long production cycle, high labor intensity, low labor efficiency, and is easily polluted by miscellaneous bacteria. Compared with solid strains, liquid strains have a short cycle, The advantages of uniform bacterial age, fast growth, convenient inoculation, and less pollution are the development direction of edible fungus seed production in the future. However, liquid strains also have the disadvantages of being difficult to store and transport, which limits the application of liquid seed production technology in the field of edible fungus cultivation. Contents of the invention [0003] The technical task of the invention is to provide a method for p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/04C12R1/645
CPCC12N1/14C12N1/04
Inventor 张永超
Owner 宁波市纯韩花漫生物科技有限公司
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