Common Wheat SNP Locus Marker and Its Application in Detection of Wheat Related Traits
A technology of wheat and loci, which is applied in recombinant DNA technology, microbial measurement/inspection, DNA/RNA fragments, etc., can solve the problems of long genetic distance between markers and functional genes, fewer markers for yield traits, and large wheat genome, etc., to achieve Low cost, increased plant height, good repeatability
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Embodiment 1
[0049] Example 1. Discovery of SNP354 sites and development of detection markers
[0050] 1. Discovery of SNP354 loci
[0051] 1. Wheat material for testing
[0052]30 wheat materials (numbered NO.1-NO.30) distributed in different wheat regions in my country with large differences in yield traits and phenotypes were selected as the excavation materials for polymorphic SNP sites (Table 1).
[0053] 2. Sequence Alignment
[0054] The genomic DNA of the above wheat materials was extracted by conventional CTAB method, and 30 wheat materials were amplified respectively. The amplification primers used were specific primer F and specific primer R.
[0055] Specific primer F: 5'-CTGAACAGGCAGATGACATAAA-3' (SEQ ID NO: 1)
[0056] Specific primer R: 5'-GAAGCAACCGGCCAATGTGAC-3' (SEQ ID NO: 2)
[0057] A PCR amplification product of 1543bp was obtained.
[0058] The PCR amplification products of different tested wheat materials were sequenced, and the nucleotide sequence was sequence ...
Embodiment 2
[0095] Example 2, Establishment of Ta-7A-SNP354 site in the detection of thousand-grain weight, effective tiller, plant height and ear length traits of wheat materials
[0096] The basic information of all wheat micro-core germplasm materials in this example (105 bred varieties and 157 local varieties) were measured in Luoyang, Henan Province in 2002, in Luoyang, Henan Province in 2005, and in Beijing in 2006. When measuring phenotypic data, at least 3 repeated determinations were set for each sample, and the results were averaged.
[0097] Table 2 shows the names of 262 wheat micro-core germplasm materials
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[0107] 1. PCR amplification of specific fragments
[0108] Using the genomes of the tested wheat varieties shown in Table 2 as templates, PCR amplification was performed using a pair of specific primers F and R.
[0109] Ta-7A-SNP354-F: 5'-CTGAACAGGCAGATGACATAAA-3...
Embodiment 3
[0146] Example 3, Ta-7A-SNP354 site in the detection of thousand-grain weight, effective tillering, plant height and ear length traits of wheat materials
[0147] The F1 generation after crossing Zhengmai 9023 and Yanzhan 1, and then selfing the F1 generation to obtain the F4 generation;
[0148] The DNA of 60 strains of the F4 generation was extracted, and the haplotype of the Ta-7A-SNP354 locus of the 60 strains of the F4 generation in 2016-2017 was detected according to the method of Example 2.
[0149] The result is as follows:
[0150] Among the 60 strains of the F4 generation, 26 strains had the haplotype of Ta-7A-SNP354 as GG, 32 strains had the haplotype of Ta-7A-SNP354 as AA, and the unit of Ta-7A-SNP354 There are two strains of type AG (abandoned).
[0151] The phenotype statistics of the above-mentioned each haplotype plants were carried out in the field, and the results are as follows:
[0152] The average thousand-grain weight of Ta-7A-SNP354 haplotype AA was 4...
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