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Compounded preparation for preventing and treating amyloodiniosis of large yellow croakers, and preparation method thereof

A technology of dinoflagellate and large yellow croaker, which is applied in the field of breeding, can solve the problems of unstable effect, chronic poisoning of large yellow croaker that pollutes water, etc., and achieve the effects of no secondary pollution, control of pathogenic reproduction, and high activity

Active Publication Date: 2018-04-20
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods not only have unstable effects, but also pollute water bodies and induce chronic poisoning of large yellow croakers

Method used

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  • Compounded preparation for preventing and treating amyloodiniosis of large yellow croakers, and preparation method thereof
  • Compounded preparation for preventing and treating amyloodiniosis of large yellow croakers, and preparation method thereof
  • Compounded preparation for preventing and treating amyloodiniosis of large yellow croakers, and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Get 4g of DL-malic acid, 6g of maleic acid and 10g of xylitol and 79.6g of distilled water in a 500ml beaker, add a magnetic bar, after mixing uniformly on a magnetic stirrer, add 0.4g of solid acid catalyst (SO 4 2- / SiO 2 -TiO 2 ), using an ultrasonic cell disruptor with the assistance of ultrasound (frequency 20kHz), place the beaker in a water bath at 80°C for 8 hours to prepare raw material 1; take another 250 beaker, add 1g glyceraldehyde, 10g β-1,4-N- Acetyl-D-amino-glucose 8-mer and 89g distilled water at an intensity of 20W / m 2 The reaction was stirred for 10 h under the irradiation of ultraviolet light to prepare raw material 2. After mixing the raw materials 1 and 2 according to the volume ratio of 1:1, stirring continuously for 2 hours, the finished compound preparation is obtained.

[0023] Place 6 4M aquaculture ponds in a mariculture pond with a depth of 3 meters, a maximum water level of 2.5 meters, clean water, no pollution, and an average salinity ...

Embodiment 2

[0030] Get 2 g of DL-malic acid, 7 g of maleic acid, 4 g of xylitol and 86.7 g of distilled water in a 500 ml beaker, add a magnetic rod, mix well on a magnetic stirrer, and then add 0.3 g of a solid acid catalyst (SO 4 2- / SiO 2 -TiO 2 ), using an ultrasonic cell disruptor with the assistance of ultrasound (frequency 50kHz), put the beaker in a water bath at 60°C for 10h to prepare raw material 1; take another 250 beaker, add 0.4g glyceraldehyde, 5g β-1,4-N - Acetyl-D-amino-glucose 8-mer and 94.6g distilled water at an intensity of 10W / m 2 The reaction was stirred for 20 h under the irradiation of ultraviolet light to prepare raw material 2. After mixing the raw materials 1 and 2 according to the volume ratio of 1:1, stirring continuously for 2 hours, the finished compound preparation is obtained.

[0031] Place 6 4M aquaculture ponds in a mariculture pond with a depth of 3 meters, a maximum water level of 2.5 meters, clean water, no pollution, and an average salinity of ...

Embodiment 3

[0038] Get DL-malic acid 2g, maleic acid 3g and xylitol 4g and 90.7 g of water in a 500ml beaker respectively, add a magnetic bar, after mixing uniformly on a magnetic stirrer, in 0.3g solid acid catalyst (SO 4 2- / SiO 2 -TiO 2 ) under the action of an ultrasonic cell disruptor with the assistance of ultrasound (frequency 80kHz), place the beaker in a water bath at 80°C for 8 hours to prepare raw material 1; take another 250 beaker, add 1g glyceraldehyde, 5g β-1,4- N-acetyl-D-amino-glucose 8-mer and 94ml of distilled water at an intensity of 30W / m 2 The reaction was stirred for 15 h under the irradiation of ultraviolet light to prepare raw material 2. After mixing the raw materials 1 and 2 according to the volume ratio of 1:1, stirring continuously for 2 hours, the finished compound preparation is obtained.

[0039] Place 6 4M aquaculture ponds in a mariculture pond with a depth of 3 meters, a maximum water level of 2.5 meters, clean water, no pollution, and an average sal...

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Abstract

The invention relates to a compounded preparation for preventing and treating amyloodiniosis of large yellow croakers, and a preparation method thereof. The compounded preparation comprises DL-malic acid, maleic acid, xylitol, a solid acid catalyst (SO<4><2-> / SiO2-TiO2), glyceraldehyde and beta-1,4-N-acetyl-D-glucosamine octamer. The compounded preparation can induce the gut cells of the large yellow croakers to release a large amount of Amyloodinium Ocellatum cell decomposing enzymes after entering the bodies of the big yellow croakers in order to inhibit the generation of newborn Amyloodinium Ocellatum cells, so the incidence of the amyloodiniosis of the large yellow croakers is effectively controlled; the environmentally-friendly beta-1,4-N-acetyl-D-glucosamine polymer compounded preparation induces the large yellow croakers to produce the Amyloodinium Ocellatum cell decomposing enzymes, inhibits the generation of the newborn Amyloodinium Ocellatum cells, and controls pathogenic breeding; and the compounded preparation has the advantages of good dispersion, easiness in penetration, high activity, safety, reliability and no secondary pollution.

Description

technical field [0001] The invention belongs to the field of breeding technology, and in particular relates to a compound preparation for preventing and treating large yellow croaker amyloid dinoflagellate disease and its preparation. Background technique [0002] In recent years, the water quality of coastal waters and estuaries has been seriously polluted. The incidence of amyloid dinoflagellates in ponds and workshops has remained high. Unisexual, can parasitize a variety of marine fish. Starch dinoflagellates belong to dinoflagellates, dinoflagellates and genus Dinoflagellates in terms of classification, and some people classify it into the phylum Sarcozoa, Phytoflagellates, Lusoflagellates, Endospermaceae, and the genus Starch dinoflagellates. Therefore, the disease is also called starch egg planarianism. The worms in the parasitic stage are vegetative bodies, with rhizoid protrusions at one end. After the vegetative bodies mature or the diseased fish die, they retrac...

Claims

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Application Information

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IPC IPC(8): A61K31/716A61K31/225A61P33/14
CPCA61K31/225A61K31/716A61K2300/00
Inventor 葛亚明刘俊稚韩厚峰张航夏松养
Owner ZHEJIANG OCEAN UNIV