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Stevioside extraction and purification method

A technology of stevioside and purification method, applied in the field of stevioside, can solve problems such as difficulty in purification and separation, and achieve the effects of simple process, reduced cost and excellent adsorption capacity

Inactive Publication Date: 2018-04-20
BENGBU HUADONG BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the existing extraction process of steviol glycosides, while extracting steviol glycosides, a large amount of impurities are also extracted, making purification and separation difficult

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A method for extracting and purifying steviol glycosides, comprising the steps of:

[0026] S1. Grind 60g of stevia dry leaves, add 500g of water to soak for 20min, add 0.2g of cellulase for enzymolysis for 20h, and obtain an enzymatic hydrolysis solution;

[0027] S2. Adjust the pH value of the enzymolysis solution to 5.6-6.5, heat up to 55° C. for leaching for 2 hours, cool, settle and centrifuge at 2400 r / min, and filter to obtain the leaching solution;

[0028] S3. Mix 10g of jute stalk, 6g of zeolite, 2g of palygorskite, 3g of rectorite, and 1g of bentonite evenly, calcinate at 580°C for 15min, drop to room temperature, pulverize, add 30g of water, mix and grind for 30min, and spray dry to obtain a composite adsorbent;

[0029] S4. Heat 300g of the extract in a water bath, the temperature of the water bath is 75°C, add 1g of flocculant and stir evenly, adjust the pH value of the system to 7.5-8.2, flocculate for 10min, centrifugally settle at a centrifugal speed of...

Embodiment 2

[0032] A method for extracting and purifying steviol glycosides, comprising the steps of:

[0033] S1. Crush 80g of stevia dry leaves, add 300g of water to soak for 40min, add 0.1g of cellulase for enzymolysis for 60h, and obtain an enzymolysis solution;

[0034] S2. Adjust the pH value of the enzymolysis solution to 5.6-6.5, heat up to 45° C. for extraction for 4 hours, cool, settle and centrifuge at a centrifugal speed of 2000 r / min, and filter to obtain the extraction solution;

[0035] S3. Mix 20g jute stalk, 2g zeolite, 4g palygorskite, 1g rectorite, and 3g bentonite evenly, calcinate at 520°C for 25min, cool down to room temperature, pulverize, add 20g of water, mix and grind for 60min, and spray dry to obtain a composite adsorbent;

[0036] S4. Heat 200g of the extract in a water bath, the temperature of the water bath is 85°C, add 0.5g of flocculant and stir evenly, adjust the pH value of the system to 7.5-8.2, flocculate for 20min, centrifuge and settle at a centrifug...

Embodiment 3

[0039] A method for extracting and purifying steviol glycosides, comprising the steps of:

[0040] S1. Grind 65g of stevia dry leaves, add 450g of water to soak for 25min, add 0.18g of cellulase for enzymolysis for 30h, and obtain an enzymolysis solution;

[0041] S2. Adjust the pH value of the enzymatic hydrolysis solution to 5.6-6.5, heat up to 52°C for extraction for 2.5 hours, cool, settle and centrifuge at a centrifugal speed of 2300r / min, and filter to obtain the extraction solution;

[0042] S3. Mix 12g of jute stalk, 5g of zeolite, 2.5g of palygorskite, 2.5g of rectorite, and 1.5g of bentonite evenly, calcinate at 560°C for 18min, cool down to room temperature, pulverize, add 28g of water, mix and grind for 40min, spray dry to obtain composite adsorption agent;

[0043] S4. Heat 280g of the extract in a water bath, the temperature of the water bath is 78°C, add 0.8g of flocculant and stir evenly, adjust the pH value of the system to 7.5-8.2, flocculate for 12min, cent...

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PUM

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Abstract

The invention discloses a stevioside extraction and purification method, which comprises the following steps of crushing stevia rebaudiana dry leaves; adding water for soaking; adding cellulase for enzymolysis so as to obtain enzymolysis liquid; regulating the pH value of the enzymolysis liquid to be 5.6 to 6.5; raising the temperature for digestion; performing cooling, sedimentation centrifugation and filtering to obtain digestion liquid; uniformly mixing jute stalks, zeolite, palygorskite, rectorite and bentonite; performing roasting; lowering the temperature; performing crushing; adding water for mixed milling; performing spray drying to obtain composite adsorbents; performing water bath heating on the digestion liquid; adding flocculating agents; performing uniform stirring; regulatingthe system pH value to be 7.5 to 8.2; performing flocculating treatment, centrifugal sedimentation and filtering; adding composite adsorbents; performing boiling in a normal pressure backflow state;performing filtering and concentration to obtain concentration liquid; feeding the concentration liquid onto a silica gel chromatography column; then, performing elution by a mixed solvent; collectingfractions; performing vacuum concentration and vacuum drying to obtain the stevioside.

Description

technical field [0001] The invention relates to the technical field of steviol glycosides, in particular to a method for extracting and purifying steviosides. Background technique [0002] Stevia is native to Paraguay and is a plant belonging to the sugar family. The temperature during the entire growth period is above 20°C, and it is thermophilic. In 1970, Japan introduced stevia from Brazil, began domestication, cultivation, and glycoside production, and at the same time conducted toxicology, food inspection and other tests, and first developed and utilized stevia products - steviol glycosides. [0003] Steviol glycosides are a class of tetracyclic diterpenoid derivatives that mainly exist in the leaves of Stevia rebaudiana, mainly including eight kinds of glycosides, including steviol glycosides, rebaudioside A, rebaudioside B, and rebaudioside C. The density is about 300 times that of sucrose. Studies have shown that steviol glycosides have the characteristics of high ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/256C07H1/08
CPCC07H15/256C07H1/08
Inventor 邢飞邢伟张志华庞正银
Owner BENGBU HUADONG BIOLOGICAL TECH
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