Familial deteriorating atrioventricular conduction block gene diagnosis kit

A technique for atrioventricular block and gene diagnosis, applied in the field of molecular biology, can solve the problems of inability to meet the requirements of sequencing, low efficiency, and high cost of single nucleotide sequencing, to increase the clinical detection rate and accuracy, The effect of reducing cost and improving detection accuracy and stability

Inactive Publication Date: 2018-04-20
FUWAI HOSPITAL CHINESE ACAD OF MEDICAL SCI & PEKING UNION MEDICAL COLLEGE
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Problems solved by technology

[0003] As a traditional sequencing technology, Sanger sequencing can only determine one base sequence in one reaction. Although it is accurate, it is inefficient and the cost of single nucleotide sequencing is high.
For the research of compl

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  • Familial deteriorating atrioventricular conduction block gene diagnosis kit
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  • Familial deteriorating atrioventricular conduction block gene diagnosis kit

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Embodiment Construction

[0032] The following embodiments are only examples of the practical application of the present invention, and the practical application of the present invention is not limited to the following embodiments:

[0033] 1. The following is a further detailed and complete description of the present invention by way of examples, but the present invention is not limited to the scope of the examples.

[0034] 2. Use TIANamp Blood DNA Midi Kit (DP332) to extract the genome of the sample to be tested. The volume of the elution buffer (TE) is not less than 200μL, and the OD260 / 280 value reaches 1.8~2.0. Take 2μL of 25ng / μL DNA as a starting point Start template.

[0035] 3. Use Qubit to quantify the sample. After quantification, dilute the g DNA sample to 25ng / μL with ddH2O.

[0036] 4. Build a library

[0037] 5. DNA fragmentation of genomic samples and adding adapters and tags

[0038] 6. Take 1.5ml Ethanol and add it to 4.5ml TargetCap Stop Solution and mix well to make the final concentration o...

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Abstract

The invention discloses a familial deteriorating atrioventricular conduction block gene diagnosis kit, and is characterized in that the invention relates to the diagnosis kit for detecting familial deteriorating atrioventricular conduction block genes by a large-scale parallel sequencing platform NGS technique. The kit is mainly characterized in that: A, a capture probe is uniquely designed and prepared and aims at all exon fragments of genes SCN5A and TRPM4; B, a connector with a label sequence is uniquely designed; C, a probe sequence is subjected to PCR amplification by universal primers; and D, a uniquely designed target fragment after mixing is subjected to capture operation.

Description

Technical field [0001] The invention belongs to the field of molecular biology, relates to medical diagnosis and biotechnology, relates to an in vitro diagnostic kit for familial deteriorating atrioventricular block gene mutations, and is a family of NGS technology applied to a new generation sequencing platform Gene diagnosis kit for sexually worsening atrioventricular block. Background technique [0002] Heart block is a permanent or temporary impulse conduction disorder caused by anatomy or malfunction. It can be divided into 3 degrees according to its severity. Common causes of complete atrioventricular block are drug action, hypertonic vagus nerve, coronary heart disease, electrolyte imbalance, endocarditis, tumor, mucinous edema, calcified aortic stenosis, polymyositis, myocardial infiltrating disease And open heart surgery. In adults, drug poisoning, coronary heart disease and degenerative changes are the most common causes. Coronary atherosclerosis is the primary cause...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883C12Q1/6869
CPCC12Q1/6869C12Q1/6883C12Q2600/156
Inventor 王继征宋雷惠汝太邹玉宝张禅那
Owner FUWAI HOSPITAL CHINESE ACAD OF MEDICAL SCI & PEKING UNION MEDICAL COLLEGE
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