Acid degradation method of Hericium erinaceus active polysaccharide
A technology of Hericium erinaceus polysaccharides and acid degradation, which can be applied to organic active ingredients, pharmaceutical formulations, allergic diseases, etc. It can solve the problems of undiscovered polysaccharide derivatives, achieve immune activity, good solubility, and increase the amount of release. Effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0038] Embodiment 1: screening of acid species
[0039] Weigh 4 parts of 100mg macromolecule Hericium ericaceus β-glucan H6PC20 and place them in stoppered test tubes, add water to dissolve, and make a 2g / L solution; then add citric acid, hydrochloric acid, sulfuric acid, trifluoroacetic acid , the final concentration of acid is 0.5mol / L. Place the stoppered test tube in a water bath at 100°C and hydrolyze for 2 hours. After complete hydrolysis, the acidic polysaccharide solution is immediately cooled in an ice bath, and then neutralized with 1M NaOH solution to adjust the pH to neutral. Centrifuge at 12000×g for 15 minutes to remove impurities. The centrifuged supernatant was dialyzed with a 3500Da dialysis bag to remove the acid. The acid hydrolyzate was obtained by freeze-drying, and the molecular weight distribution diagrams of the macromolecular Hericium erinaceus polysaccharide H6PC20 after acid hydrolysis were respectively determined by high performance liquid chromat...
Embodiment 2
[0042] Embodiment 2: Determination of trifluoroacetic acid degradation time
[0043] Take by weighing 5 parts of 100mg Hericium β-glucan H6PC20 and put into stopper test tube respectively, add water to dissolve, be made into the solution of 1g / L; Every tube adds trifluoroacetic acid solution, makes the final concentration of trifluoroacetic acid be 0.5mol / L, seal the tube, place it in a water bath at 90°C, and react for 30min, 50min, 70min, 90min and 110min respectively. After the reaction, cool down and centrifuge at 8000×g for 20 minutes to remove the precipitate. The centrifuged supernatant is dialyzed with a 3500Da dialysis bag to remove trifluoroacetic acid and vacuum freeze-dried to obtain the degraded Hericium erinaceus polysaccharide fragment. The molecular weights of different polysaccharide fragments were determined by high performance liquid chromatography, and the activity of different molecular weight Hericium erinaceus polysaccharides stimulating macrophage RAW26...
Embodiment 3
[0046] Embodiment 3: Determination of the concentration of trifluoroacetic acid
[0047] Weigh 5 parts of 100 mg of Hericium erinaceus β-glucan H6PC20 into stoppered test tubes respectively, add water to dissolve, and make a 2 g / L solution. Trifluoroacetic acid solutions with final concentrations of 0.1, 0.3, 0.5, 0.7, and 1.0 mol / L were added respectively, the tube was sealed, and placed in a water bath at 90°C for 50 minutes to react. After the reaction, cool down, centrifuge at 12000×g for 20 min to remove the precipitate, take the supernatant and dialyze it with a 3500Da dialysis bag to remove trifluoroacetic acid, and vacuum freeze-dry to obtain the degraded Hericium erinaceus polysaccharide fragment. The molecular weights of different polysaccharide fragments were determined by high performance liquid chromatography, and the activity of different molecular weight Hericium erinaceus polysaccharides stimulating macrophage RAW264.7 to release NO in vitro was determined.
...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More