Pharmaceutical preparation for chronic pelvic inflammatory disease and preparation method thereof
A technology for chronic pelvic inflammatory disease and pharmaceutical preparations, which is applied in the direction of medical formulas, drug combinations, and medical preparations containing active ingredients, and can solve problems such as high cost, many types of raw materials, and unclear mechanism of action
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Embodiment 1
[0034] A pharmaceutical preparation for chronic pelvic inflammatory disease, which is prepared from the following raw materials:
[0035] Smilax 400g, Salvia 200g, Prunella vulgaris 200g, Dandelion 250g, Carnation 2000g, Rhizoma 150g, Eucommia 150g, Panax notoginseng 50g, Chuanxiong 80g.
[0036] The preparation method of described pharmaceutical preparation comprises the steps:
[0037]Step 1): take Danshen, crush it into 200 mesh powder, impregnate it with 1.5 times the weight of 90% (v / v) ethanol for 24 hours, then add 90% (v / v) ethanol solution for percolation, and the flow rate is 1-3ml / min, collect the percolation liquid, obtain the medicinal liquid A after the percolation liquid reclaims ethanol, and the medicinal residue is for subsequent use;
[0038] Step 2): Take Rhizoma Chuanxiong, Eucommia, Panax notoginseng and Carnation, mix and grind them into 200-mesh powder, add 70% (v / v) ethanol 5 times the weight of the crude drug each time for impregnation for 24 hours, ...
Embodiment 2
[0046] Embodiment 2 Zoology experiment
[0047] Establish mouse animal model of pelvic inflammatory disease: Select healthy Kunming strain mice to construct mouse pelvic inflammatory disease model. Inject 4% chloral hydrate intraperitoneally according to 1% of the mouse's body weight, general anesthesia, place the mouse on a fixed table after a few minutes, subtract the abdominal hair, sterilize the skin with 75% ethanol, take sterilized scissors and tweezers, and cut it open The skin and muscle layer of the mouse’s lower abdomen were cut about 1 cm to expose the uterus. A sterile needle was used to puncture the middle of the uterine horn on one side of the uterus for about 5-6 needles. After the completion, the abdomen was closed layer by layer and the abdominal operation area was disinfected.
[0048] Experimental procedure: select the mice of above-mentioned modeling, be divided into blank control group (do not do any processing) at random after 7 days, sham operation group...
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