Tetranitromacrocyclic manganese complex modified chitosan composite material with high SOD enzyme activity and preparation method thereof

A composite material, chitosan technology, applied in the field of superoxide dismutase simulants, can solve the problems of unevenness, poor dispersion of nanoparticles, easy agglomeration, etc., and achieve good biocompatibility

Active Publication Date: 2020-08-28
WUHAN INSTITUTE OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, nanoparticles have poor dispersion, are uneven and easy to agglomerate; synthetic polymers have many problems in degradation rate, toxicity and emission of degraded products, and natural materials with good biocompatibility, biodegradability and non-toxic degradation products such as Carboxymethyl cellulose and chitosan attract people's attention

Method used

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  • Tetranitromacrocyclic manganese complex modified chitosan composite material with high SOD enzyme activity and preparation method thereof
  • Tetranitromacrocyclic manganese complex modified chitosan composite material with high SOD enzyme activity and preparation method thereof
  • Tetranitromacrocyclic manganese complex modified chitosan composite material with high SOD enzyme activity and preparation method thereof

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Embodiment 1

[0030] Synthetic route of the present invention: take 1,4,7,10-tetraazacyclododecane (Cyclen) as raw material to react with chloroacetic acid to obtain a tetraazine macrocyclic compound (DOTA) containing four carboxyl cantilevers, and DOTA As a raw material, it reacts with manganese acetate and chitosan under the catalysis of EDC 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and NHS (N-hydroxysuccinimide) Synthesis of chitosan gel modified with manganese complex of DOTA by amidation reaction

[0031] 1) The preparation method of chitosan gel, 1.5g of 95% deacetylated chitosan was dissolved in 500mL of 1% (m / v) acetic acid solution, stirred until completely dissolved and left standing for one hour to make it stable. Afterwards, the chitosan solution was pumped into 500 mL 0.5 mg / mL TPP (sodium tripolyphosphate) solution via a peristaltic pump at a rate of 100 mL / min, and vigorously magnetically stirred. After the dropwise addition was completed, the vigorous stir...

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Abstract

The invention discloses a chitosan composite material modified by a tetraaza macrocyclic manganese complex with high SOD (Super Oxide Dismutase) enzyme activity and a preparation method thereof. A polyaza macrocyclic complex is a complex formed by a macrocyclic tetraaza compound containing four cantilevers and manganese ions; the complex is formed through combining a carboxyl group and an amino group of chitosan through an amide bond. According to the chitosan composite material disclosed by the invention, the tetraaza macrocyclic manganese complex with the high SOD is combined with chitosan through a covalent bond, so that active sites are uniformly dispersed on a chitosan carrier; the chitosan composite material has the characteristics of high stability, good activity and good biocompatibility and is hopefully applied to clinical medicine and medicines and an application field of related problems of eliminating superoxide anion free radicals.

Description

technical field [0001] The invention relates to a superoxide dismutase (SOD) mimic, in particular to a preparation method of the SOD mimic with chitosan as a carrier and a polynitrogen macrocycle complex as an active site. Background technique [0002] Superoxide dismutase is an important antioxidant enzyme in biological systems. Antioxidants with SOD function can be used in medicine for the treatment of Alzheimer's disease, stroke, heart disease, aging and cancer. The development of SOD Mimics of antioxidant enzyme activity, understanding their efficient catalytic mechanism and developing potential therapeutic antioxidant compounds are of great significance for improving lifespan, delaying aging, treating and preventing related diseases caused by ROS. In order to obtain new SOD mimics with high activity, good biocompatibility, long circulation period in vivo, non-toxicity and specific anti-oxidation effect, appropriate macromolecular materials must be selected as carriers t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/08
CPCC08B37/003
Inventor 周红瞿隽申潘志权程清蓉
Owner WUHAN INSTITUTE OF TECHNOLOGY
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