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Micro-fluidic cell sorting chip and method coupled with dielectrophoresis and spatial isolation

A space separation and microfluidic technology, applied in the field of cell sorting, can solve the problems of large chip size, etc., and achieve the effect of solving the problem that the chip channel is too long and the dielectric difference of different cells is not obvious

Inactive Publication Date: 2018-05-01
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage is that the chip channel needs to be long enough to achieve an ideal sorting effect, so the chip size is large

Method used

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  • Micro-fluidic cell sorting chip and method coupled with dielectrophoresis and spatial isolation
  • Micro-fluidic cell sorting chip and method coupled with dielectrophoresis and spatial isolation
  • Micro-fluidic cell sorting chip and method coupled with dielectrophoresis and spatial isolation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A microfluidic cell sorting chip coupled with dielectrophoresis and space separation in this embodiment, its structure schematic diagram is as follows figure 1 shown. It consists of a glass substrate ①, a main channel ② on the glass substrate and electrodes ③ on both sides of the main channel. The main channel contains 8 contraction and expansion structures, and one electrode with a thickness of 100 um is arranged on both sides of the main channel. The height of the main channel is 100um, the length is 8mm, the width of the shrinkage part of the channel is 200um and the width of the expansion part is 600um, and the shape of the expansion part on the side of the channel is a semicircle with a radius R of 200um. The schematic diagram of its structural size is as follows figure 2 shown.

[0033] The microfluidic cell sorting chip of this example was prepared by the following method:

[0034] Using conductive carbon ink as the material, microelectrodes (electrode thickn...

Embodiment 2

[0039] A microfluidic cell sorting chip coupled with dielectrophoresis and space separation in this embodiment is composed of a glass substrate, a main channel on the glass substrate, and electrodes on both sides of the main channel. The main channel contains 8 contraction and expansion structures, and one electrode with a thickness of 130um is arranged on both sides of the main channel. The length of the main channel is 8 mm, the height is 130 um, the width of the constricted part of the channel is 200 um and the width of the expanded part is 600 um, and the shape of the expanded part on one side of the channel is a semicircle with a radius of 200 um.

[0040] The microfluidic cell sorting chip of this example was prepared by the following method:

[0041] Using conductive carbon ink as the material, microelectrodes (electrode thickness 130um) were prepared on the float glass substrate by screen printing; using polydimethylsiloxane (PDMS) as the material, micro-machining (MEM...

Embodiment 3

[0046] A microfluidic cell sorting chip coupled with dielectrophoresis and space separation in this embodiment is composed of a glass substrate, a main channel on the glass substrate, and electrodes on both sides of the main channel. The main channel contains four contraction and expansion structures, and one electrode with a thickness of 150um is arranged on both sides of the main channel. The length of the main channel is 6 mm, the height is 150 um, the width of the constricted part of the channel is 200 um and the width of the expanded part is 600 um, and the shape of the expanded part on one side of the channel is a semicircle with a radius of 200 um.

[0047] The microfluidic cell sorting chip of this example was prepared by the following method:

[0048] Using conductive carbon ink as the material, microelectrodes (electrode thickness 150um) were prepared on the float glass substrate material by screen printing; using polydimethylsiloxane (PDMS) as the material, micro-ma...

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Abstract

The invention belongs to the technical field of cell sorting, and discloses a micro-fluidic cell sorting chip and a micro-fluidic cell sorting method coupled with dielectrophoresis and spatial isolation. The micro-fluidic cell sorting chip comprises a main channel and electrodes arranged on two sides of the main channel, wherein a plurality of horizontal contraction and expansion structures are arranged in the main channel; an alternating electric field is applied to the electrodes of the sorting chip, different cells generate different dielectrophoresis forces in the alternating electric field due to the differences of the intrinsic dielectric properties, and thus deviation of different degrees is generated in the main channel; at the same time, the flowing cells suffer from the horizontal transfer force generated by the contraction and expansion structures of the main channel, due to the coupling of the two effects, different cells flow out from different outlets, and thus the sorting of the cells is realized. With the chip and the method provided by the invention, the difficulty that the dielectric differences of different cells are not obvious existing in the traditional cell sorting only adopting dielectrophoresis is solved, and the defect that a channel of the chip adopted for cell sorting only adopting the spatial isolation technology is too long is also overcome.

Description

technical field [0001] The invention belongs to the technical field of cell sorting, and in particular relates to a microfluidic cell sorting chip and method coupled with dielectrophoresis and space separation. Background technique [0002] Currently commercialized cell sorting products are mainly divided into two categories. The first category is flow cytometry. Fluorescently labeled cell suspension is wrapped and pushed by the sheath fluid, and the sprayed mixed solution is broken into uniform droplets by the ultra-high frequency piezoelectric crystal. Under the irradiation of the laser beam, according to the scattered light and fluorescence of the cells Intensity achieves sorting. The disadvantage of this method is that it is prone to cell contamination and the equipment is huge. The second type is immunomagnetic bead sorting. The magnetic beads are combined with the cells through specific antibodies, and the magnetic beads carry the bound cells or are adsorbed on the ...

Claims

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Application Information

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IPC IPC(8): C12M1/34C12M1/00
CPCC12M23/16C12M47/04
Inventor 董华姚洁陈景煊曹晓东
Owner SOUTH CHINA UNIV OF TECH
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