Unlock instant, AI-driven research and patent intelligence for your innovation.

DNA nano-imaging probe for subcellular-scale cellular co-localization as well as preparation method and application of DNA nano-imaging probe

An imaging probe and subcellular technology, applied in the field of DNA imaging probes, can solve problems such as unclear mechanism, poor histocompatibility, difficult metabolism, etc., and achieve enhanced penetration and targeting, good biocompatibility, The effect of avoided damage

Inactive Publication Date: 2018-05-04
SHANGHAI NAT ENG RES CENT FORNANOTECH
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Such probes are usually composed of inorganic substances or metal substances, which have certain biological toxicity, poor tissue compatibility, difficult metabolism and unknown mechanism

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • DNA nano-imaging probe for subcellular-scale cellular co-localization as well as preparation method and application of DNA nano-imaging probe
  • DNA nano-imaging probe for subcellular-scale cellular co-localization as well as preparation method and application of DNA nano-imaging probe

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Dissolve the replacement strand DNA 1 with fluorescent Cy5 and the 207 staple strands of DNA2-208 in MilliQ water to make the final concentration of each strand 200nM; mix M13mp18 single-stranded DNA (100nM) with The 208 short chains (200nM) were mixed in 1×TAE-Mg with a molar concentration ratio of 1:5 2+ In the solution, the final concentration of M13mp18 single-stranded DNA is 5nM, and the final concentration of short-chain is 50nM. Put the mixed solution into the PCR instrument, set the reaction level at 95°C for 3 minutes, then slowly cool down to 4°C, and the cooling rate is 0.2°C / 10s. A DNA nano-imaging probe is obtained.

[0026] Add the DNA nano-imaging probes prepared in the above steps into the cell culture medium and co-incubate with the cells for 12 hours. After the cultivation, the culture solution containing the DNA nano-imaging probe was discarded, and the residual culture solution was washed away with PBS. Then the cells were fixed with paraformaldeh...

Embodiment 2

[0028] The replacement strand DNA 1 with fluorescent Cy3 and 207 staple strands of other sequence numbers were dissolved in MilliQ water in equal amounts so that the final concentration of each strand was 200 nM. Mix the M13mp18 single-stranded DNA (100nM) with the mixed 208 short strands (200nM) at a molar concentration ratio of 1:10 in 1×TAE-Mg 2+ In the solution, the final concentration of M13mp18 single-stranded DNA is 5nM, and the final concentration of short-chain is 50nM. Put the mixed solution into the PCR instrument, set the reaction level at 95°C for 3 minutes, then slowly cool down to 4°C at a cooling rate of 0.2°C / 10s to obtain DNA nano-imaging probes.

[0029] Add the DNA nano-imaging probes prepared in the above steps into the cell culture medium and co-incubate with the cells for 10 hours. After the cultivation, the culture solution containing the DNA nano-imaging probe was discarded, and the residual culture solution was washed away with PBS. Then the cells w...

Embodiment 3

[0031] Dissolve the replacement strand DNA 1 with fluorescent FITC in equal amounts with 207 staple strands of other sequence numbers into MilliQ water so that the final concentration of each strand is 200 nM. Mix the M13mp18 single-stranded DNA (100nM) with the mixed 208 short strands (200nM) at a molar concentration ratio of 1:7 in 1×TAE-Mg 2+ In the solution, the final concentration of M13mp18 single-stranded DNA was 5 nM, and the final concentration of short-stranded DNA was 50 nM. Put the mixed solution into the PCR instrument, set the reaction level at 95°C for 3 minutes, then slowly cool down to 4°C at a cooling rate of 0.2°C / 10s to obtain DNA nano-imaging probes.

[0032]Add the DNA nano-imaging probes prepared in the above steps into the cell culture medium and co-incubate with the cells for 2 hours. After the cultivation, the culture solution containing the DNA nano-imaging probe was discarded, and the residual culture solution was washed away with PBS. Then the ce...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a DNA nano-imaging probe for subcellular-scale cellular co-localization as well as a preparation method and application of the DNA nano-imaging probe. The preparation method comprises the following steps: equally dissolving fluorescent substitution chain DNA 1 and 207 staple chains into MilliQ water until the final concentration of each chain is 200nM, and mixing M13mp18 single stranded DNA (100nM) with 208 short chains (200mM) in a 1*TAE-Mg<2+> solution in a molar concentration ratio of (1 to 5)-(1 to 10), wherein the final concentration of M13mp18 single stranded DNAis 5nM, and the final concentration of the short chains is 50nM; and introducing the mixed solution into an PCR instrument, setting the reaction temperature at 95 DEG C, standing for 3 minutes, and slowly cooling to 4 DEG C at a cooling speed of 0.2 DEG C / 10s. By virtue of the small size and the relatively good biocompatibility of a DNA origami structure, the DNA nano-imaging probe has relativelylarge application prospects in the fields of subcellular-scale imaging, tumor detection and drug mechanism research.

Description

technical field [0001] The invention proposes a preparation method of a DNA nano-imaging probe for subcellular scale cell co-localization, as well as its product and application. Specifically, the DNA single strand marked with a specific fluorescent molecule is modified on the DNA triangular origami structure through self-assembly, and becomes a DNA imaging probe, so as to realize the function of organelle colocalization. Due to the small size and better biocompatibility of the DNA origami structure, the invention has great application prospects in the fields of subcellular scale imaging, tumor detection and drug mechanism research. Background technique [0002] At present, there are mainly the following types of nanoprobes for subcellular scale imaging and organelle colocalization: nanoprobes based on magnetic materials, such nanoprobes are mainly based on iron or iron compounds; nanoprobes based on gold nanoparticles and Detection probes for their complexes; and nanoprobe...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/6806G01N33/569
CPCC12Q1/6806G01N33/56966C12Q2531/113
Inventor 何丹农王萍陈益刘婷徐艳金彩虹
Owner SHANGHAI NAT ENG RES CENT FORNANOTECH
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com