Mycobacterium tuberculosis h37rv coding gene and its application

Abstract

The invention relates to a mycobacterium tuberculosis H37Rv coding gene, which can be used as a standard gene for molecular identification of mycobacterium tuberculosis complex, and is used for molecular identification and clinical detection of mycobacterium tuberculosis complex.

Description

technical field [0001] The invention relates to the field of gene detection, in particular to the identification of pathogenic bacteria species. Background technique [0002] Mycobacterium tuberculosis (MTB) is the causative bacterium of human tuberculosis. It can invade all organs of the body, but tuberculosis is the most common. Tuberculosis is an extremely important infectious disease, which seriously threatens human life and health. About 8 million new cases occur each year, and at least 3 million people die from the disease, according to WHO. The clinical strains of MTB are difficult to cultivate, grow slowly, can cross-infect with other mycobacteria, and are indistinguishable from tuberculosis and other respiratory tract infections, which bring great difficulties to rapid clinical diagnosis and treatment. Therefore, the establishment of a rapid, accurate, specific, sensitive and inexpensive detection method for tuberculosis is a necessary prerequisite for effective ...

AI Technical Summary

Problems solved by technology

Although the three types of methods have their own advantages, they also have disadvantages, such as the long period of traditional isolation and culture and the low culturability of bacteria; the current molecular level detection is still poor in specificity, sensitivity and simplicity; Composition characteristic analysis is costly and complicated to operate

However, since MTB belongs to prokaryotes, due to the deficiencies of prokaryotic genome annotation technology itself, there may still be annotation errors in genome annotation (over-annotation, gene boundary errors, and ORF start and end site errors, alternative splicing, ribosome displacement, missing annotations), which has brought troubles to the in-depth and accurate analysis of biological mechanisms

To solve this problem, Proteogenomics has been used to correct the annotated genes of H37Rv, however, the high rate of false positives, conventional techniques are difficult to predict annotation genes, new gene verification, new gene function analysis and its application, etc. are the challenges faced by the field

[0005] In general, the traditional identification strategy of Mycobacterium tuberculosis complex (MTBC) has the defects of long period, cumbersome steps, low specificity and sensitivity, etc.

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