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ALDH2 gene detection kit and ALDH2 gene detection method
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A gene detection and kit technology, applied in the fields of genetic engineering and molecular biology, can solve the problems of low sensitivity, high false positive rate, and small throughput of ALDH2 gene detection, and achieve short detection period, reduce false positive rate, and pass the test. high volume effect
Inactive Publication Date: 2018-05-11
GUANGZHOU KANGXINRUI GENE HEALTH TECH CO LTD
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[0007] The purpose of the present invention is to provide an ALDH2 gene detection kit and detection method, aiming to solve the technical problems of low ALDH2 gene detection sensitivity, high false positive rate, long cycle and low throughput in the prior art
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no. 1 example
[0026] The present invention proposes a first embodiment, an ALDH2 gene detection kit, comprising a primer set for specific amplification of nucleic acid fragments containing the rs671 site, said primer set including upstream primers SEQ ID NO: 1, SEQ ID NO: 1, SEQ ID NO: ID NO:2, SEQ ID NO:3 or SEQ ID NO:4, and downstream primers SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7 or SEQ ID NO:8.
[0027] The ALDH2 gene detection kit provided by this program includes a specific amplification primer set, which can specifically amplify nucleic acid fragments containing the rs671 site, reducing the possibility of amplifying non-target fragments, thereby The detection sensitivity of the rs671 site is high, and the false positive rate is low.
[0028] Preferably, the ALDH2 gene detection kit also includes a linker, and the linker includes at least one of linker A, linker B, linker C, and linker D; the linker A consists of SEQ ID NO:9 and SEQ ID NO: 10; the joint B consists of SEQ ID NO: 11 and...
no. 2 example
[0033] The present invention proposes a second embodiment, a gene detection method, comprising the following steps:
[0034] A. Utilize the amplification primer set to amplify the sample containing the rs671 site to obtain an amplified product; it is characterized in that the primer set includes upstream primers SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO : 3 or SEQ ID NO: 4, and downstream primers SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7 or SEQ ID NO: 8;
[0035] B. Connecting adapters to the amplification products to obtain library molecules;
[0036] C. Using the second generation high-throughput gene sequencing technology to sequence the library molecules, and determine the genotype of the rs671 site.
[0037]The gene detection method provided by the invention uses a specific amplification primer set to amplify the nucleic acid fragment containing the rs671 site, thereby improving the detection sensitivity of the rs671 site and reducing the false positive rate of detection.
[0...
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Abstract
The invention relates to the fields of genetic engineering and molecular biology, and discloses an ALDH2 gene detection kit. The detection kit comprises a primer group for specifically amplifying a nucleic acid fragment containing an rs671 site, the primer group comprises an upstream primer represented by SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3 or SEQ ID NO:4, and a downstream primer represented bySEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7 or SEQ ID NO:8. The invention also provides a gene detection method. The kit uses the specific amplification primer group to amplify the nucleic acid fragment containing the rs671 site, so the detection sensitivity of ALDH2 genemutation is high, and the false positive rate is low; and the kit adopts a second generation high-throughput gene sequencing technology to detect, so short detection period and high throughput are achieved.
Description
technical field [0001] The invention relates to the fields of genetic engineering and molecular biology, and more specifically relates to an ALDH2 gene detection kit and detection method. Background technique [0002] Human acetaldehydedehydrogenase (ALDH) is a tetrameric protein that catalyzes the oxidation of acetaldehyde and other aliphatic aldehydes. At present, it has been found that there are 19 isoenzymes of ALDH, mainly including ALDH1-4, among which ALDH2 is the most important. [0003] Nitroglycerin is a classic drug for treating angina pectoris. Studies have found that the vasodilation effect of triglycerin is mediated by the release of nitric oxide (NO). However, sublingual administration of nitroglycerin in some patients clinically cannot relieve angina pectoris quickly and effectively, and aggravates severe myocardial ischemia. It was recently found that the ALDH2 gene is closely related to the conversion of nitroglycerin to NO. In addition, ALDH2 is highly...
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