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Preparation method and use method of pichia kudriavzevii adsorbent capable of quickly adsorbing heavy metal

A yeast adsorbent and a technology for adsorbing heavy metals are applied in the field of preparation of Pichia kudzu adsorbents, and can solve the problems of high cost, generation of secondary organisms, secondary pollution and the like

Inactive Publication Date: 2018-05-15
合肥郑国生物科技有限公司 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] In the traditional heavy metal adsorption technology, a large number of chemical reagents are used for precipitation and reduction, but further secondary organisms are generated, resulting in secondary pollution
At present, many reports on new adsorbent adsorption methods for heavy metals such as mercury, cadmium, lead, chromium, and arsenic have been published, such as tannin gels modified by amines, chelating polymers and resins, etc., but these methods are costly , time-consuming

Method used

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  • Preparation method and use method of pichia kudriavzevii adsorbent capable of quickly adsorbing heavy metal
  • Preparation method and use method of pichia kudriavzevii adsorbent capable of quickly adsorbing heavy metal
  • Preparation method and use method of pichia kudriavzevii adsorbent capable of quickly adsorbing heavy metal

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preparation example Construction

[0034] 2. Preparation of Pichia kurdori adsorbent:

[0035] (1) Activation culture of Pichia kurdori:

[0036] Inoculate the Pichia kurdori seed solution into the fermentation medium with a 10% (v / v) inoculum volume, the liquid volume is 45mL / 250mL, the shaker speed is 250r / min, and the temperature is cultivated at 25°C for 16h;

[0037] (2) Enrichment culture of Pichia kurdori:

[0038] Add 3.5L fermentation broth to a 7L fermenter, the inoculum size is 12% (v / v), the initial stirring speed is 400r / min, the initial ventilation volume is 1.0 vvm, the fermentation temperature is 24.5-25.5°C, and 3mol / L NaOH is used in the fermentation process Adjust the pH of the solution to maintain it at 4.0±0.1, maintain the dissolved oxygen above 30% during the fermentation process, set the maximum stirring speed to 800r / min, and the maximum ventilation rate to 3.0 vvm, start feeding at the 6th hour of fermentation, and feed once every 2h to make up When the sugar concentration reaches 2%...

Embodiment 1

[0047] Use 0.1mol / L HCl solution to heat and boil Pichia pastoris for 15 minutes, then centrifuge at 1000-3000rpm to collect the bacteria, put it into an oven for drying at 105°C, and grind it into powder for later use.

[0048] Use 0.1mol / L NaOH solution to heat and boil Pichia pastoris for 15 minutes, then centrifuge at 3000-5000rpm to collect the bacteria, put them in an oven at 105°C, dry them, and grind them into powder for later use.

[0049] Prepare 100mL 100mg / L heavy metal mercury, chromium, lead, chromium and arsenic solution in a conical flask, take 0.1g of the above-mentioned bacterial adsorbent powder and add them to the conical flask respectively, stop the bottle mouth with a cotton plug, and put it at 30 ℃, 200rpm treatment for 60min, calculate the adsorption rate, the result is as follows figure 1 shown.

[0050] It can be seen from the figure that the adsorption capacity of heavy metal mercury, chromium, lead, chromium and arsenic by HCl-treated Pichia kurdor...

Embodiment 2

[0052] Heat and boil Pichia pastoris with 0.1mol / L NaOH solution for 15 minutes, centrifuge at 1000-3000rpm, collect the bacteria, put them into an oven for drying at 105°C, and grind them into powder.

[0053] Prepare 100mL 100mg / L heavy metal mercury, chromium, lead, chromium and arsenic solution in a conical flask, take 0.1g of the above-mentioned bacterial adsorbent powder and add them to the conical flask respectively, stop the bottle mouth with a cotton plug, and put it at 30 ℃, 200rpm respectively treated for 5min, 10min, 20min, 30min, 60min, 90min, 120min, 150min, 180min, 240min, 300min, 360min to calculate the adsorption rate, the results are as follows figure 2 shown.

[0054] It can be seen from the figure that the adsorption of heavy metal mercury, lead and arsenic by NaOH-treated Pichia kurdori was the highest at 30 min, which were 68 mg / g, 65 mg / g and 70 mg / g respectively; the adsorption of heavy metals cadmium and The adsorption amount of chromium was the high...

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Abstract

The invention discloses a preparation method of a pichia kudriavzevii adsorbent capable of quickly adsorbing heavy metal. In the preparation method disclosed by the invention, a high-concentration pichia kudriavzevii solution can be obtained by a supplementary material batch culture mode, and the pichia kudriavzevii adsorbent can be prepared by mixing and boiling the pichia kudriavzevii solution with a NaOH or HCl inorganic ligand solution. The pichia kudriavzevii adsorbent can be applied to adsorbing heavy metal of mercury, cadmium, lead, chromium, arsenic and the like in wastewater. The invention further discloses a use method of the adsorbent. Adsorption characteristics of the pichia kudriavzevii adsorbent are researched from four aspects of inorganic ligand pretreatment, adsorption time, adsorption thallus concentration and adsorption temperature, and heavy metal ion adsorbing modes and the best adsorption environment of the pichia kudriavzevii are explored.

Description

technical field [0001] The invention belongs to the field of sewage treatment, and in particular relates to a preparation method and a use method of a Pichia kurdori adsorbent that rapidly adsorbs heavy metals. Background technique [0002] In the traditional heavy metal adsorption technology, a large number of chemical reagents are used for precipitation and reduction, but further secondary organisms are generated, resulting in secondary pollution. At present, many reports have been published on new adsorbent adsorption methods for heavy metals such as mercury, cadmium, lead, chromium, arsenic, etc., such as tannin gels modified by amines, chelating polymers and resins, etc., but these methods are costly , Time-consuming. Therefore, it is necessary to find an economical and environmentally friendly heavy metal adsorbent and preparation method. [0003] Microbial adsorption method is a new technology developed in recent years, which has the advantages of abundant strains...

Claims

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Application Information

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IPC IPC(8): B01J20/24B01J20/30C02F1/28C02F101/20C02F101/22
CPCB01J20/24C02F1/286C02F2101/20C02F2101/22
Inventor 詹晓北李志涛高敏杰朱莉李国
Owner 合肥郑国生物科技有限公司
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