Low-pesticide residue ginseng stem leaf extract and preparation method thereof
A technology of ginseng stems and leaves and extracts, which is applied in the direction of pharmaceutical formulas, plant raw materials, medical preparations containing active ingredients, etc., can solve problems such as low yield, waste of reagents, and environmental pollution, and achieve easy operation and high production technology. easy effect
Active Publication Date: 2018-05-18
杨凌萃健生物工程技术有限公司
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Abstract
The invention discloses a low-pesticide residue ginseng stem leaf extract and a preparation method thereof. The method comprises the following steps that 1, ethanol extraction: ginseng stem leaves aretaken to be pulverized into coarse powder, ethanol is added and heated, refluxing extraction is conducted twice, filtering is conducted, filtrates are combined, decompression concentration is conducted, and water is added for concentration until smell of the ethanol is completely removed to obtain concentrated liquid; 2 impurity removal: impurity removal is conducted on the concentrated liquid obtained in step 1, centrifugal filtration is conducted, and supernatant liquid is obtained; 3, purification: decompression concentration is conducted on the supernatant liquid obtained in step 2, concentration is conducted to obtain extract, methanol solution is added, filtering is conducted by using filter paper, the filtrate is subjected to gel column chromatography to collect lower column liquids, thin-layer chromatography is adopted for detection, the lower column liquids with Rg1, Re and Rd spots are combined, and decompression concentration, drying and pulverizing are conducted to obtainthe low-pesticide residue ginseng stem leaf extract. According to the extract, ginsenoside content in a ginseng stem leaf extract prepared through the method is high, pesticide residue is low, whereinthe ginsenoside content (Rg1, Re and Rd) is larger than or equal to 65%, and the pesticide residue indexes conform to an EC396 standard.
Application Domain
Plant ingredients
Technology Topic
Thin-layer chromatographyChemistry +11
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Property | Measurement | Unit |
Mesh | 20.0 ~ 30.0 | mesh |
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