Low-pesticide residue ginseng stem leaf extract and preparation method thereof

Abstract

The invention discloses a low-pesticide residue ginseng stem leaf extract and a preparation method thereof. The method comprises the following steps that 1, ethanol extraction: ginseng stem leaves aretaken to be pulverized into coarse powder, ethanol is added and heated, refluxing extraction is conducted twice, filtering is conducted, filtrates are combined, decompression concentration is conducted, and water is added for concentration until smell of the ethanol is completely removed to obtain concentrated liquid; 2 impurity removal: impurity removal is conducted on the concentrated liquid obtained in step 1, centrifugal filtration is conducted, and supernatant liquid is obtained; 3, purification: decompression concentration is conducted on the supernatant liquid obtained in step 2, concentration is conducted to obtain extract, methanol solution is added, filtering is conducted by using filter paper, the filtrate is subjected to gel column chromatography to collect lower column liquids, thin-layer chromatography is adopted for detection, the lower column liquids with Rg1, Re and Rd spots are combined, and decompression concentration, drying and pulverizing are conducted to obtainthe low-pesticide residue ginseng stem leaf extract. According to the extract, ginsenoside content in a ginseng stem leaf extract prepared through the method is high, pesticide residue is low, whereinthe ginsenoside content (Rg1, Re and Rd) is larger than or equal to 65%, and the pesticide residue indexes conform to an EC396 standard.

Description

technical field [0001] The invention belongs to the field of plant extraction, and in particular relates to a low pesticide residue ginseng stem and leaf extract and a preparation method thereof. Background technique [0002] Ginseng is a plant of the genus Panax of Araliaceae. It is a traditional Chinese medicinal material. Its name was first recorded in "Shen Nong's Materia Medica". Evil, eyesight, happy puzzle, long-term service light body and prolong life." [0003] Ginseng contains a variety of ginsenosides, volatile oils, amino acids, trace elements, organic acids, sugars, cellulose and other components, among which ginsenoside (GS) is the main active ingredient of ginseng and the main index for evaluating ginseng. With the development of modern medicine and pharmacology, scholars at home and abroad have conducted in-depth research on the pharmacological effects of ginsenosides. Studies have found that ginsenosides have anti-fatigue, anti-stress, anti-depression, memo...

AI Technical Summary

Problems solved by technology

[0016] The existing patent application number CN104490966A discloses a method for removing residual pesticides in ginseng stem and leaf extracts; the method extracts ginseng stem and leaf total saponins aqueous solution by mixing organic solvents, and then dilutes the mother liquor through macroporous resin, 65-80 % elution, collect the eluate, and dry to obtain the finished product; but this process does not mention obstinate pesticides, such as propamocarb, oxaxyl, etc., and the mixed solvent used is difficult to recycle and reuse, resulting in waste and pollution of reagents. Increase cost, not suitable for mass production

[0017] It can be seen that there are many defects in the prior art in the process of extracting ginseng stem and leaf extracts with low pesticide residues, especially in the removal of stubborn pesticide residues and industrial production. residual, cumbersome operation, serious environmental pollution, and low yield; this shows that the existing technical research is still in its infancy and cannot really reach the level of industrialized large-scale production and market promotion.

Images