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A kind of method for synthesizing benastatin G

A benastatin and synthetic route technology, applied in the field of medicinal chemical synthesis, can solve the problems of difficulty in chemically synthesizing benstatin G, no chemical synthesis of benstatin G, complex chemical structure, etc., and achieves strong practical value and low production cost. , the effect of simple operation

Active Publication Date: 2021-04-20
EAST CHINA NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] As a new type of natural statin drug, benastatin G has broad application prospects, but due to the complex chemical structure of benastatin G, it is difficult to chemically synthesize benastatin G, and the currently obtained benastatin G is usually obtained from chain It is isolated from the genus Mycobacterium, and there is no report on the chemical synthesis of benastatin G

Method used

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  • A kind of method for synthesizing benastatin G
  • A kind of method for synthesizing benastatin G
  • A kind of method for synthesizing benastatin G

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0116] Embodiment 1: Compound shown in preparation formula 3:

[0117] Step a: prepare the compound shown in formula 8:

[0118]

[0119] The compound of formula 7 (3.51g, 19.49mmol) was dissolved in N,N-dimethylformamide (20mL), and then phosphorus oxychloride (2.68mL, 29.23mmol) was slowly added dropwise at 0°C under nitrogen protection, After the dropwise addition, stir the reaction at 90°C for 3 hours to end the reaction. After the reaction solution is cooled to room temperature, it is slowly poured into ice water. The resulting mixed solution is adjusted to pH 10 with 20 wt% sodium hydroxide solution, and then extracted with ether. , the organic phases were combined, and the organic phase obtained was washed once with saturated sodium bicarbonate solution and saturated brine successively, dried with anhydrous sodium sulfate, filtered, and the filtrate was concentrated under reduced pressure until no solution was distilled off, and the residue was analyzed by column chr...

Embodiment 2

[0147] Embodiment 2 prepares the compound shown in formula 4:

[0148] Step A: prepare the compound shown in formula 14:

[0149]

[0150] The compound of formula 13 (8.08g, 25.58mmol) was dissolved in N,N-dimethylformamide (64mL), and potassium carbonate (7.78g, 56.27mmol) and dimethyl sulfate (5.09mL, 53.72mmol) were added, Then react at 60°C for 21.5 hours to end the reaction, cool the reaction liquid to room temperature, quench with saturated ammonium chloride solution, extract with ethyl acetate, combine the organic phases, and wash the organic phases twice with water, saturated chlorinated Sodium was washed once, dried with anhydrous sodium sulfate, filtered, concentrated, and the residue was separated by column chromatography (5% ethyl acetate / petroleum ether) to obtain the compound of formula 14 (7.74g, yield rate 88%), Rf= 0.56 (20% ethyl acetate / petroleum ether).

[0151] Tested: 1 H NMR (300MHz, CDCl 3 )δ6.46(s,2H),3.91(s,3H),3.83(s,6H)ppm;

[0152] 13 C NM...

Embodiment 3

[0196] Embodiment 3 prepares the compound shown in formula 1:

[0197] Step ①: prepare the compound shown in formula 5:

[0198]

[0199] The compound of formula 3 (907mg, 2.07mmol) was dissolved in a mixed solvent of N,N-dimethylformamide (5mL) and diisopropylamine (3mL), and tetrakistriphenylphosphine palladium (239.2mg, 0.207mmol) was added , cuprous iodide (19.7mg, 0.104mmol), after freezing and deoxygenating with liquid nitrogen, add 2.5mL of N, N-dimethylformamide solution of formula 4 compound (496.2mg, 2.17mmol) of freezing and deoxygenating, and then React at 45°C for 3 hours to end the reaction. After cooling the reaction liquid to room temperature, add saturated ammonium chloride to quench, extract with ethyl acetate, combine the organic phases, wash the obtained organic phases twice with distilled water, and then with saturated sodium chloride. Once, dried over anhydrous sodium sulfate, filtered, concentrated, and the residue was separated by column chromatogra...

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Abstract

The invention discloses a method for synthesizing benastatin G, which comprises the following steps (2) or steps (1) to (2) in the synthesis route: wherein: R 1 , R 2 , R 3 each independently selected from C 1 ~C 8 alkyl. The present invention realizes the chemical synthesis of benastatin G for the first time, and has extremely strong practical value for realizing the industrialized preparation of benastatin G, and the preparation method of the present invention has the advantages of simple operation, safety and pollution-free, no special requirements for equipment, and production The advantages of low cost are of great significance to the realization of large-scale production.

Description

technical field [0001] The invention relates to a method for synthesizing benastatin G, which belongs to the technical field of pharmaceutical chemical synthesis. Background technique [0002] Benastatin (Benastatin) is a new type of natural drug, the more mature benastatin compounds are Benastatin A (Benastatin A, CAS#138968-85-1), Benastatin B (Benastatin B , CAS#138968-86-2), Benastatin C (Benastatin C, CAS#150151-88-5), Benastatin D (Benastatin D, CAS#150151-89-6), etc. [0003] Christian Hertweck et al. isolated a new benastatin compound: benastatin G, and found that benastatin G exhibited good biological activity against human cervical cancer cells, with an IC50 of 9.4 μg / mL. The structural formula of benastatin G is as follows: [0004] [0005] As a new type of natural statin drug, benastatin G has broad application prospects, but due to the complex chemical structure of benastatin G, it is difficult to chemically synthesize benastatin G, and the currently obtai...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D311/78
CPCC07D311/78
Inventor 辛坤云陈彦宇杨鲍潮高栓虎
Owner EAST CHINA NORMAL UNIV
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