Unlock instant, AI-driven research and patent intelligence for your innovation.

Electrochemical sensor for detecting acetamiprid, preparation method and applications thereof

An acetamiprid and sensor technology, which is applied in the field of electrochemical sensors for detecting acetamiprid and the field of preparation thereof, can solve the problems of small molecular weight, inability to detect, not enough to bind two antibodies at the same time, etc., and achieves easy portability, convenient electrodes, Accurate and reliable test results

Inactive Publication Date: 2018-05-18
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
View PDF3 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These substances to be detected usually contain multiple recognition sites and can be detected by conventional sandwich immunoassays, but pesticides such as acetamiprid cannot be detected by sandwich immunoassays due to their small molecular weights and insufficient binding to two antibodies at the same time

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Electrochemical sensor for detecting acetamiprid, preparation method and applications thereof
  • Electrochemical sensor for detecting acetamiprid, preparation method and applications thereof
  • Electrochemical sensor for detecting acetamiprid, preparation method and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0044] The present invention also provides a preparation method for the detection electrode, which includes the following steps: 1) soaking the cleaned gold electrode in an ethanol solution of zinc dihydrogensulfate to carry out self-assembly of Au-S bonds, forming Zinc dihydrogen sulfate self-assembly layer; 2) the surface of the zinc dihydrogen sulfate self-assembly layer obtained in the step 1) is coated with MES buffer containing NHS and EDC, and the zinc dihydrogen sulfate self-assembly layer is modified Activation, forming a dihydrolipoic acid activation layer on the surface of the gold electrode; 3) coating the acetamiprid capture antibody solution on the surface of the zinc dihydrogensulfate activation layer for incubation, forming acetamiprid on the outer surface of the DHLA activation layer amidine antibody layer; 4) coating the outer surface of the acetamiprid antibody layer with MCH solution for sealing, forming an MCH sealing layer on the outer surface of the aceta...

Embodiment 1

[0086] A kind of preparation and verification of the sandwich type electrochemical sensor of the present invention, comprises the following steps:

[0087] a. The gold electrode is first polished in 0.3 and 0.05 μm alumina slurry until it becomes a mirror surface, and then rinsed with secondary water;

[0088] b. Soak the gold electrode in H 2 SO 4 (98%) and H 2 o 2 (30%) in a mixed solution (volume ratio) with a ratio of 3:1, after 20 min, ultrasonically wash several times with secondary water, then wash with 0.25mM KCl and 5.0mM K 3 [Fe(CN) 6 ] of 10mM PBS as the bottom solution, using EIS and CV technology to read K 3 [Fe(CN) 6 ] signal changes;

[0089] c. Configure 1mM DHLA ethanol solution, soak the pretreated gold electrode in the ethanol solution of DHLA for two hours, and DHLA self-assembles on the surface of the gold electrode through the Au-S bond;

[0090] d. Configure 0.1M MES buffer solution (pH=6.5) containing 5mM NHS and 2.5mM EDC, take 8uL to modify th...

Embodiment 2

[0097] A kind of control experiment verification of the sandwich type electrochemical sensor of the present invention comprises the following steps:

[0098] a, 6 gold electrodes (named a, b, c, d, e, f) are first polished in 0.3 and 0.05 μm alumina slurry until they are mirror-like, and rinsed with secondary water;

[0099] b. Soak the gold electrode in H 2 SO 4 (98%) and H2 o 2 (30%) in the mixed solution (volume ratio) that the ratio is 3:1, after 20min, use secondary water to ultrasonically wash several times, blow dry with nitrogen for subsequent use;

[0100] c. Configure 1mM DHLA ethanol solution, soak the pretreated gold electrode in the ethanol solution of DHLA for two hours, and DHLA self-assembles on the surface of the gold electrode through the Au-S bond;

[0101] d. Configure 0.1M MES buffer solution (pH=6.5) containing 5mM NHS and 2.5mM EDC, take 8uL to modify the carboxyl group on the surface of the electrode to activate the surface of DHLA;

[0102] d. Diss...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Concentrationaaaaaaaaaa
Volumeaaaaaaaaaa
Login to View More

Abstract

The invention provides an electrochemical sensor for detecting acetamiprid, and a preparation method thereof. The electrochemical sensor comprises a detection electrode and a matching reagent, whereinthe detection electrode comprises a gold electrode, a DHLA activation layer, an acetamiprid antibody layer and an MCH blocking layer, the exterior of the gold electrode is sequentially wrapped with the DHLA activation layer, the acetamiprid antibody layer and the MCH blocking layer, and the matching reagent comprises an acetamiprid hapten probe solution and a methylene blue probe solution. According to the present invention, the electrochemical sensor achieves the immunodetection of small molecules, the detection is rapid, the detection limit is 3.2 ng / L, the detection is sensitive, and the use method is simple and rapid, and it is suitable for the online detection of acetamiprid in food safety.

Description

technical field [0001] The invention relates to the technical field of electrochemical sensors, in particular to an electrochemical sensor for detecting acetamiprid and a preparation method thereof. Background technique [0002] The new insecticide acetamiprid belongs to nitromethylene heterocyclic compounds, which can act on the nicotinic acetylcholine receptors in the synaptic parts of the insect nervous system, interfere with the stimulation conduction of the insect nervous system, cause the blockage of the nervous system pathway, and cause neuropathy. Accumulation of the transmitter acetylcholine at synaptic sites leads to paralysis and eventual death of the insect. Acetamiprid has replaced conventional insecticides such as organophosphates and carbamates and is widely used around the world. Acetamiprid is similar to many other pesticides, and there are inevitably residues in the application process, and the food safety problems caused by pesticide residues have become ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N27/327G01N27/30
CPCG01N27/307G01N27/3276G01N27/3277
Inventor 徐霞红王新全王祥云齐沛沛汪志威徐浩王强
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES