Polypeptide composition for inhibiting melanogenesis and application thereof

A polypeptide composition and melanin production technology, applied in the directions of drug combinations, dipeptide components, tripeptide components, etc., can solve problems such as single action mechanism, achieve stable polypeptide structure, good whitening effect and safety, and not easy to lose. live effect

Active Publication Date: 2018-05-22
ZHUHAI UNITED LAB
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The mechanism of action of the three peptides in the whitening agent component to inhibit melanin production is reflected in tyrosinase, the mechanism of action is relatively simple, and there may be a risk of irreversible depigmentation and cytotoxicity

Method used

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  • Polypeptide composition for inhibiting melanogenesis and application thereof
  • Polypeptide composition for inhibiting melanogenesis and application thereof
  • Polypeptide composition for inhibiting melanogenesis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1 9

[0054] Example 1 Effect experiment of nonapeptide-1 on melanogenesis

[0055] The nonapeptide-1 test was divided into four groups: PBS blank control group, nonapeptide-1 0.01μg / ml group, nonapeptide-1 0.05μg / ml group, nonapeptide-1 0.1μg / ml group.

[0056] MTT detection of cell proliferation rate: the mouse melanoma cell B16 was mixed with 4×10 4 cells / well were seeded in a 96-well plate, and after the cells adhered to the wall, the medium was replaced with fresh culture medium containing different concentrations of nonapeptide-1 (0, 0.01, 0.05 and 0.1 μg / ml respectively) (i.e. DMEM-high glucose culture) Base) 1 time, and 3 replicate wells were set up for each group. 37°C, 5% CO 2 After culturing in the incubator for 24 hours, carefully discard the culture medium, add 100 μl of MTT solution (5 mg / ml) to each well, continue to cultivate for 4 hours, carefully discard the supernatant, add 150 μl of DMSO to each well, and shake for 15 minutes to make the precipitation fully Di...

Embodiment 2

[0060] Example 2 Effect experiment of polypeptide combination on melanogenesis

[0061] The experiment was divided into 4 groups with a total of 23 groups, including the following groups:

[0062] (1) Control group

[0063] Blank PBS group, nonapeptide-1 0.01μg / ml group, nonapeptide-1 0.05μg / ml group, nonapeptide-1 0.1μg / ml group, carnosine 8000μg / ml group, palmitoyl tetrapeptide-7 0.7μg / ml group Group.

[0064] (2) Carnosine and nonapeptide-1 composition experiment group

[0065] Carnosine 6000μg / ml+nonapeptide-1 0.01μg / ml group, carnosine 8000μg / ml+nonapeptide-1 0.01μg / ml group, carnosine 10000μg / ml+nonapeptide-1 0.01μg / ml group;

[0066] Carnosine 6000μg / ml+nonapeptide-1 0.05μg / ml group, carnosine 8000μg / ml+nonapeptide-1 0.05μg / ml group, carnosine 10000μg / ml+nonapeptide-1 0.05μg / ml group;

[0067] Carnosine 6000 μg / ml + nonapeptide-1 0.1 μg / ml group, carnosine 8000 μg / ml + nonapeptide-1 0.1 μg / ml group, carnosine 10000 μg / ml + nonapeptide-1 0.1 μg / ml group.

[0068] (3...

Embodiment 3

[0077] Example 3 Polypeptide Effect Experiment on Tyrosinase Activity

[0078] The experiment was divided into 2 groups, a total of 7 groups, including the following groups:

[0079] (1) Control group

[0080] Blank PBS group, carnosine 8000μg / ml group, palmitoyl tetrapeptide-7 0.7μg / ml group, nonapeptide-1 0.05μg / ml group

[0081] (2) Peptide composition experimental group

[0082] Carnosine 8000μg / ml+nonapeptide-1 0.05μg / ml group, palmitoyl tetrapeptide-7 0.7μg / ml+nonapeptide-1 0.05μg / ml group, carnosine 8000μg / ml+palmitoyl tetrapeptide-7 0.7μg / ml+nonapeptide -1 0.05 μg / ml group.

[0083] MTT detection of cell proliferation rate: the method is the same as in Example 1.

[0084] Determination of tyrosinase activity: the mouse melanoma cell B16 was mixed with 4×10 4 Each well was inoculated in a 96-well plate. After the cells adhered to the wall, the medium was changed once with fresh culture medium containing different polypeptides or polypeptide compositions, and three re...

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PUM

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Abstract

The invention discloses a polypeptide composition for inhibiting melanogenesis and an application thereof. The polypeptide composition is composed of one or two of an antioxidant polypeptide and a polypeptide inhibiting release of inflammatory factors and an MC1-R antagonist. The mutual synergistic effect of the MC1-R antagonist, the antioxidant polypeptide and the polypeptide inhibiting release of the inflammatory factors in inhibition of melanogenesis is fully played, inhibition of single pathway or single substance in the process of melanogenesis is avoided, and the polypeptide compositionhas better whitening effect and safety. Therefore, the polypeptide composition can be used for preparing cosmetics and dermatologic drugs.

Description

technical field [0001] The invention relates to a polypeptide composition, in particular to a polypeptide composition and application for inhibiting melanogenesis. Background technique [0002] Melanin is a high-molecular biological pigment synthesized by melanocytes distributed in the basal layer of the epidermis and is the main factor affecting skin color. Melanocytes have special organelles - melanosomes, which are produced by the evolution of the endoplasmic reticulum and contain tyrosinase. Under the action of this enzyme, tyrosine is oxidized to dopa, and dopa is further oxidized to melanin , gradually forming melanosomes, and gradually blackening. The melanized melanosomes will be transported outward along the dendrites of melanocytes, and the melanosomes will be distributed in them through the phagocytosis or pinocytosis of keratinocytes, resulting in darkening of the skin surface. Or spots appear locally. [0003] Regarding the molecular mechanism of regulating m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/64A61K45/06A61K38/05A61K38/06A61K38/07A61K38/08A61Q19/02A61P17/00
CPCA61K8/64A61K38/05A61K38/06A61K38/07A61K38/08A61K45/06A61K2800/5922A61K2800/782A61K2800/91A61K2800/92A61Q19/02A61K2300/00
Inventor 杨祺曹春来许文威黄有泉周翠王康钊
Owner ZHUHAI UNITED LAB
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