Method for Screening Positive Cloned Strains Applied to Anaerobic Bacteria and Facultative Anaerobic Bacteria
A technology of facultative anaerobic bacteria and anaerobic bacteria, applied in the field of genetic engineering, can solve the problems of inability to degrade hydrogen peroxide, inability to destroy superoxide groups, etc., and achieve remarkable effects
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Embodiment 1
[0017] Example 1: Screening Escherichia coli SOD superoxide dismutase target gene in DH5α Bifidobacterium adolescentis Positive clone of ATCC15703
[0018] 1) from Escherichia coli DH5α extracts catalase and SOD genes respectively: Escherichia coli DH5α strain culture, use LB medium, 37 ° C test tube shaking shaker culture to OD660 about 0.6, extract the whole gene of the bacteria and store it in a -20 ° C refrigerator for later use; according to Escherichia coli The heme catalase gene sequence of DH5α was designed with upstream and downstream primers to extract the heme catalase gene and stored in a -20°C refrigerator for later use; according to Escherichia coli The SOD superoxide dismutase gene sequence of DH5α was designed with upstream and downstream primers to extract the SOD catalase gene and stored in a -20°C refrigerator for later use;
[0019] 2) from Bifidobacterium longum ATCC 15707 extracts promoter and terminator genes: Bifidobacterium longum The ATCC...
Embodiment 2
[0023] Example 2: Screening Escherichia coli SOD target gene in DH5α Lacterbaillus acidophilus Positive clone of ATCC4356
[0024] 1) from Escherichia coli DH5α extracts catalase and SOD genes respectively: Escherichia coli DH5α strain culture, use LB medium, 37 ° C test tube shaking shaker culture to OD660 about 0.6, extract the whole gene of the bacteria and store it in a -20 ° C refrigerator for later use; according to Escherichia coli The heme catalase gene sequence of DH5α was designed with upstream and downstream primers to extract the heme catalase gene and stored in a -20°C refrigerator for later use; according to Escherichia coli The SOD superoxide dismutase gene sequence of DH5α was designed with upstream and downstream primers to extract the SOD catalase gene and stored in a -20°C refrigerator for later use;
[0025] 2) from Lacterbaillus acidophilus ATCC4356 extracts promoter and terminator genes: Lacterbaillus acidophilus The ATCC4356 strain was cu...
Embodiment 3
[0029] Example 3: Screening Escherichia coli SOD superoxide dismutase target gene in DH5α Bifidobacterium adolescentis Positive clone of ATCC15703
[0030] 1) from Escherichia coli DH5α extracts catalase and SOD genes respectively: Escherichia coli DH5α strain culture, use LB medium, 37 ° C test tube shaking shaker culture to OD660 about 0.6, extract the whole gene of the bacteria and store it in a -20 ° C refrigerator for later use; according to Escherichia coli The heme catalase gene sequence of DH5α was designed with upstream and downstream primers to extract the heme catalase gene and stored in a -20°C refrigerator for later use; according to Escherichia coli The SOD superoxide dismutase gene sequence of DH5α was designed with upstream and downstream primers to extract the SOD catalase gene and stored in a -20°C refrigerator for later use;
[0031] 2) from Bifidobacterium longum ATCC 15707 extracts promoter and terminator genes: Bifidobacterium longum The ATCC...
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