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Preparation method and application of livestock composite probiotic feed

A technology of compound probiotics and poultry, which is applied in the field of poultry breeding, can solve the problems of abuse, achieve the effect of reducing the use, increasing the acid production of fermented materials and probiotic live bacteria, and promoting the growth of poultry

Inactive Publication Date: 2018-06-01
广东科贸职业学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to overcome the problems of abuse of antibiotics in the existing poultry breeding, and provide a strain of Lactobacillus bulgaricus P-3, which can be added to the feed as a feed additive, and has the functions of improving the intestinal health of poultry and improving the health of poultry. Advantages of resistance and growth promotion of poultry

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Select 10 15-day-old healthy meat pigeons, first put the normal meat pigeons to death, use an inoculation loop to scrape the intestines and crop contents of the meat pigeons, and put them in sterile saline, and then use the inoculation loops to continuously mark them. Line inoculated into MRS medium, placed at 36°C for anaerobic culture for 48 hours, selected suspicious lactic acid bacteria on the plate, purified, and then streaked and inoculated in MRS and MC medium, MRS medium was anaerobically cultured at 36°C for 48 hours, MC medium Aerobic culture was carried out at 36°C for 48 hours, and the colony characteristics were observed after cultivation. According to "Lactic Acid Bacteria Classification and Identification and Experimental Methods" and "Common Bacteria System Identification Manual", observe the morphological characteristics and physiological and biochemical identification of the isolated strains.

[0025] After the isolated and purified lactic acid bacteri...

Embodiment 2

[0029] To prepare poultry feed:

[0030] (1) Preparation of fermentation broth: heat water to 40-50°C, add 2-8wt% food-grade glucose, add 0.1-0.5wt% lactic acid bacteria, 0.1-0.5wt% saccharomyces, 0.1-0.5wt% bacillus , stirred to dissolve, cultivated for 18-24 hours,

[0031] (2) Culture medium (material) preparation: soybean meal, corn, and broken rice are crushed and sieved, and set aside.

[0032] (3) Inoculation: After testing that the pH of the fermented liquid is less than or equal to 4.0, it can be inoculated. 10-25% of crushed and sieved soybean meal, 10-25% of corn, and 10-30% of broken rice are stirred in a mixer for 3-6 minutes. (1) 30-45% of the obtained fermented liquid, stirred for 1-5 minutes, mixed evenly and packaged.

[0033] (4) Fermentation: The sealed culture is sent to the fermentation workshop, and fermented at a constant temperature of 25-35° C. for 3 days.

[0034] (5) Acceptance of fermented material: After testing, PH ≤ 4.5, lactic acid bacteria ≥...

experiment example 1

[0038] Feeding test: select the 0-day-old healthy squabs produced by 4000 pairs of healthy breeding pigeons (500 pairs per warehouse) in the eight warehouses of the meat pigeon farm to test. Among them, seven warehouse pigeons were the test group (Group I), and the eighth warehouse pigeon was the control group (Group II). The feeding conditions are cages, and the feeding diet, medicine, drinking water, health care sand and other management conditions are the same. The eggs of the breeding pigeons were hatched in the hatching room, and the 0-day-old healthy pigeons were taken back from the hatching room and fed with the breeding pigeons, 4 in each nest. Group I was fed with conventional rations; Group Ⅱ was on the basis of conventional rations, adding the poultry feed made in 5% of the total weight in Example 2; during the test, it was fed twice a day with free Feed and drink, the light is sunlight, observe and record the mental state and food intake of the pigeons every day, ...

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PUM

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Abstract

The invention discloses a preparation method and application of livestock composite probiotic feed. The livestock composite probiotic feed comprises a lactobacillus bulgaricus P-3 strain, the taxonomyname of the strain is Lactobacillus bulgaricus which is preserved in the Guangdong Culture Collection center, the preservation number is GDMCC No: 60252, the preservation address is Floor 5, Building59, #100 Courtyard, Middle Xianlie Road, Guangzhou, and the preservation date is October 20, 2017. The lactobacillus bulgaricus P-3 strain can be used as a feed additive applied to livestock feed, iscapable of promoting growth of livestock, increasing nutrition levels of livestock, increasing feed benefits, reducing feed-to-meat ratio, increasing breeding benefits, reducing amounts of antibiotics of livestock breeding, improving immune functions and anti-stress properties of livestock, reducing death rates and achieving safe and effective green production of livestock products, has an inhibition effect on pathogenic bacteria, and has good application prospects and market values in maintaining and promoting healthy and stable development of the livestock breeding industry.

Description

technical field [0001] The invention belongs to the technical field of poultry breeding. More specifically, the invention relates to Lactobacillus bulgaricus P-3 strain and its application in preparing compound probiotic feed for poultry. Background technique [0002] With the increasing demand for poultry (pigeon) in domestic and foreign markets, my country's poultry industry is developing rapidly towards scale, product commercialization and management enterprise. At present, there are more than 30 million pairs of breeding pigeons in the country, with an annual output of about 620 million pigeons, growing at a rate of 10%-15% every year. At the same time, the occurrence and prevalence of poultry diseases are complicated, and food safety problems caused by irrational use of drugs in production have attracted people's attention. Contents of the invention [0003] The purpose of the present invention is to overcome the problems of abuse of antibiotics in the existing poult...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K50/75A23K50/70A23K10/18C12R1/225
CPCA23K10/18A23K50/70A23K50/75C12N1/205C12R2001/225
Inventor 邹永新李殿鑫黄笑筠肖智远冯开容郑秀容李剑杰姜文联张君李莉娜赖秋英康艳梅梁日勤
Owner 广东科贸职业学院
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