Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

BRCA1, JAML as well as application of regulatory gene thereof to variety breeding

A gene and pork quality technology, applied in the field of molecular biology, can solve problems such as no report prompting connection

Active Publication Date: 2018-06-01
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
View PDF2 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that JAML is expressed in various innate and adaptive immune cells, but there are no reports suggesting a link between it and muscle fat

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • BRCA1, JAML as well as application of regulatory gene thereof to variety breeding
  • BRCA1, JAML as well as application of regulatory gene thereof to variety breeding
  • BRCA1, JAML as well as application of regulatory gene thereof to variety breeding

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 Sample collection preparation and experimental design

[0041] After the experimental pigs were slaughtered, the fat tissue in the longissimus dorsi muscle was quickly collected, cut into small pieces, filled into 5mL cryopreservation tubes, frozen in liquid nitrogen, and then transferred to a -80°C refrigerator for long-term storage for total RNA The extraction, the experiment set up 3 groups, identified the lncRNA in the large white pig intramuscular adipose tissue (D_JN) and the Laiwu pig intramuscular adipose tissue (L_JN), and analyzed their intramuscular adipose tissue gene expression profiles, each sample set 3 repetitions.

Embodiment 2

[0042] Example 2 Extraction and quality control of sample total RNA

[0043] Take out the same amount of cryopreserved adipose tissue samples respectively, and use mirVana according to the instruction manual TM The RNA extraction kit was used to extract total RNA from each adipose tissue sample, and the isolated total RNA sample was stored in a -80°C refrigerator. Use the NanoDrop 2000 spectrophotometer to measure the concentration and OD260nm / OD280nm value of the RNA sample, and control it between 1.9 and 2.1, use the Bioanalyzer 2100 to evaluate the quality of the total RNA, and control RIN>=7 and 28S / 18S>=0.7, use RNase-free DNase I eliminates potential genomic DNA contamination.

Embodiment 3

[0044] Example 3 cDNA library construction and RNA sequencing

[0045] strand-specific cDNA library

[0046] (1) Ribo-zero kit removes rRNA

[0047] (2) RNA fragmentation

[0048] (3) Double-stranded cDNA synthesis and purification

[0049] (4) End repair, adding A base

[0050] (5) Sequencing adapter connection

[0051] (6) Enrichment and purification of DNA fragments

[0052] (7) Library Quality Inspection

[0053] (8) A total of 6 cDNA libraries were established in this study, namely D_JN_1, D_JN_2, D_JN_3 (Large White pig intramuscular adipose tissue cDNA library) and L_JN_1, L_JN_2, L_JN_3 (Laiwu pig intramuscular adipose tissue cDNA library).

[0054] RNA-Seq (Illumina Sequence)

[0055] After passing the quality inspection of the library, the Illumina HiSeqTM 2500 sequencing platform was used to perform sequencing analysis on the cDNA library using Paired-end Sequencing, and the off-machine data was the original sequencing data raw reads.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to BRCA1, JAML as well as a regulatory gene thereof to variety breeding, in particular to application of BRCA1, JAML and XLOC_011001 in breeding high-muscle-quality livestock andpoultry variety. The inventor discovers a BRCA1 gene and a JAML gene related to intramuscular fat of pork, and further analyzes and displays that XLOC_011001 is 1ncRNA regulating the expression of the two genes. The invention discloses molecular markers regulating the intramuscular fat deposition of the pork, and further studies the interaction relationship of the molecular markers, a result shows that the molecular markers can be used for breeding high-meat-quality livestock and poultry varieties, and have good application prospect in the breeding of animal husbandry.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and specifically relates to the application of BRCA1, JAML and regulatory genes thereof in breed selection, and more specifically relates to the application of BRCA1, JAML and XLOC_011001 in breeding high-muscle quality livestock and poultry breeds. Background technique [0002] The BRCA1 gene is an important tumor suppressor gene in the human body, and participates in key steps in life activities such as DNA repair, cell cycle regulation point control, mitosis and cell differentiation. BRCA1 gene mutation is closely related to the risk of breast cancer, and can also be used as a predictive gene for the efficacy of radiotherapy and chemotherapy for non-small cell lung cancer. However, there are few studies on this gene in animals, let alone its relationship with intramuscular fat. relation. [0003] JAML is a junctional adhesion molecule-like protein, a secreted type I transmembrane gl...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888G01N33/68C12N15/113C12N15/11
CPCC12N15/113C12Q1/6888G01N33/68C12Q2600/158C12Q2600/178C12Q2600/124A01K11/00C12Q1/6827C12Q1/6816A01K2227/108C12Q1/6851C12Q1/686
Inventor 苗向阳黄万龙李嫒解领丽张秀秀
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
Features
  • Generate Ideas
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com